MoFlo XDP High Speed Cell Sorter

MoFlo XDP 高速细胞分选仪

• 批准号: 7793781
• 负责人: MARKUS MOHRS
• 金额: $ 49.92万
• 依托单位: TRUDEAU INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-03 至 2011-07-02
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793781
• 关键词: Acute; Architecture; Basophils; Cell physiology; Cells; Commit; Core Facility; Dendritic Cells; Ensure; Flow Cytometry; Fluorescence; Fluorochrome; Funding; Future; Housing; Human Resources; Immune; Immune system; Immunity; Individual; Infection; Institutes; Invaded; Manufacturer Name; Memory; Methods; Modification; Phenotype; Population; Principal Investigator; Procedures; Property; Proteins; Research; Research Personnel; Services; Sorting - Cell Movement; Speed; T-Lymphocyte Subsets; Time; Transgenic Animals; base; combinatorial; design; fluorescence activated cell sorter device; instrument; magnetic beads; pathogen; population based; public health relevance; research study; response; vaccine development

DESCRIPTION (provided by applicant): The cells of the immune system are indispensable to protect us from invading pathogens and provide long-lasting immunity to re-infection. Essentially all of the 15 principal investigators at Trudeau Institute are funded and dedicated to study the fundamental mechanisms governing acute and long-lasting immune cell responses to infection and explore their potential for vaccine development. In order to study the properties and functions of these cells it is required to isolate distinct populations based on their unique phenotype. However, many of these immune cells, such as memory T cell subsets, dendritic cell subsets or basophils, are very rare and can only be identified by a combination of numerous phenotypic markers. Moreover, the increasing use of transgenic animals carrying fluorescent marker proteins excludes the use of non-fluorescent isolation methods such as magnetic beads. For these reasons a high speed multi-parameter fluorescence activated cell sorter is indispensable to effectively sort infrequent immune cells based on a combinatorial array of fluorescence parameters. While all investigators at Trudeau Institute have been using a cell sorter for many years the existing instrument is almost a decade old and poses critical limitations with respect to speed, the number of usable fluorochromes as well as the service and parts support by the manufacturer which will expire by the end of 2010. The requested MoFlo XDP High Speed Cell Sorter will overcome all of these limitations and will allow us a) to sort at significantly higher speed, which is critical to sort rare populations and generate more user time due to shortened sorts, b) to sort based on more independent fluorescence parameters, which is critical for the identification of particular immune cell subsets, c) to increase the effective sorting available to all investigators due to a significantly shortened setup period, d) ensure continued service and parts support by the manufacturer, Beckman Coulter. Although the individual experiments and procedures used by the investigators will likely change over time, the open architecture of the requested sorter was designed by the manufacturer to facilitate future modifications as needed. This instrument will be exclusively operated and maintained by the dedicated full-time personnel of the Flow Cytometry Core Facility. Because the research efforts of essentially all investigators are critically dependent on a high speed cell sorter Trudeau Institute is fully committed to provide all the necessary support to house and maintain this instrument in the future. PUBLIC HEALTH RELEVANCE: The cells of the immune system are indispensable for protecting us from invading pathogens and provide long-lasting immunity to re-infection. Many of these cells are rare and can only be identified by a combination of numerous parameters. In order to study the properties and functions of these cells we need to be able to isolate distinct populations based on their unique features.

描述（由申请人提供）：免疫系统的细胞是不可或缺的，以保护我们免受入侵的病原体，并提供持久的免疫力，以再次感染。特鲁多研究所的15名主要研究人员基本上都得到了资助，并致力于研究控制急性和持久免疫细胞对感染反应的基本机制，并探索它们在疫苗开发中的潜力。为了研究这些细胞的特性和功能，需要基于其独特的表型分离不同的群体。然而，许多这些免疫细胞，如记忆T细胞亚群，树突状细胞亚群或嗜碱性粒细胞，是非常罕见的，只能通过许多表型标志物的组合来识别。此外，越来越多地使用携带荧光标记蛋白的转基因动物排除了使用非荧光分离方法，如磁珠。由于这些原因，高速多参数荧光激活细胞分选仪对于基于荧光参数的组合阵列有效地分选罕见免疫细胞是必不可少的。虽然特鲁多研究所的所有研究人员多年来一直使用细胞分选仪，但现有仪器已使用近十年，在速度、可用荧光染料数量以及制造商提供的服务和零件支持方面存在严重限制，这些限制将于2010年底到期。所要求的MoFlo XDP高速细胞分选仪将克服所有这些限制，并将允许我们a）以显著更高的速度进行分选，这对于分选稀有群体至关重要，并由于缩短了分选而产生更多的用户时间，B）基于更独立的荧光参数进行分选，这对于鉴定特定的免疫细胞亚群至关重要，c）由于设置时间大大缩短，因此增加了所有研究者的有效分类，d）确保制造商Beckman Coulter的持续服务和部件支持。虽然研究人员使用的个别实验和程序可能会随着时间的推移而改变，但制造商设计了所需分拣机的开放式架构，以便于将来根据需要进行修改。该仪器将由流式细胞仪核心机构的专职专职人员专门操作和维护。由于基本上所有研究人员的研究工作都严重依赖于高速细胞分选机，特鲁多研究所完全致力于提供所有必要的支持，以容纳和维护这一仪器在未来。 公共卫生关系：免疫系统的细胞对于保护我们免受入侵病原体的侵害是不可或缺的，并为再次感染提供持久的免疫力。这些细胞中的许多是罕见的，只能通过许多参数的组合来识别。为了研究这些细胞的特性和功能，我们需要能够根据其独特的特征分离不同的群体。