Request for a Zeiss LSM 710 Confocal Microscope

描述（由申请人提供）：本申请申请资金用于购买新的蔡司LSM 710激光扫描共聚焦显微镜。该仪器将被安置在罗切斯特大学医学中心生物医学遗传学系指定的共聚焦室中。新的蔡司LSM 710将取代现有的徕卡TCS SP2共聚焦显微镜，该显微镜已经使用了10年，最近一直在维修。这种旧型号缺乏某些功能（如紫外线激光器和多通道探测器），而且升级成本太高。随着美国国立卫生研究院资助的用户数量在生物医学遗传学部门内不断增长，缺乏足够的共聚焦显微镜已成为许多美国国立卫生研究院资助项目进展中数据获取的主要限制因素。共聚焦显微镜目前存在于医疗中心的核心设施不能为医疗中心的所有用户提供及时的访问，医疗中心由40多个部门和研究中心组成。我们部门必须有自己的共聚焦显微镜来满足我们的成像需要。事实上，我们部门的共聚焦镜一直是使用最频繁的设备，在过去的几年里一直被全天候预订。该部门的研究人员认为共聚焦访问是他们实验中的一个限制步骤，也是造成相当大挫折的原因。目前的徕卡TCS SP2共焦在大量使用下老化，导致停机频率和时间增加，这让我们部门的研究人员更加沮丧。获得一种新的共聚焦显微镜将有助于缓解目前的压力，并加速多个美国国立卫生研究院资助的研究项目的数据采集。共聚焦显微镜已成为生物医学研究中成像研究的主要工具。该申请寻求资金以替换罗切斯特大学医学中心生物医学遗传学系的旧型号仪器。这种新仪器将是该部门唯一可用的共聚焦显微镜，并且将成为该部门许多美国国立卫生研究院资助的研究人员获取数据的必要工具。

DESCRIPTION (provided by applicant): This application requests funds for purchasing a new Zeiss LSM 710 laser scanning confocal microscope. This instrument will be housed in the designated confocal room in the Department of Biomedical Genetics at the University of Rochester Medical Center. The new Zeiss LSM 710 will replace an existing Leica TCS SP2 confocal microscope that has been in use for 10 years and has been constantly under repair recently. This older model lacks certain capabilities (such as a UV laser and a multi-channel detector) and is too costly to upgrade. As the number of NIH-funded users continues to grow within the Department of Biomedical Genetics, the lack of an adequate confocal microscope has become a major limiting factor in data acquisition for the progression of many NIH-funded projects. The confocal microscope currently exists in the Medical Center Core Facilities cannot provide timely access for all users in the Medical Center, which consists of over 40 departments and research centers. It is essential that our department have its own confocal microscope for our imaging needs. In fact, our departmental confocal has been the most heavily used piece of equipment and has been booked around the clock for the past years. Researchers in the department consider confocal access a limiting step in their experimentation and a cause of considerable frustration. The increased frequency and duration of downtime as the current Leica TCS SP2 confocal aged under heavy use have caused even more frustration among researchers in our department. The acquisition of a new confocal microscope will help alleviate the current strain and accelerate data acquisition in multiple NIH-funded research projects. Confocal microscopy has become a primary tool for imaging studies in biomedical research. This application seeks funds to replace an older model instrument within the Department of Biomedical Genetics at the University of Rochester Medical Center. This new instrument will be the only confocal microscope available in the department and will be essential for data acquisition for the many NIH-funded researchers of the department.