Acquisition of a modern continuous wave EPR spectrometer equipped with an ENDOR s

购买配备 ENDOR s 的现代连续波 EPR 光谱仪

• 批准号: 7791775
• 负责人: Andrei Astachkine
• 金额: $ 39.17万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-10 至 2011-12-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7791775
• 关键词: Affect; Aging; Antineoplastic Agents; Arizona; Binding; Biochemical; Biochemistry; Biological Process; Biomedical Research; Chemistry; Electron Nuclear Double Resonance; Electron Spin Resonance Spectroscopy; Ensure; Failure; Funding; Future; Health; Homeostasis; Human; Metals; Molybdenum; Nitric Oxide; Point Mutation; Probability; Proteins; Request for Proposals; Research; Research Personnel; Research Project Grants; Resistance; Ribonucleotide Reductase; Sulfites; System; Testing; Time; Universities; Walkers; enzyme model; instrument; oligomycin sensitivity-conferring protein; research study; sulfite oxidase

DESCRIPTION (provided by applicant): This proposal requests funding for acquisition of a new, modern continuous wave (CW) X-band (~9 GHz) electron paramagnetic resonance (EPR) spectrometer, Bruker Elexsys E500 equipped with E560D electron-nuclear double resonance (ENDOR) system, to support the NIH-funded biochemical/biomedical research at the University of Arizona. This spectrometer will be integrated into the existing EPR Facility at the Department of Chemistry and Biochemistry of the University of Arizona, where it will replace the existing aging (~20 years old) Bruker ESP300E CW EPR instrument. The old spectrometer has developed hardware problems, many of its components are completely outdated, and there is a high probability of a critical failure at any time. Such a failure will impede the progress of the projects of the NIH-funded users relying on EPR in their research. The replacement of the old spectrometer by a new, modern one will ensure an uninterrupted access of the users to EPR and will enrich their research capabilities through a significantly (up to an order of magnitude) higher sensitivity of the new instrument and through providing access to CW ENDOR experiments. In short term, this spectrometer will be used in the ongoing biochemical/biomedical research projects of the users who contributed to this proposal. In the long term, this spectrometer will answer the need in EPR experiments of the future NIH-funded researchers and other users of the EPR Facility at the University of Arizona. The specific affected biochemical/biomedical projects described in this proposal include: (1) Using EPR and CW ENDOR to study the molybdenum catalytic center of sulfite-oxidizing enzymes and model synthetic Mo(V) complexes by Dr. J.H. Enemark. This research is important because some point mutations of human sulfite oxidase has fatal consequences; (2) Using EPR for understanding structural and dynamic aspects of chemistry of nitric oxide, NO, which has numerous biological functions important for human health by Drs. F.A. Walker, K.M. Miranda and W.R. Montfort; (3) Using EPR to test the efficiency of newly developed potential cancer drugs suppressing the function of ribonucleotide reductase by Dr. R.T.Dorr; (4) Using EPR to study Cu binding to proteins in relation to metal homeostasis and metal resistance by Dr. M. McEvoy.

描述（由申请人提供）：该提案要求资助购买一种新型的现代连续波（CW）X波段（~9 GHz）电子顺磁共振（EPR）光谱仪，配备E560 D电子-核双共振（ENDOR）系统的Bruker Elexsys E500，以支持亚利桑那大学NIH资助的生物化学/生物医学研究。该光谱仪将被集成到亚利桑那大学化学和生物化学系现有的EPR设施中，取代现有老化（约20年）的Bruker ESP 300 E CW EPR仪器。旧的光谱仪已经出现了硬件问题，它的许多组件已经完全过时，并且随时都有很大的可能性发生临界故障。这样的失败将阻碍NIH资助的用户在其研究中依赖EPR的项目的进展。用新的现代化分光计取代旧分光计将确保用户不间断地获得EPR，并将通过新仪器的高得多的灵敏度（高达一个数量级）和通过提供CW ENDOR实验来丰富他们的研究能力。在短期内，该光谱仪将用于参与该提案的用户正在进行的生物化学/生物医学研究项目。从长远来看，该光谱仪将满足未来NIH资助的研究人员和亚利桑那大学EPR设备的其他用户在EPR实验中的需求。本提案中所述的具体受影响的生物化学/生物医学项目包括：（1）J.H.博士利用EPR和CW ENDOR研究亚硫酸盐氧化酶的钼催化中心和模拟合成Mo（V）络合物。敌人这项研究很重要，因为人类亚硫酸盐氧化酶的一些点突变会产生致命的后果;（2）使用EPR来了解一氧化氮（NO）化学的结构和动态方面，NO具有许多对人类健康重要的生物学功能，作者：FA博士步行者，K.M.米兰达和W.R.蒙特福特;（3）利用EPR技术研究新型抗癌药物对核糖核苷酸还原酶的抑制作用;（4）利用EPR技术研究铜与蛋白质的结合与金属稳态和金属抗性的关系。麦克阿瑟