JEOL JEM-1400 Transmission Electron Microscope

描述（由申请人提供）：Wadsworth中心的一组研究人员正在申请资金购买JEOL JEM-1400透射电子显微镜（TEM）。该仪器的主要用户包括五个美国国立卫生研究院资助的实验室，它们在这方面有着广泛的记录，它们的特定目标需要使用多种传统薄切片和冷冻方法进行电子显微镜分析。直到2008年底，沃兹沃斯的两台显微镜都可以用于这些项目。其中一架有24年历史的飞利浦EM420已经达到了它的使用寿命，并在2008年底退役。另一台TEM是一台已有14年历史的蔡司910，尽管有全面的服务计划，但它已经变得如此不可靠，以至于它的停机时间（2008年为66%）对研究进展构成了重大障碍。此外，蔡司910不能用于以前在EM420上执行的冷冻工作。JEOL JEM-1400是蔡司910的理想替代品，因为它可以很容易地适应传统和低温操作模式。这台仪器是至关重要的，因为它接受一个样品夹，可以一次加载多达五个网格。对于执行大量串行部分分析的三个主要用户来说，这个特性代表了显著的功能改进。还包括具有成本效益的2k x 2k数码相机，特别是用于层析成像和单颗粒低温电镜项目。该工具将被纳入一个长期存在的EM核心，并由地方行政和监督委员会确保适当的使用和维护。沃兹沃斯中心坚定地致力于增加JEM-1400。作为支持的证据，该中心将提供一名国家资助的、经验丰富的研究科学家来监督仪器的运行，承担与年度服务合同相关的费用，并提供额外的资金来购买五个主要用户中的三个所需的冷冻级、控制器和干泵站设备。此外，如果时间允许，该仪器将在专用EM核心的帮助下提供给其他资助的研究人员，从而增强沃兹沃思中心长期使用电子显微镜作为研究工具。

DESCRIPTION (provided by applicant): A group of investigators at the Wadsworth Center are requesting funds to purchase a JEOL JEM-1400 transmission electron microscope (TEM). The major users of this instrument include five NIH-funded laboratories with extensive track records in, and whose specific aims require analyses by electron microscopy using multiple conventional thin-section and cryo approaches. Until the end of 2008, two microscopes at Wadsworth were available for these projects. One of these, a 24-year-old Philips EM420, has reached the end of its service life, and was decommissioned at the end of 2008. The other TEM, a 14- year-old Zeiss 910 that, despite being covered by a comprehensive service plan, has become so unreliable that its downtime (66% in 2008) represents a significant barrier to research progress. Moreover, the Zeiss 910 cannot be used for the cryo work formerly performed on the EM420. The JEOL JEM-1400 is an ideal replacement for the Zeiss 910, because it will readily accommodate both conventional and cryo modes of operation. This instrument is vital as it accepts a specimen holder that can load up to five grids at one time. For the three major users that perform extensive serial-section analyses, this feature represents a significant functional improvement. A cost-effective 2k x 2k digital camera is also included, particularly for the tomographic and single-particle cryo-EM projects. This instrument will be integrated into a long- standing EM Core, with local administrative and oversight committees to ensure appropriate use and maintenance. The Wadsworth Center is strongly committed to the addition of the JEM-1400. As evidence of support, the Center will provide a State-funded, experienced, Research Scientist to oversee the instrument's operation, assume costs associated with the annual service contract, and provide additional funds to purchase the cryo stage, controller, and dry pumping station equipment that are required for three of the five major users. In addition, and as time permits, this instrument will be made available to other funded investigators with assistance from a dedicated EM Core, thus enhancing the Wadsworth Center's long-standing use of electron microscopy as a research tool.