JEOL JEM-1400 Transmission Electron Microscope

JEOL JEM-1400 透射电子显微镜

• 批准号: 7780652
• 负责人: MICHAEL P KOONCE
• 金额: $ 50万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-27 至 2011-05-26
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7780652
• 关键词: 14 year old; Commit; Contract Services; Electron Microscope; Electron Microscopy; Ensure; Equipment; Funding; Laboratories; Life; Maintenance; Microscope; Microtomy; Pump; Records; Research; Research Personnel; Scientist; Services; Specimen; Staging; Time; Work; cost; digital; experience; functional improvement; instrument; operation; particle; tool; transmission process

DESCRIPTION (provided by applicant): A group of investigators at the Wadsworth Center are requesting funds to purchase a JEOL JEM-1400 transmission electron microscope (TEM). The major users of this instrument include five NIH-funded laboratories with extensive track records in, and whose specific aims require analyses by electron microscopy using multiple conventional thin-section and cryo approaches. Until the end of 2008, two microscopes at Wadsworth were available for these projects. One of these, a 24-year-old Philips EM420, has reached the end of its service life, and was decommissioned at the end of 2008. The other TEM, a 14- year-old Zeiss 910 that, despite being covered by a comprehensive service plan, has become so unreliable that its downtime (66% in 2008) represents a significant barrier to research progress. Moreover, the Zeiss 910 cannot be used for the cryo work formerly performed on the EM420. The JEOL JEM-1400 is an ideal replacement for the Zeiss 910, because it will readily accommodate both conventional and cryo modes of operation. This instrument is vital as it accepts a specimen holder that can load up to five grids at one time. For the three major users that perform extensive serial-section analyses, this feature represents a significant functional improvement. A cost-effective 2k x 2k digital camera is also included, particularly for the tomographic and single-particle cryo-EM projects. This instrument will be integrated into a long- standing EM Core, with local administrative and oversight committees to ensure appropriate use and maintenance. The Wadsworth Center is strongly committed to the addition of the JEM-1400. As evidence of support, the Center will provide a State-funded, experienced, Research Scientist to oversee the instrument's operation, assume costs associated with the annual service contract, and provide additional funds to purchase the cryo stage, controller, and dry pumping station equipment that are required for three of the five major users. In addition, and as time permits, this instrument will be made available to other funded investigators with assistance from a dedicated EM Core, thus enhancing the Wadsworth Center's long-standing use of electron microscopy as a research tool.

描述（由申请人提供）：沃兹沃斯中心的一组研究人员正在申请资金购买JEOL JEM-1400透射电子显微镜（TEM）。该仪器的主要用户包括五个NIH资助的实验室，这些实验室在2005年有着广泛的跟踪记录，其具体目标需要使用多种常规薄片和冷冻方法进行电子显微镜分析。直到2008年底，沃兹沃斯的两台显微镜可用于这些项目。其中一台使用了24年的飞利浦EM 420已经达到使用寿命，并于2008年底退役。另一台TEM是一台14年前的蔡司910，尽管有一个全面的服务计划，但它已经变得非常不可靠，以至于它的停机时间（2008年为66%）是研究进展的一个重大障碍。此外，Zeiss 910不能用于以前在EM 420上执行的低温工作。JEOL JEM-1400是蔡司910的理想替代品，因为它可以很容易地适应常规和低温操作模式。该仪器非常重要，因为它可以接受一个样品保持器，一次最多可以加载五个网格。对于三个执行大量序列截面分析的主要用户来说，此功能代表了一个重要的功能改进。还包括一个具有成本效益的2k x 2k数码相机，特别是用于断层扫描和单粒子冷冻EM项目。这一工具将被纳入一个长期的环境管理核心，由地方行政和监督委员会确保适当使用和维护。沃兹沃斯中心坚决致力于增加JEM-1400。作为支持的证据，该中心将提供一名国家资助的、经验丰富的研究科学家来监督仪器的运行，承担与年度服务合同相关的费用，并提供额外资金来购买五个主要用户中的三个所需的低温阶段、控制器和干泵站设备。此外，在时间允许的情况下，该仪器将在专用EM核心的协助下提供给其他受资助的研究人员，从而增强沃兹沃斯中心长期使用电子显微镜作为研究工具的能力。