Shared Equipment Grant for Purchase of a Leica TCS SP5 Confocal Microscope

用于购买 Leica TCS SP5 共焦显微镜的共享设备补助金

• 批准号: 7793767
• 负责人: John B. Reinitz
• 金额: $ 46.41万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-20 至 2011-05-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793767
• 关键词: 8 year old; Address; Affect; Argon; Data; Equipment; Fluorescence Resonance Energy Transfer; Funding; Grant; Image; Lasers; Microscope; Productivity; Protocols documentation; Request for Proposals; Research; Research Personnel; Scanning; Scientist; Specialist; Staining method; Stains; System; Universities; developmental genetics; meetings; operation; research study

DESCRIPTION (provided by applicant): This proposal requests funds for the purchase of a Leica TCS SP5 confocal microscope. The Leica TCS SP5 is a single point broadband confocal modular platform equipped with an RGB (HeNe 633nm, HeNe 594nm, HeNe 543, Argon 458/476/488/496/514) and a UV-diode laser (405 nm). It is being requested for the purpose of upgrading the confocal facility at the SBU Center for Developmental Genetics (CDG). The current SP2 system available to the CDG researchers is over eight years old and no longer meets the technical requirements of the center. The SP5 will address several difficulties currently affecting our researchers, thereby promoting the research objectives and productivity of nine distinguished scientists from Stony Brook University (SBU). The system will allow the center to remain competitive by providing researchers with increased sensitivity, fast-scanning capabilities, and a UV laser line for imaging, photo-activation, and FRET experiments. Management and operation of the confocal system will be provided by a technical specialist, who will also assist users in developing staining and imaging protocols for special projects, as well helping investigators in the analysis of confocal data.

描述（由申请人提供）：本提案要求资金用于购买Leica TCS SP 5共聚焦显微镜。Leica TCS SP 5是一个单点宽带共焦模块化平台，配备RGB（HeNe 633 nm，HeNe 594 nm，HeNe 543，Argon 458/476/488/496/514）和UV二极管激光器（405 nm）。它是为了升级SBU发育遗传学中心（CDG）的共聚焦设施而申请的。CDG研究人员目前使用的SP2系统已经使用了八年多，不再满足中心的技术要求。SP 5将解决目前影响我们研究人员的几个困难，从而促进斯托尼布鲁克大学（SBU）九位杰出科学家的研究目标和生产力。该系统将使该中心保持竞争力，为研究人员提供更高的灵敏度，快速扫描能力，以及用于成像，光活化和FRET实验的紫外激光线。共聚焦系统的管理和操作将由一名技术专家提供，他还将协助用户为特殊项目制定染色和成像协议，并帮助研究人员分析共聚焦数据。