Request for a Spectral Confocal Microscope

索取光谱共焦显微镜

• 批准号: 7793953
• 负责人: THOMAS C RICH
• 金额: $ 39.74万
• 依托单位: UNIVERSITY OF SOUTH ALABAMA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-06 至 2011-05-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793953
• 关键词: 5 year old; 9 year old; Age; Alabama; Area; Biology; Cancer Biology; Cells; Chronic Obstructive Airway Disease; Commit; Contract Services; Core Facility; Doctor of Philosophy; Ensure; Experimental Designs; Failure; Financial Support; Funding; Generations; Grant; Housing; Hypertension; Image; Individual; Lasers; Life; Lung; Measurement; Medicine; Microscope; National Center for Research Resources; Optics; Preparation; Research; Research Personnel; Resolution; Running; Scanning; Services; Speed; System; Time; United States National Institutes of Health; Universities; Vascular Diseases; Vascular Smooth Muscle; Work; bioimaging; college; fluorophore; instrumentation; malignant breast neoplasm; programs; therapy development

DESCRIPTION (provided by applicant): Eleven NIH-funded investigators and one junior investigator with a pending NIH application are seeking support under the NCRR Shared Instrumentation Grant Program to obtain a Nikon A1 laser scanning confocal microscope. The Nikon microscope is justified because the two other confocal microscopes available at the University of South Alabama - a nine-year-old Leica TCS SP2 spectral confocal microscope and a five year old PerkinElmer Ultraview RS-3 spinning disk confocal microscope - are heavily used by thirty labs and more than fifty individuals. As such, access to these microscopes has become a rate-limiting step in conducting research. This limitation has worsened because increased usage and age have led to component failure and subsequent down time of the Leica system. In addition, neither microscope is capable of obtaining high resolution confocal images of preparations in environmental chambers or making live-cell, time lapse measurements of multiple fluorophores. The steering committee of the BioImaging Core Facility has identified the Nikon A1 laser scanning confocal microscope as the system best suited for our needs, because of its high acquisition speed, high-spatial resolution, incorporation of an acousto-optical tunable filter to control laser intensity, and ability to simultaneously acquire spectra divided into 32 separate wavelength bins of 2.5, 5, or 10 nm, and Nikon's excellent customer service at the University of South Alabama. The Nikon microscope will support a group of investigators conducting research in the areas of Lung Endothelial Biology, Vascular Smooth Muscle Biology, and Cancer Biology. As such, the work is highly relevant to understanding the underlying factors contributing to hypertension, vascular disease, COPD, and breast cancer, as well as the development of treatments for these conditions. The system will be housed in the BioImaging Core Facility with the two current confocal microscopes. The USA College of Medicine has committed to refurbish space, and to provide financial support for a three-year extended service contract with Nikon, and a Ph.D. level manager to run the BioImaging Core Facility. This individual will assist researchers with the use of the Nikon system, as well as other aspects of experimental design. The BioImaging Core Facility will ensure appropriate use and sharing of the system. The addition of the new generation confocal microscope will greatly expand the imaging capabilities available to NIH-funded researchers present on University of South Alabama campus.

描述(由申请人提供)：11名由NIH资助的研究人员和一名初级研究人员正在寻求NCRR共享仪器赠款计划的支持，以获得尼康A1激光扫描共聚焦显微镜。尼康显微镜是合理的，因为南阿拉巴马大学提供的另外两台共焦显微镜--已有9年历史的徕卡TCS SP2光谱共焦显微镜和已有5年历史的PerkinElmer Ultraview RS-3旋转盘共焦显微镜--被30个实验室和50多个人频繁使用。因此，接触这些显微镜已经成为进行研究的一个速度限制步骤。由于使用增加和使用年限增加，导致徕卡系统的部件故障和随后的停机时间，这一限制变得更加严重。此外，这两种显微镜都不能获得环境室内制剂的高分辨率共聚焦图像，也不能对多个荧光团进行活细胞、时间推移测量。生物成像核心设施指导委员会已确定尼康A1激光扫描共聚焦显微镜系统最适合我们的需求，因为它具有高采集速度、高空间分辨率、采用声光可调滤光器来控制激光强度、能够同时采集分为32个单独的2.5、5或10 nm波长箱的光谱，以及尼康在南阿拉巴马州大学的出色客户服务。尼康显微镜将支持一组研究人员在肺内皮生物学、血管平滑肌生物学和癌症生物学领域进行研究。因此，这项工作与了解导致高血压、血管疾病、慢性阻塞性肺病和乳腺癌的潜在因素以及开发针对这些疾病的治疗方法高度相关。该系统将与目前的两台共焦显微镜一起安置在生物成像核心设施中。美国医学院已经承诺整修空间，并为与尼康签订的为期三年的延长服务合同提供财政支持，以及一名博士级别的经理来运营生物成像核心设施。该人员将协助研究人员使用尼康系统，以及实验设计的其他方面。生物成像核心设施将确保适当使用和共享该系统。新一代共聚焦显微镜的加入将极大地扩展NIH资助的研究人员在南阿拉巴马大学校园内的成像能力。

项目成果

Dynamic Ca(2+) signal modalities in the vascular endothelium.

• DOI: 10.1111/j.1549-8719.2012.00180.x
• 发表时间: 2012-07
• 期刊: Microcirculation (New York, N.Y. : 1994)
• 作者: Taylor MS;Francis M;Qian X;Solodushko V
• 通讯作者: Solodushko V

ASIC subunit ratio and differential surface trafficking in the brain.

ASIC亚基比和大脑中的差异表面运输。

• DOI: 10.1186/s13041-016-0185-7
• 发表时间: 2016-01-08
• 期刊: Molecular brain
• 影响因子: 3.6
• 作者: Wu J;Xu Y;Jiang YQ;Xu J;Hu Y;Zha XM
• 通讯作者: Zha XM

Hyperspectral imaging microscopy for identification and quantitative analysis of fluorescently-labeled cells in highly autofluorescent tissue.

• DOI: 10.1002/jbio.201100066
• 发表时间: 2012-01
• 期刊: JOURNAL OF BIOPHOTONICS
• 影响因子: 2.8
• 作者: Leavesley, Silas J.;Annamdevula, Naga;Boni, John;Stocker, Samantha;Grant, Kristin;Troyanovsky, Boris;Rich, Thomas C.;Alvarez, Diego F.
• 通讯作者: Alvarez, Diego F.

The interaction between the first transmembrane domain and the thumb of ASIC1a is critical for its N-glycosylation and trafficking.

• DOI: 10.1371/journal.pone.0026909
• 发表时间: 2011
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Jing L;Jiang YQ;Jiang Q;Wang B;Chu XP;Zha XM
• 通讯作者: Zha XM

Decoding dynamic Ca(2+) signaling in the vascular endothelium.

解码血管内皮细胞中的动态 Ca(2+) 信号传导。

• DOI: 10.3389/fphys.2014.00447
• 发表时间: 2014
• 期刊: Frontiers in physiology
• 影响因子: 4
• 作者: Taylor MS;Francis M
• 通讯作者: Francis M