A Shared MicroCT Instrument for Virtual Histology Imaging of Bone and Soft Tissue

用于骨和软组织虚拟组织学成像的共享 MicroCT 仪器

• 批准号: 7793771
• 负责人: ROBERTO J FAJARDO
• 金额: $ 35.91万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-06 至 2012-05-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793771
• 关键词: 3-Dimensional; Architecture; Bone Density; Bone Mineral Contents; Cancer Model; Communities; Data; Detection; Development; Embryology; Equipment; Evaluation; Funding; Grant; Histology; Image; Institutes; Institution; Medicine; Methods; Microscopic; Morphologic artifacts; National Center for Research Resources; Neoplasm Metastasis; Noise; Provider; Research; Research Personnel; Resolution; Resort; Resource Sharing; Sampling; Scanning; Science; Services; South Texas; Specimen; Speed; System; Testing; United States National Institutes of Health; X-Ray Computed Tomography; angiogenesis; base; bone; bone imaging; comparative; design; innovation; instrument; instrumentation; pre-clinical; programs; soft tissue; three dimensional structure; ultra high resolution; virtual

DESCRIPTION (provided by applicant): The instrument requested in this application is an ultra-high resolution microscopic computed tomography (microCT) scanner to evaluate 3-dimensional bone and soft tissue architecture from ex vivo specimens. This instrument, a Skyscan 1172 microCT scanner, is designed for en bloc imaging of bone for histomorphometry and determination of bone mineral density and bone mineral content. In addition, taking advantage of a unique method of microCT-based soft tissue Virtual Histology developed at our institution, one can image 3-dimensional structures of soft tissue for a wide variety of biomedical applications that include embryology, detection of micro-metastases in preclinical cancer models, and evaluation of neocapillaries during angiogenesis. None of the microCT equipment available in the South Texas region is capable of achieving the same resolution as the SkyScan 1172. Users who have attempted to collect data on the other instruments have been frustrated by the inability to capture data with sufficient detail and have therefore resorted to incurring the inconvenience and expense to send samples to commercial service providers. This situation is suboptimal due to that lack of biomedical expertise at the companies and the inability of the users to iteratively optimize scan parameters. Comparative tests of comparable systems for best spatial resolution, least noise, least artifacts, and best speed proved that the SkyScan 1172 was the most appropriate instrument to satisfy the needs of the UTHSCSA user community. As a shared resource for the UTHCSA campus, the SkyScan 1172 will be available to UTHSCSA investigators and partnering institutions throughout South Texas as a shared resource for the Institute for the Integration of Medicine and Science (NCRR CTSI, UL1 RR025767) as well as to academic investigators in other states. Immediately, the shared instrument would support 12 NIH grants as well as 10 other federally-funded regional and national grants however it is expected that a number of other research programs will take advantage of this system once it becomes available regionally. The development of microCT-based soft tissue Virtual Histology as a widely available application is considered a key innovation of this shared instrumentation grant.

描述（由申请人提供）：本申请中要求的仪器是一种超高分辨率显微计算机断层扫描（microCT）扫描仪，用于评价离体标本的三维骨和软组织结构。该仪器是一台Skyscan 1172 microCT扫描仪，设计用于骨的整体成像，以进行组织形态测量和骨矿物质密度和骨矿物质含量的测定。此外，利用我们机构开发的基于microCT的软组织虚拟组织学的独特方法，可以对软组织的三维结构进行成像，用于各种生物医学应用，包括胚胎学，临床前癌症模型中微转移的检测，以及血管生成过程中新冠的评估。南德克萨斯州地区现有的microCT设备都无法达到SkyScan 1172的分辨率。试图收集其他仪器数据的用户因无法获取足够详细的数据而感到沮丧，因此不得不承担向商业服务提供商发送样本的不便和费用。由于公司缺乏生物医学专业知识以及用户无法迭代地优化扫描参数，这种情况是次优的。对可比较系统进行的最佳空间分辨率、最小噪声、最小伪影和最佳速度的比较测试证明，SkyScan 1172是满足UTHSCSA用户群体需求的最合适的仪器。作为UTHCSA校园的共享资源，SkyScan 1172将提供给UTHCSA调查人员和整个南德克萨斯州的合作机构，作为医学与科学整合研究所（NCRR CTSI，UL1 RR025767）以及其他州的学术调查人员的共享资源。立即，共享工具将支持12个NIH赠款以及其他10个联邦资助的区域和国家赠款，但预计一旦该系统在区域范围内可用，其他一些研究项目将利用该系统。基于microCT的软组织虚拟组织学的开发作为一个广泛可用的应用程序被认为是这个共享仪器赠款的关键创新。