Roche 454 GL FLX DNA Sequencer

罗氏 454 GL FLX DNA 测序仪

基本信息

批准号：
7793003
负责人：
WILLIAM George FARMERIE
金额：
$ 50万
依托单位：
UNIVERSITY OF FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-03-18 至 2012-03-17
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7793003
关键词：
Agriculture Antihypertensive Agents Bioinformatics Biological Markers Biomedical Research Biotechnology Chromatin Chronic Core Facility DNA DNA Sequence Discipline Disease Progression Ensure Environmental Pollution Exhibits Florida Food Funding Gene Expression Profiling Genome Growth Human Human Resources Immunologic Deficiency Syndromes Infant Institutes Malignant Neoplasms Malignant neoplasm of prostate Metagenomics MicroRNAs Mycoplasma Infections Necrotizing Enterocolitis Neurons Occupations Pathogenesis Pharmacogenomics Play Prevention Research Research Infrastructure Research Personnel Research Project Grants Risk Role Science Services Stream Technology Transcriptional Activation United States National Institutes of Health Universities Variant Viral Virus Virus Diseases Wages Water base comparative disease diagnosis disease transmission instrument microbiome operation success

项目摘要

DESCRIPTION (provided by applicant): The University of Florida contributes substantially to the local and regional economy. In 2008, UF created 2,525 jobs and recent studies have shown that UF contributes nearly $6 billion annually to Florida's economy. The university employs about 34,000 people directly on its main campus and via UF organizations, such as the Institute of Food and Agricultural Sciences, is responsible for the creation of 74,894 jobs statewide. The current proposal will create or retain 3 jobs. We request $500,000 to purchase a pyrosequencing-based DNA sequencing instrument that will enable the UF Interdisciplinary Center for Biotechnology Research (ICBR) core facilities to support increasing demand by NIH-funded investigators. Currently, UF owns and operates one pyrosequencing instrument; purchased in 2005 that provides massively parallel DNA sequencing services to UF as well as other state and non-profit entities. The ICBR pyrosequencer is currently the only such instrument available in Florida. Over a period of nearly four years, we demonstrate dramatic growth in demand for pyrosequencing services. Now, our very success is in danger of saturating our pyrosequencing capacity. Acquisition of a second pyrosequencer aims to ensure NIH-funded investigators have ready access to this crucial technology. The potential health-related impact of their NIH-funded research projects is a reflection of the breadth of biomedical research disciplines represented by our major users. Their projects range from discovering biomarkers for environmental contaminants in our waters and streams, understanding immunodeficiency virus variation during disease progression, and the role viral and host genome variation plays in disease transmission, and the comparative metagenomics of the microbiome in human infants at risk for necrotizing enterocolitis. In between are projects for understanding the role of chronic mycoplasma infection in prostate cancer, comparative pharmacogenomics of antihypertensive therapy, discovery of factors promoting transcriptional activation in chromatin, gene expression profiling in single neurons, and the role of miRNA in latent viral infections and how this contributes to viral persistence and pathogenesis in cancers. The impact of supported NIH research projects will be felt in disease diagnosis, treatment, and prevention. The State of Florida and the University of Florida have demonstrated a commitment to operation of this instrument through continuous salary support for technical staff, acquisition of all ancillary instruments, as well as exhibiting significant commitments to computational hardware infrastructure and bioinformatics personnel.

描述（由申请人提供）：佛罗里达大学为地方和地区经济做出了重大贡献。 2008年，UF创造了2,525个就业机会，最近的研究表明，UF每年为佛罗里达州的经济贡献近60亿美元。该大学直接在其主要校园内和通过UF组织（例如食品和农业科学研究所）雇用约34,000名员工，负责全州创建74,894个职位。当前的建议将创建或保留3个工作。我们要求$ 500,000购买基于焦磷酸测序的DNA测序工具，该工具将使UF生物技术研究中心（ICBR）核心设施能够支持NIH资助的研究人员的需求不断提高。目前，UF拥有并运营着一种旋转式测序工具；于2005年购买，可为UF以及其他州和非营利实体提供大规模并行的DNA测序服务。 ICBR Pyrosequencer目前是佛罗里达州唯一可用的仪器。在近四年的时间里，我们证明了对焦磷酸素素测量服务的需求急剧增长。现在，我们的成功是使我们的焦磷酸测序能力饱和的危险。第二次旋齿杆菌的收购旨在确保NIH资助的调查人员可以准备好使用这项关键技术。其NIH资助的研究项目的潜在与健康相关的影响反映了我们主要用户代表的生物医学研究学科的广度。他们的项目范围从发现我们水域和溪流中环境污染物的生物标志物，了解疾病进展过程中的免疫缺陷病毒变化，以及疾病传播中的病毒和宿主基因组变异作用以及人类婴儿中微生物组的比较宏基因组学在人类婴儿中的比较宏基因组学风险。在两者之间是为了了解慢性支原体感染在前列腺癌中的作用，抗高血压疗法的比较药物基因组学，发现促进染色体上转录激活的因素，单个神经元中的基因表达分析以及miRNA在潜在病毒感染中的作用以及对病毒持久性持久性持久性和patationseasencence和pancerseasension和cancers的作用。在疾病诊断，治疗和预防方面，将感受到支持的NIH研究项目的影响。佛罗里达州和佛罗里达大学通过对技术人员的持续薪水支持，收购所有辅助工具以及对计算硬件基础设施和生物信息学人员的重大承诺，表现出对该工具运营的承诺。