BD LSR II Special Order System

BD LSR II 特别订购系统

• 批准号: 7794795
• 负责人: Kenneth Michael Pollard
• 金额: $ 50万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-01 至 2011-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794795
• 关键词: 8 year old; AIDS/HIV problem; Aging; Area; Asthma; Autoimmunity; Cell Shape; Cells; Color; Cytoskeleton; Disease; Flow Cytometry; Funding; Hour; Housing; Image; Influenza; Lasers; Malignant Neoplasms; Medicine; Molecular; Population; Protein-Carbohydrate Interaction; Qualifying; Research; Research Institute; Research Personnel; Sepsis; Services; Site; Stem cells; System; Technology; United States National Institutes of Health; Viola; Work; instrument; instrumentation; member; public health relevance; research study; tool

DESCRIPTION (provided by applicant): The instrument requested in this application is a configuration of the Becton Dickinson (BD) LSR II Flow Cytometer. This configuration of the BD LSR II has five lasers; (Blue laser/Red laser/Yellow-Green laser/Violet laser/UV laser) and the BD High Throughput Sampler (HTS). Nine qualified users have been identified with specific needs for advanced flow cytometry. All nine users are situated in two off-campus buildings which while close to each other are a significant distance from the on-campus Flow Cytometry Core which houses flow cytometers that have some of the required technology but are oversubscribed and often have to be accessed after normal working hours when technical support is not available. To service the growing need for analytical flow cytometry by investigators housed outside the main campus the on-campus Flow Cytometry Core has established two satellite facilities. The satellite flow facility that is part of the Department of Molecular and Experimental Medicine (MEM) is ideally situated as the site for the only off-campus LSR II cytometer due to the presence of designated space, technical support and a significant population of investigators who use flow cytometry in their research. This satellite facility houses two FACSCaliburs that are eight years old and use technology that is unable to support the growing need for experiments requiring greater than 4 colors. In addition they are incapable of performing the specialized tasks required by multiple members of the User Group. Access to a BD LSR II by off-campus investigators would provide much needed instrumentation and greatly facilitate NIH funded studies in the areas of influenza, asthma, sepsis, autoimmunity, HIV/AIDS, cancer, aging, stem cells, macromolecular imaging, cytoskeleton and cell shape, and protein-carbohydrate interactions. PUBLIC HEALTH RELEVANCE: The Becton Dickinson LSR II is a versatile and powerful flow cytometer capable of multi-color, multiparameter analysis. Such an instrument is an invaluable tool in identifying the numerous cell populations and their subsets that contribute to many diseases. The availability of such an instrument to investigators in the Department of Molecular and Experimental Medicine of the Scripps Research Institute will greatly facilitate cutting-edge research in the areas of influenza, asthma, sepsis, autoimmunity, HIV/AIDS,cancer, aging, stem cells, macromolecular imaging, cytoskeleton and cell shape, and proteincarbohydrate interactions.

描述(由申请人提供)：本申请中要求的仪器是Becton Dickinson(BD)LSR II流式细胞仪的配置。此配置的BD LSR II有五个激光器：(蓝光/红光/黄绿光/紫光/紫外光)和BD高通量采样器(HTS)。已确定9名符合条件的用户对先进的流式细胞术有特殊需求。所有9个用户都位于两个校外大楼，虽然彼此靠近，但与校园内流式细胞仪核心有很大距离，后者存放的流式细胞仪具有一些所需的技术，但订阅量过大，而且往往需要在正常工作时间之后才能访问，因为没有技术支持。为了满足主校区外的调查人员对流式细胞仪日益增长的需求，校内的流式细胞仪核心建立了两个卫星设施。作为分子与实验医学系(MEM)的一部分，卫星Flow设施是唯一校外LSR II细胞仪的理想选址，因为它有指定的空间、技术支持和大量使用流式细胞术进行研究的研究人员。这个卫星设施容纳了两个FACSCaliburs，它们已有8年的历史，所使用的技术无法支持日益增长的需要超过4种颜色的实验。此外，他们不能执行用户组多个成员所要求的专门任务。校外研究人员使用BD LSR II将提供急需的仪器，并极大地促进NIH资助的流感、哮喘、败血症、自身免疫、艾滋病毒/艾滋病、癌症、衰老、干细胞、大分子成像、细胞骨架和细胞形状以及蛋白质-碳水化合物相互作用等领域的研究。 公共卫生相关性：Becton Dickinson LSR II是一种多功能、功能强大的流式细胞仪，能够进行多颜色、多参数分析。这种仪器是确定导致许多疾病的众多细胞群及其亚群的无价工具。斯克里普斯研究所分子和实验医学部的研究人员可以使用这种仪器，这将极大地促进流感、哮喘、败血症、自身免疫、艾滋病毒/艾滋病、癌症、衰老、干细胞、大分子成像、细胞骨架和细胞形状以及蛋白质和碳水化合物相互作用等领域的尖端研究。