Request for electron microscope

索取电子显微镜

• 批准号: 7792771
• 负责人: Marian DiFiglia
• 金额: $ 19.42万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-01 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792771
• 关键词: Alzheimer' s Disease; Animal Disease Models; Area; Autophagocytosis; Cell Membrane Permeability; Communities; Demyelinations; Disease; Electron Microscope; Electron Microscopy; General Hospitals; Huntington Disease; Institution; Massachusetts; Nervous system structure; Neurodegenerative Disorders; Neurology; Neurons; Neurotransmitter Receptor; Parkinson Disease; Proteins; Records; Recycling; Research; Research Personnel; Role; Training; Traumatic Brain Injury; Work; digital imaging; improved; instrument; transmission process

DESCRIPTION (provided by applicant): This is a re-submission application for a request for a new transmission electron microscope (TEM) to replace an existing one that was purchased in 1979. Our current TEM has been operating for 28 years. It is failing and is wholly inadequate to serve the research needs of our large active and expanding research community in the Department of Neurology at Massachusetts General Hospital. Many of our researchers work in neurodegenerative diseases including Alzheimer disease, Huntington's disease, and Parkinson's disease. Some of the current exciting topics we work on include the role of autophagy in clearance of disease proteins from neurons, the effects of disease proteins on organization and recycling of neurotransmitter receptors in animal models of disease, membrane permeability changes in traumatic brain injury, and the mechanisms underlying axonal demyelination. These and other research areas will be greatly enhanced by having a new TEM in our department. Some of us have long track records in using electron microscopy to study the nervous system. The lack of a reliably functioning TEM hinders our ability to plan new directions involving EM analysis and in training our young investigators in EM applications. Advances in TEMs have greatly improved especially in capability for self-alignment and digital imaging, which we lack in our TEM. We do not have access to other modern instruments at our institution. Since the first submission, I have received an institutional commitment of $141,000 to be used toward the purchase of a new TEM. We have also improved our application by making the recommended revisions suggested by the reviewers.

描述(由申请人提供)：这是一份重新提交的申请，要求购买新的透射电子显微镜(TEM)以取代1979年购买的现有电子显微镜。我们目前的瞬变显微镜已经运行了28年。它是失败的，完全不足以满足我们在马萨诸塞州综合医院神经内科的庞大、活跃和不断扩大的研究社区的研究需求。我们的许多研究人员从事神经退行性疾病的研究，包括阿尔茨海默病、亨廷顿病和帕金森氏病。目前我们致力于的一些激动人心的主题包括自噬在清除神经元中的疾病蛋白中的作用，疾病蛋白对疾病动物模型中神经递质受体的组织和循环的影响，创伤性脑损伤中的膜通透性变化，以及轴突脱髓鞘的机制。这些和其他研究领域将大大加强，因为我们系有一个新的瞬变电磁波。我们中的一些人在使用电子显微镜研究神经系统方面有着长期的记录。缺乏可靠运行的瞬变电磁法阻碍了我们规划涉及EM分析的新方向的能力，以及在EM应用方面培训我们的年轻研究人员的能力。电子显微镜的进步已经大大提高，特别是在自对准和数字成像能力方面，这是我们的电子显微镜所缺乏的。在我们的机构中，我们无法获得其他现代工具。自从第一次提交以来，我已经收到了一笔141,000美元的机构承诺，将用于购买新的TEM机。我们还改进了我们的应用程序，根据评审员的建议进行了修改。

项目成果

Early whole-body mutant huntingtin lowering averts changes in proteins and lipids important for synapse function and white matter maintenance in the LacQ140 mouse model.

早期全身突变亨廷顿蛋白降低可避免蛋白质和脂质的变化，这对 LacQ140 小鼠模型中的突触功能和白质维持很重要。

• DOI: 10.1101/2023.01.26.525697
• 发表时间: 2023
• 期刊: bioRxiv : the preprint server for biology
• 作者: Shing,Kai;Sapp,Ellen;Boudi,Adel;Liu,Sophia;Seeley,Connor;Marchionini,Deanna;DiFiglia,Marian;Kegel-Gleason,KimberlyB
• 通讯作者: Kegel-Gleason,KimberlyB

Disposition of Proteins and Lipids in Synaptic Membrane Compartments Is Altered in Q175/Q7 Huntington's Disease Mouse Striatum.

• DOI: 10.3389/fnsyn.2021.618391
• 发表时间: 2021
• 期刊: Frontiers in synaptic neuroscience
• 影响因子: 3.7
• 作者: Iuliano M;Seeley C;Sapp E;Jones EL;Martin C;Li X;DiFiglia M;Kegel-Gleason KB
• 通讯作者: Kegel-Gleason KB