UAB Shared Biacore T100 Biosensor

UAB 共享 Biacore T100 生物传感器

• 批准号: 7793271
• 负责人: RANDALL S DAVIS
• 金额: $ 36.47万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-07 至 2011-01-06
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793271
• 关键词: Advisory Committees; Alabama; Area; Arts; Binding; Biosensor; Commit; Communities; Complement; Conflict (Psychology); Discipline; Ensure; Fees; Funding; Grant; Growth; Immunology; Kinetics; Laboratories; Leadership; Microbiology; Minor; Research; Research Institute; Research Personnel; Resources; Running; Schools; Scientist; Surface Plasmon Resonance; System; Technical Expertise; Training; Translational Research; Universities; Wages; base; cost; drug discovery; experience; innovation; instrument; instrumentation; interest; multidisciplinary; novel; operation; success

DESCRIPTION (provided by applicant): Defining the kinetics of molecular interactions between biomolecules has become an essential application for fundamental microbiology and immunology research and translational drug discovery. This shared instrumentation grant requests funding to purchase a state-of-the-art Biacore T100 surface plasmon resonance (SPR) system to establish a shared instrumentation facility at the University of Alabama at Birmingham (UAB). The instrument would serve an unmet need on campus for a base of eight major users (70%) and would be centrally located within the Shelby Interdisciplinary Biomedical Building in close proximity to the primary investigators' laboratories. This unit would replace a recently relocated Biacore 3000 instrument and complement an existing Biacore 2000 system that is restricted to a dedicated group of users in a different area of the UAB campus. The remaining availability of the instrument would be reserved for a significant pool of minor and outside users that includes the closely situated Southern Research Institute. The requested T100 unit and facility would thus provide some accessibility to the greater scientific community. We plan to enhance existing technical expertise by sending designates from all major users laboratories to Biacore for specific training. A dedicated facility manager with SPR experience will be hired to maintain, run, supervise, and further educate users. A broadly-based internal advisory committee will resolve any conflicts and identify strategic long-term support for the unit. Strong institutional support will assure the smooth start up and long-term success of the instrument and facility. Significant funds have been committed by the University and school leadership that will provide facility salary support and a long term five year extended warranty to ensure its optimal operation. User fees generated from use of the instrument will be applied towards salary support of the manager and cost of the facility. This new core would be an excellent multidisciplinary resource for established investigators in the immediate microbiology and immunology community as well as scientists in other disciplines with an interest in exploring innovative approaches for detailed characterization of molecular interactions. Acquisition of this instrument and establishment of this new core would provide a readily accessible state-of-the-art facility that would launch an outstanding platform for stimulating novel hypotheses and enhancing biomedical growth and advancement of NIH-funded research at UAB.

描述（由申请人提供）：定义生物分子之间分子相互作用的动力学已成为基础微生物学和免疫学研究以及转化药物发现的重要应用。这项共享仪器赠款要求提供资金，以购买最先进的Biacore T100表面等离子体共振（SPR）系统，在伯明翰的亚拉巴马大学（UAB）建立一个共享仪器设施。该仪器将满足校园内8个主要用户（70%）的未满足需求，并将位于谢尔比跨学科生物医学大楼的中心，靠近主要研究人员的实验室。该设备将取代最近搬迁的Biacore 3000仪器，并补充现有的Biacore 2000系统，该系统仅限于UAB校园不同区域的专用用户群。该仪器的其余可用性将保留给大量的小用户和外部用户，包括位置很近的南方研究所。因此，所要求的T100装置和设施将为广大科学界提供某种程度的无障碍环境。我们计划通过派遣所有主要用户实验室的指定人员到比亚科雷进行具体培训，加强现有的技术专长。将聘请一名具有SPR经验的专用设施经理来维护、运行、监督和进一步教育用户。一个基础广泛的内部咨询委员会将解决任何冲突，并确定对该股的战略性长期支助。强有力的机构支持将确保该文书和设施的顺利启动和长期成功。大学和学校领导层承诺提供大量资金，以提供设施工资支持和长期的五年延长保修，以确保其最佳运营。使用仪器产生的用户费将用于管理人员的工资支助和设施费用。这个新的核心将是一个很好的多学科资源，为建立在直接的微生物学和免疫学社区的研究人员，以及在其他学科的科学家有兴趣探索创新的方法，详细表征分子相互作用。收购这一工具和建立这个新的核心将提供一个易于访问的最先进的设施，将推出一个优秀的平台，刺激新的假设，并提高生物医学的增长和促进NIH资助的研究在UAB。

项目成果

Novel factor VIII variants with a modified furin cleavage site improve the efficacy of gene therapy for hemophilia A.

带有修饰的脂蛋白裂解位点的新因子VIII变体提高了基因疗法对血友病的疗效。

• DOI: 10.1111/jth.13543
• 发表时间: 2017-01
• 期刊: Journal of thrombosis and haemostasis : JTH
• 作者: Nguyen GN;George LA;Siner JI;Davidson RJ;Zander CB;Zheng XL;Arruda VR;Camire RM;Sabatino DE
• 通讯作者: Sabatino DE