Noninvasive Visualization and Analysis of Organ Systems using OCT and Digital Fun

使用 OCT 和 Digital Fun 进行器官系统的无创可视化和分析

• 批准号: 7793095
• 负责人: STEVEN J PITTLER
• 金额: $ 27.49万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-11-20 至 2010-11-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793095
• 关键词: Age; Animals; Brain; Cancer Center Support Grant; Cardiology; Cataract; Cations; Center Core Grants; Clinic; Complement; Crystallins; Cyclic GMP; Diagnostic; Diagnostic Imaging; Dimensions; Disease; Equipment; Eye; Eye Development; Funding; Fundus; Gastroenterology; Heart; Histologic; Housing; Human; Image; Imagery; Laboratory Research; Lead; Life; Medical; Microscope; Minor; Molecular and Cellular Biology; Ophthalmologist; Ophthalmology; Optic Nerve; Optical Coherence Tomography; Optometry; Protease Inhibitor; Research; Research Personnel; Retina; Retinal; Retinal Degeneration; Rhodopsin; Structure; Technology; Time; Tissues; biological systems; body system; cost; digital; digital imaging; imaging modality; in vivo; instrument; instrumentation; new technology; public health relevance; ranpirnase; research study; retinal rods; tomography; tool; trafficking; ultra high resolution; vision science

DESCRIPTION (provided by applicant): We propose the acquisition of Bioptigen, Inc. 840 nm SDOCT Optical Coherence Tomography (OCT) bundled with Phoenix Research Laboratories, Inc. Micron II Retinal Imaging Microscope package (total cost $274,940) for use with both humans and other animals. These instruments, which are currently not available at UAB will make an essential addition to our existing NIH-funded (P30 EY03039) Molecular Biology/Cellular Analysis Core grant module. The availability of the instruments will complement existing ERG capability and provide a powerful non-invasive analytical suite of instruments to analyze and correlate structure and function of the eye in vivo in the living animal. The proposed OCT/fundus imaging equipment bundle is planned to be housed in newly renovated space created to bring together researchers in Ophthalmology and Optometry and establish a strong cohesive group of retina researchers. The instruments are critical and essential new tools in five current NIH-funded projects (RO1 EY018143, Analysis of rod photoreceptor GARP and cGMP-gated cation channel in retina; RO1 EY019311, Rhodopsin Trafficking and Retinal Degenerations; RO1 EY006109, Quantitative Analysis of Aging Retina ; RO1 EY05922, Mechanisms of Ocular Development; and RO1 EY006400, Proteinase Inhibitors and Crystallin Fragments in Cataract). The OCT instrument will also be utilized by a group of minor users that are studying retina, optic nerve, heart and brain. Relevance: OCT is an emerging diagnostic imaging method enabling in vivo cross-sectional tomography of internal microstructures in living biological systems. While the instrumentation was first invented in the early 1990's, only recently has the technology evolved to allow ultra high resolution imaging that is approaching the quality and informational content of conventional invasive histologic analyses. No organ system is more amenable to OCT than the eye. OCT is now in routine use by Ophthalmologists and is becoming increasingly used in research in vision science. OCT is also being increasingly used in medical diagnostics in cardiology, gastroenterology, and other fields. While, routine histologic analysis is an end point experiment, OCT will allow the investigator to follow changes over time in the same animals. The proposed OCT and Retina Digital Imaging Equipment will enhance the already strong Vision Science Research Center Core Grant and adds a new dimension of analytical prowess to advance studies in many aspects of vision science and the study of other organ systems. Public Health Relevance: The instrumentation requested in this application will allow tissues to be visualized in living animals noninvasively. This is a monumental breakthrough in diagnostic capability that has been in use in the clinics for several years and is now available for large and small animals. The use of this new technology will dramatically advance our ability to correlate structure and function in living animals and in isolated tissues and will ultimately lead to new ways to treat diseases in humans.

描述（由申请人提供）：我们建议收购 Bioptigen, Inc. 840 nm SDOCT 光学相干断层扫描 (OCT) 与 Phoenix Research Laboratories, Inc. Micron II 视网膜成像显微镜套件（总成本 274,940 美元）捆绑在一起，用于人类和其他动物。这些仪器目前在 UAB 不可用，将为我们现有的 NIH 资助 (P30 EY03039) 分子生物学/细胞分析核心资助模块提供重要补充。这些仪器的推出将补充现有的 ERG 功能，并提供强大的非侵入性分析仪器套件，用于分析和关联活体动物体内眼睛的结构和功能。拟议的 OCT/眼底成像设备包计划安置在新装修的空间中，该空间旨在将眼科和验光研究人员聚集在一起，并建立一个具有强大凝聚力的视网膜研究人员团队。这些仪器是 NIH 目前资助的五个项目中关键且必不可少的新工具（RO1 EY018143，视网膜中视杆光感受器 GARP 和 cGMP 门控阳离子通道的分析；RO1 EY019311，视紫红质贩运和视网膜变性；RO1 EY006109，视网膜老化的定量分析；RO1 EY05922，眼部机制发展；和RO1 EY006400，白内障中的蛋白酶抑制剂和晶状体蛋白片段）。 OCT 仪器还将由一群研究视网膜、视神经、心脏和大脑的未成年用户使用。相关性：OCT 是一种新兴的诊断成像方法，能够对活体生物系统的内部微观结构进行体内横截面断层扫描。虽然该仪器于 20 世纪 90 年代初首次发明，但直到最近，该技术才发展到允许超高分辨率成像，其质量和信息内容已接近传统侵入性组织学分析的质量和信息内容。没有哪个器官系统比眼睛更适合 OCT。 OCT 现在已成为眼科医生的常规使用，并且越来越多地用于视觉科学研究。 OCT 也越来越多地应用于心脏病学、胃肠病学和其他领域的医疗诊断。虽然常规组织学分析是终点实验，但 OCT 将使研究人员能够跟踪同一动物随时间的变化。拟议的 OCT 和视网膜数字成像设备将增强本已强大的视觉科学研究中心核心拨款，并增加新的分析能力，以推进视觉科学许多方面的研究和其他器官系统的研究。 公共卫生相关性：本申请中要求的仪器将允许对活体动物的组织进行无创可视化。这是诊断能力的重大突破，已在诊所使用多年，现在可用于大型和小型动物。这项新技术的使用将极大地提高我们关联活体动物和分离组织的结构和功能的能力，并最终将带来治疗人类疾病的新方法。