Animal Model Core

动物模型核心

基本信息

批准号：
8150066
负责人：
Daniel Durant Myers
金额：
$ 23万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-04-01 至 2013-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8150066
关键词：
Adipocytes Allografting Animal Model Antibodies Anticoagulants Applications Grants Arts Blood Vessels Cardiovascular system Chemicals Complex Coronary Coronary Arteriosclerosis Disease Doctor of Medicine Doctor of Philosophy Equilibrium Fishes Funding Gene Mutation Genes Genomics Goals Head Hemostatic function House mice Human Human Genetics Image In Vitro Individual Inferior vena cava structure Internal Medicine Ligation Medical Genetics Medicine Methods Michigan Modeling Mus Operative Surgical Procedures Pharmacotherapy Physiological Plasminogen Activator Inhibitor 1 Principal Investigator Program Research Project Grants Research Research Personnel Risk Role Stenosis Survival Rate Testing Therapeutic Thrombosis Transplantation United States National Institutes of Health Universities Vascular Diseases Venous Venous Thrombosis Zebrafish high throughput screening in vitro testing in vivo lipid disorder lipid metabolism macrophage novel professor programs research study

项目摘要

Core A: Animal Model Core The purpose of the Animal Model Core A is to provide each investigator of the PPG a wide array of animal models to assess lipid metabolism, thrombosis, and related vascular disorders. Project 1) Daniel A. Lawrence, Ph.D., Professor of Cardiovascular Medicine, Department of Internal Medicine: Characterization of a Novel Role for PAI-1 in Lipid Metabolism. Core A will provide surgical support of Dr. Lawrence's in vivo experiments as outlined in this grant application. Core A will also be responsible for the cellular isolation of murine macrophages and adipocytes for in vitro experiments outlined in Project 1 for experiments evaluating the role of PAI-1 in lipid metabolism for Dr. Lawrence. Project 2) Thomas W. Wakefield, M.D., Head, Vascular Surgery Section, Department of Surgery: Role of PAI-1 in Venous Thrombogenesis. Core A will provide three animal models of venous thrombosis for in vitro testing for Project 2. These animal models include the following: 1) Inferior Vena Cava (IVC) Stenosis Model of Venous Thrombosis and 2) IVC ligation Model of Venous Thrombosis. Core A will perform all surgical procedures for experiments outlined by Dr. Wakefield and Dr. Peter K. Henke in Project 2. Project 3) David J. Pinsky, M.D., Chief, Cardiovascular Medicine, Department of Internal Medicine: Thrombotic/fibrinolytic Balance in Coronary Transplant Vasculopathy. Animal Model Core A will provide surgical support of Dr. Pinsky's murine in vivo experiments as outlined in this grant application. Core A will facilitate the acquisition of mouse echocardiograph images in the proposed transplant associated coronary artery disease (TCAD) experiments. Animal Model Core A will be responsible for both the preoperative administration of antibodies to allograft recipients and the daily assessment of mouse allograft to determine survival rates. Project 4) David Ginsburg, M.D., Division of Medical Genetics, Department of Internal Medicine and Department of Human Genetics: Identifying Thrombosis Modifier Genes and Novel Anticoagulants in Zebra fish. Core A will directly collaborate with Dr. Ginsburg (Project 4) and coordinate specific Core A interactions with Co-Pi Dr. David Sherman of the Center for Chemical Genomics (CCG) to develop high throughput chemical screens to identify novel anticoagulants in zebrafish (Danio rerio) These screens will focus on lipid disorders and vascular disease with the goal of providing novel drug therapies to treat human disorders of hemostasis. It is intended that Core A will be a central part of the PPG Program and will benefit all investigators involved.

核心A：动物模型核心动物模型核心A的目的是为PPG的每个研究者提供各种动物评估脂质代谢，血栓形成和相关血管疾病的模型。项目1）Daniel A. Lawrence博士，内部心血管医学教授医学：PAI-1在脂质代谢中的新作用的表征。核心A将提供手术该赠款申请中概述了劳伦斯博士的体内实验。核心也将是负责详细概述的体外实验的鼠巨噬细胞和脂肪细胞的细胞分离在项目1中，用于评估PAI-1在Lawrence博士脂质代谢中的作用的实验。项目2）托马斯·W·韦克菲尔德（Thomas W. Wakefield） PAI-1在静脉血栓形成中的作用。核心A将提供三种静脉血栓形成的动物模型对于项目2的体外测试。这些动物模型包括：1）下腔静脉（IVC）静脉血栓形成的狭窄模型和2）静脉血栓形成的IVC连接模型。核心遗嘱在项目中概述了Wakefield博士和Peter K. Henke博士概述的所有外科手术程序 2。项目3）内科医学系心血管医学首席医学博士David J. Pinsky：冠状动脉移植血管病中的血栓性/纤维蛋白水解平衡。动物模型核心A遗嘱如本赠款申请中概述的那样，请提供Pinsky博士在体内实验的手术支持。核 A将有助于在拟议的移植相关的移植中获取鼠标超声心动图图像冠状动脉疾病（TCAD）实验。动物模型核心A将负责术前施用对同种异体移植受体的抗体和小鼠同种异体的每日评估确定生存率。项目4）医学博士David Ginsburg，内科医学系医学遗传学部人类遗传学系：鉴定血栓形成修饰剂基因和新型抗凝剂斑马鱼。核心A将直接与金斯堡博士（项目4）合作，并协调特定的核心A 与化学基因组中心（CCG）的Co-Pi互动，以发展高吞噬化学筛选以鉴定斑马鱼（Danio rerio）中的新型抗凝剂这些筛选将这些筛选专注于脂质疾病和血管疾病，目的是提供新的药物疗法来治疗人类止血疾病。旨在使核心A将成为PPG计划的中心部分，并将使所有相关研究人员受益。