Animal Model Core

动物模型核心

• 批准号: 8150066
• 负责人: Daniel Durant Myers
• 金额: $ 23万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8150066
• 关键词: Adipocytes; Allografting; Animal Model; Antibodies; Anticoagulants; Applications Grants; Arts; Blood Vessels; Cardiovascular system; Chemicals; Complex; Coronary; Coronary Arteriosclerosis; Disease; Doctor of Medicine; Doctor of Philosophy; Equilibrium; Fishes; Funding; Gene Mutation; Genes; Genomics; Goals; Head; Hemostatic function; House mice; Human; Human Genetics; Image; In Vitro; Individual; Inferior vena cava structure; Internal Medicine; Ligation; Medical Genetics; Medicine; Methods; Michigan; Modeling; Mus; Operative Surgical Procedures; Pharmacotherapy; Physiological; Plasminogen Activator Inhibitor 1; Principal Investigator; Program Research Project Grants; Research; Research Personnel; Risk; Role; Stenosis; Survival Rate; Testing; Therapeutic; Thrombosis; Transplantation; United States National Institutes of Health; Universities; Vascular Diseases; Venous; Venous Thrombosis; Zebrafish; high throughput screening; in vitro testing; in vivo; lipid disorder; lipid metabolism; macrophage; novel; professor; programs; research study

Core A: Animal Model Core The purpose of the Animal Model Core A is to provide each investigator of the PPG a wide array of animal models to assess lipid metabolism, thrombosis, and related vascular disorders. Project 1) Daniel A. Lawrence, Ph.D., Professor of Cardiovascular Medicine, Department of Internal Medicine: Characterization of a Novel Role for PAI-1 in Lipid Metabolism. Core A will provide surgical support of Dr. Lawrence's in vivo experiments as outlined in this grant application. Core A will also be responsible for the cellular isolation of murine macrophages and adipocytes for in vitro experiments outlined in Project 1 for experiments evaluating the role of PAI-1 in lipid metabolism for Dr. Lawrence. Project 2) Thomas W. Wakefield, M.D., Head, Vascular Surgery Section, Department of Surgery: Role of PAI-1 in Venous Thrombogenesis. Core A will provide three animal models of venous thrombosis for in vitro testing for Project 2. These animal models include the following: 1) Inferior Vena Cava (IVC) Stenosis Model of Venous Thrombosis and 2) IVC ligation Model of Venous Thrombosis. Core A will perform all surgical procedures for experiments outlined by Dr. Wakefield and Dr. Peter K. Henke in Project 2. Project 3) David J. Pinsky, M.D., Chief, Cardiovascular Medicine, Department of Internal Medicine: Thrombotic/fibrinolytic Balance in Coronary Transplant Vasculopathy. Animal Model Core A will provide surgical support of Dr. Pinsky's murine in vivo experiments as outlined in this grant application. Core A will facilitate the acquisition of mouse echocardiograph images in the proposed transplant associated coronary artery disease (TCAD) experiments. Animal Model Core A will be responsible for both the preoperative administration of antibodies to allograft recipients and the daily assessment of mouse allograft to determine survival rates. Project 4) David Ginsburg, M.D., Division of Medical Genetics, Department of Internal Medicine and Department of Human Genetics: Identifying Thrombosis Modifier Genes and Novel Anticoagulants in Zebra fish. Core A will directly collaborate with Dr. Ginsburg (Project 4) and coordinate specific Core A interactions with Co-Pi Dr. David Sherman of the Center for Chemical Genomics (CCG) to develop high throughput chemical screens to identify novel anticoagulants in zebrafish (Danio rerio) These screens will focus on lipid disorders and vascular disease with the goal of providing novel drug therapies to treat human disorders of hemostasis. It is intended that Core A will be a central part of the PPG Program and will benefit all investigators involved.

核心A：动物模型核心 动物模型核心A的目的是为PPG的每个调查者提供广泛的动物 评估脂代谢、血栓形成和相关血管疾病的模型。 项目1)丹尼尔·A·劳伦斯博士，内科心血管内科教授 医学：PAI-1在脂质代谢中的新作用的特征。核心A将提供外科手术 支持劳伦斯博士的活体实验，如本拨款申请中所述。核心A也将是 负责小鼠巨噬细胞和脂肪细胞的细胞分离 在项目1中，为劳伦斯博士评估PAI-1在脂质代谢中的作用。 项目2)Thomas W.Wakefield，医学博士，外科血管外科科长： 纤溶酶原激活物-1在静脉血栓形成中的作用Core A将提供三种静脉血栓形成的动物模型 这些动物模型包括：1)下腔静脉(IVC) 静脉血栓狭窄模型和下腔静脉结扎血栓模型。核心A将 为韦克菲尔德博士和彼得·K·亨克博士在项目中概述的实验执行所有手术程序 2. 项目3)David J.Pinsky，医学博士，内科心血管内科主任： 冠状动脉移植血管病变中的血栓/纤溶平衡。动物模型核心A意志 为平斯基博士的小鼠活体实验提供手术支持，如本拨款申请中所述。堆芯 将便于获取小鼠超声心动图图像的建议移植相关 冠状动脉疾病(TCAD)实验。动物模型核心A将负责这两项 同种异体移植受者的术前抗体应用及小鼠移植后的日常评估 以确定存活率。 项目4)David Ginsburg，医学博士，医学遗传学，内科和 人类遗传学系：鉴定血栓形成调节剂基因和新型抗凝剂 斑马鱼。核心A将直接与金斯伯格博士合作(项目4)，并协调具体的核心A 与化学基因组学中心(CCG)的共同PI博士David Sherman的互动开发高 通过化学筛选鉴定斑马鱼(Danio Rerio)中的新型抗凝血剂这些筛选将 专注于血脂紊乱和血管疾病，目标是提供治疗人类 止血障碍。 其目的是，核心A将成为PPG计划的核心部分，并将使所有参与的调查人员受益。