Imaging Hyaloid Regression

描述(申请人提供)：在晶状体的形成过程中，形成一个短暂的血管网络，即玻璃体网络，在晶状体发育过程中滋养晶状体。然而，在人类胎儿发育晚期和小鼠出生后早期发育过程中，玻璃体血管经历了倒退，从而能够通过晶状体获得透明的视觉路径。玻璃体血管的异常消退或生长可导致多种疾病，包括持续性血管膜增生性晶状体(PHTVL)、持续性初级玻璃体增生症(PHPV)、持续性胎儿血管(PFV)和持续性瞳孔前膜(PPM)。在这项研究中，我们将使用荧光报告鼠标记涉及血管退化的关键细胞类型、实时共聚焦成像和FACS分类和表达分析，以确定导致血管退化开始的细胞事件。 与公共卫生相关：眼内血管的异常生长和退化是导致儿童、成人和老年人失明的主要原因(Campochiaro，2000)。这项研究将使用重要的成像来表征新生血管是如何退化的，使用小鼠模型，在小鼠模型中，玻璃体血管中的单个细胞类型被荧光蛋白记者标记。将使用实时成像和FACS分类来定义启动血管回归中的新事件。

DESCRIPTION (provided by applicant): During the formation of the lens, a transient vessel network, the hyaloid network, forms to nourish the lens as it develops. However, during late fetal development in humans and early postnatal development in mice, hyaloid vessels undergo regression to enable a transparent path of vision through the lens. Abnormal regression or growth of the hyaloid vasculature results in several diseases, including persistent hyperplastic tunica vasculosa lentis (PHTVL), persistent hyperplastic primary vitreous (PHPV), persistent fetal vasculature (PFV) and persistent prepupillary membrane (PPM). In this study, we will use fluorescent reporter mice labeling key cell types involved in vessel regression, live confocal imaging and FACS sorting and expression analysis to define cellular events leading to the onset of vessel regression. PUBLIC HEALTH RELEVANCE: The abnormal growth and regression of blood vessels in the eye is the leading cause of blindness in children, adults and the elderly (Campochiaro, 2000). This study will use vital imaging to characterize how vessels regress in the newborn eye using mouse models in which individual cell types in the hyaloid vasculature are labeled with fluorescent protein reporters. Live imaging and FACS sorting will be used to define novel events in that initiate vessel regression.