Thermo-detachable anti-biofilm polymer coatings

热可拆卸抗生物膜聚合物涂层

• 批准号: 8059667
• 负责人: Kenichi Kuroda
• 金额: $ 19.12万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8059667
• 关键词: Adverse effects; Alder plant; Bacteria; Biological Models; Cell surface; Chemicals; Chemistry; Cleaved cell; Clinical; Defense Mechanisms; Dental; Dental Care; Diels Alder reaction; Equipment; Erythrocytes; Excision; Flushing; Furans; Gastrointestinal tract structure; Growth; Heating; High temperature of physical object; Hot flushes; Infection Control; Intestines; Long-Term Effects; Maleimides; Medical; Medical Device; Medicine; Membrane; Methods; Microbial Biofilms; Modification; Mucous Membrane; Output; Polymers; Prevention; Procedures; Process; Public Facilities; Public Health; Research; Steam; Surface; System; Temperature; Testing; Time; Uncertainty; Water; Work; adduct; antimicrobial; base; cold temperature; cost effective; covalent bond; design; innovation; instrument; killings; medical implant; microorganism; novel; novel strategies; prevent; public health relevance; success; vapor; water quality; water treatment

DESCRIPTION (provided by applicant): Preventing biofilm formation and removing existing biofilms effectively from dental unit water lines (DUWLs) are crucial to maintaining the quality of dental treatment water for infection control. Several physical and chemical approaches and automatic cleaning systems have been developed to control biofilm formation and output water quality in DUWLs; however, the long-term effect of these approaches is still in doubt, and adverse effects are found to be associated with some of these approaches. Therefore, there is a great need to develop novel approaches for preventing biofilm formation and removing resident biofilms in order to achieve highly effective control of biofilms and output water quality in DUWLs. In this study, a new design concept, inspired by the defense mechanisms employed by our own body against harmful microorganisms in the gastrointestinal tract, is proposed to create material surfaces modified with polymeric materials that have dual functionalities to control biofilm formation as well as to facilitate removal of biofilms. The central hypothesis is that biofilm formation and removal can be effectively controlled by on-demand detachment of antimicrobial and thermo- detachable polymer coatings. Antimicrobial polymers will be coated on interior surfaces of water tubing by thermally cleavable linkers between the coating polymers and surface. The linkers are based on the thermally reversible Diels-Alder chemistry: the covalent bond is formed at room temperature to 600C and cleaved at higher temperatures (> 900C), allowing annealing the coatings to anchor the polymers onto surfaces at low temperatures and removing the coatings using hot water or steam vapor. The polymers will prevent or delay biofilm formation by killing bacteria adhered to the surface. Once biofilms are formed after extended periods of use, heating the coating by hot water or steam vapor cleaves the linkages, and the coatings will be detached from the surface. The biofilms on the coatings will be flushed out together with the detached polymers. After biofilm removal, the clean surface will be coated again by the thermally detachable polymers, and the process can be repeated multiple times. To test the hypothesis, two specific aims are proposed: (1) to develop the thermo-detachable antimicrobial coatings and (2) to evaluate biofilm growth and biofilm removal on the polymer coatings. This new coating design is innovative because these coatings will prevent or delay biofilm formation as well as remove formed biofilms efficiently, and this design will allow cleaning inner surfaces of tubing and connectors of DUWLs by flushing hot water/steam vapor, which can be completed without opening tubing systems and the use of chemicals. Recoating the interior surfaces with our polymers will also offer a fresh antimicrobial and detachable coating, which will extend the lifetime of DUWLs substantially. This novel design concept with necessary modifications would also find potential applications in other medical and industrial systems such as medical devices and implants, dental treatment instruments, water-cooling towers, and municipal water systems, where biofilm formation are of significant concern and difficult to remove.

描述（由申请人提供）：防止生物膜形成并有效去除牙科综合治疗台水管（DUWL）中现有的生物膜对于保持牙科治疗用水的质量以控制感染至关重要。已经开发了几种物理和化学方法以及自动清洗系统来控制DUWL中的生物膜形成和输出水质;然而，这些方法的长期效果仍然值得怀疑，并且发现与其中一些方法相关的不利影响。因此，非常需要开发用于防止生物膜形成和去除驻留生物膜的新方法，以实现对DUWL中的生物膜和输出水质的高效控制。在这项研究中，一个新的设计概念，灵感来自我们自己的身体对胃肠道中的有害微生物的防御机制，提出了创建具有双重功能的聚合物材料改性的材料表面，以控制生物膜的形成，以及促进生物膜的去除。中心假设是生物膜的形成和去除可以通过抗微生物和热可分离聚合物涂层的按需分离来有效控制。抗菌聚合物将通过涂层聚合物和表面之间的可热裂解的连接剂涂覆在水管的内表面上。该连接剂基于热可逆的狄尔斯-阿尔德化学：共价键在室温至600 ℃下形成，并在较高温度（> 900 ℃）下裂解，允许在低温下对涂层进行退火以将聚合物锚在表面上，并使用热水或蒸汽去除涂层。聚合物将通过杀死粘附在表面上的细菌来防止或延迟生物膜的形成。一旦在长时间使用后形成生物膜，通过热水或蒸汽加热涂层会使连接断裂，并且涂层将从表面分离。涂层上的生物膜将与分离的聚合物一起被冲洗掉。在生物膜去除之后，清洁表面将再次被可热分离的聚合物涂覆，并且该过程可以重复多次。为了验证这一假设，提出了两个具体目标：（1）开发热可分离的抗菌涂层和（2）评价聚合物涂层上的生物膜生长和生物膜去除。这种新的涂层设计是创新的，因为这些涂层将防止或延迟生物膜形成以及有效地去除形成的生物膜，并且这种设计将允许通过冲洗热水/蒸汽来清洁DUWL的管道和连接器的内表面，这可以在不打开管道系统和使用化学品的情况下完成。用我们的聚合物覆盖内表面还将提供新的抗菌和可拆卸涂层，这将大大延长DUWL的使用寿命。这种新颖的设计概念与必要的修改，也将发现在其他医疗和工业系统，如医疗设备和植入物，牙科治疗仪器，水冷却塔，和市政供水系统，其中生物膜的形成是显着的关注和难以去除的潜在应用。

项目成果

Immobilization of amphiphilic polycations by catechol functionality for antimicrobial coatings.

抗微生物涂层的儿茶酚功能将两亲和固定的固定。

• DOI: 10.1021/la1046904
• 发表时间: 2011-04-05
• 期刊: Langmuir : the ACS journal of surfaces and colloids
• 作者: Han H;Wu J;Avery CW;Mizutani M;Jiang X;Kamigaito M;Chen Z;Xi C;Kuroda K
• 通讯作者: Kuroda K

Utilization of star-shaped polymer architecture in the creation of high-density polymer brush coatings for the prevention of platelet and bacteria adhesion.

• DOI: 10.1039/c4bm00034j
• 发表时间: 2014-09-01
• 期刊: Biomaterials science
• 影响因子: 6.6
• 作者: Totani M;Ando T;Terada K;Terashima T;Kim IY;Ohtsuki C;Xi C;Kuroda K;Tanihara M
• 通讯作者: Tanihara M

Catechol-Functionalized Synthetic Polymer as a Dental Adhesive to Contaminated Dentin Surface for a Composite Restoration.

• DOI: 10.1021/acs.biomac.5b00451
• 发表时间: 2015-08-10
• 期刊: Biomacromolecules
• 影响因子: 6.2
• 作者: Lee SB;González-Cabezas C;Kim KM;Kim KN;Kuroda K
• 通讯作者: Kuroda K

Poly(ethylene imine)s as antimicrobial agents with selective activity.

• DOI: 10.1002/mabi.201200052
• 发表时间: 2012-09
• 期刊: MACROMOLECULAR BIOSCIENCE
• 影响因子: 4.6
• 作者: Gibney, Katherine A.;Sovadinova, Iva;Lopez, Analette I.;Urban, Michael;Ridgway, Zachary;Caputo, Gregory A.;Kuroda, Kenichi
• 通讯作者: Kuroda, Kenichi