Monkey Cortical Connections Database

描述（由申请人提供）：灵长类动物大脑皮层连通性的知识对于理解感觉、知觉和认知的高级大脑机制及其障碍至关重要，但连接追踪研究越来越不常见，较早的研究只能通过代表早期研究者对数据的解释的出版物获得，并且通常无法获得原始数据进行重新解释。检索实验材料档案并将其转换为可访问的数字形式存在相当大的后勤和其他障碍。我们试图建立一个全面的、持续的材料档案，展示恒河猴大脑皮层和皮层下的连通性，其中所有来自个体实验的组织学材料都以数字形式提供，并提供合适的搜索引擎，向所有研究者开放访问。通过这种方式，原始的实验数据可以被注释和重新解释，因为在积累功能和临床数据的基础上对皮层和皮层下结构进行了更新的分割。目前的提议建立在构建灵长类大脑结构的高分辨率显微地图集的专业知识之上，并将创建一个成年恒河猴大脑皮层连接的可搜索数据库。将建立一个管道，在一系列猴子的目标皮质区域注射逆行和顺行运输的示踪剂。每个个体大脑的数字数据将通过以下方式收集：结构mri；在脑切片过程中，每40<m切片被切除时，所切片的黑脸的连续成像；高分辨率脑交替切片成像，用运输示踪剂染色或尼氏染色；将各部分的图像扭曲回大脑和核磁共振成像的面部图像上，为每个大脑提供三维体积数据集。存储单个数据集的可搜索数据库将包含一种能力，可以根据标准的高分辨率恒河猴大脑数字图谱对来自每个实验大脑的数据集进行注释，一种能力，可以通过名称或通过视觉检查注射区域或可能的传入源和传入目标来查询连接，一种与其他相关神经科学信息数据库互操作的能力。该数据库还将允许纳入从出版物和/或现有幻灯片中提取的连接数据集。其结果将是一个活生生的档案，其中对理解神经和神经精神疾病的病理生理学至关重要的数据可以继续添加和查询。了解灵长类动物大脑皮层的功能区域如何相互连接，对于理解感觉、感知和认知的大脑机制及其障碍，特别是与功能性脑成像有关的机制至关重要。然而，相关的实验数据正变得难以获得，因为正在进行的研究很少，在较旧的出版物形式中不提供获取原始数据的途径，并且没有此类信息的档案。我们试图建立一个全面的和持续的材料档案，在微观分辨率下展示恒河猴大脑皮层和皮层下的连通性。在该数据库中，所有来自个体实验的材料都以数字形式提供，并有一个合适的搜索引擎向所有研究人员开放访问，并与其他神经科学信息数据库相互关联。其结果将是一个活生生的档案，其中对理解神经和神经精神疾病的病理生理学至关重要的数据可以继续添加和查询。

DESCRIPTION (provided by applicant): Knowledge of cerebral cortical connectivity in primates is essential for understanding higher brain mechanisms of sensation, perception and cognition and their disorders but connection tracing studies are increasingly uncommon, older studies are accessible only through publications which represent an earlier investigator's interpretation of the data, and access to the original data for re-interpretation is commonly unavailable. Retrieval of archives of experimental material and conversion to an accessible digital form presents considerable logistical and other handicaps. We seek to develop a comprehensive and ongoing archive of material demonstrating cortical and subcortical connectivity in rhesus monkey brains, in which all the histological material from individual experiments is available in its digital form, with a suitable search engine providing open access to all investigators. In this way, original experimental data can be annotated and re-interpreted as newer parcellations of cortical and subcortical structures are made on the basis of accumulating functional and clinical data. The current proposal builds on expertise in constructing high resolution microscopic atlases of primate brain architecture and will create a searchable database of connections of the cerebral cortex in the adult rhesus monkey. A pipeline will be established in which targeted cortical areas in a series of monkeys will be injected with a tracer transported both retrogradely and anterogradely. Digital data about each individual brain will be collected by means of: structural mri; serial imaging of the sectioned blockface as each 40<m section is removed during sectioning of the brain; imaging at high resolution of alternate sections through the brain stained for the transported tracer or stained with the nissl stain; warping images of the sections back onto the blockface images of the brain and mris to provide a 3d volumetric dataset for each brain. The searchable database into which the individual datasets will be stored will incorporate a capacity to annotate the dataset from each experimental brain in relation to a standard high resolution digital atlas of the rhesus monkey brain, a capacity for making queries about connections by name or by visual inspection of injected regions or of likely sources of afferents and targets of efferents, a capacity for interoperability with databases of other relevant neuroscience information. The database will also permit incorporation of datasets of connectivity drawn from material in publications and/or existing slide. The result will be a living archive to which data essential for the understanding of the pathophysiology of neurological and neuropsychiatric disorders can continue to be added and queried. PUBLIC HEALTH RELEVANCE Knowledge of how functional regions of the primate cerebral cortex are connected with one another is essential for understanding brain mechanisms of sensation, perception and cognition and their disorders, especially in relation to functional brain imaging. Relevant experimental data however is becoming hard to obtain because few studies are being done, access to original data is not provided in older forms of publication and theire are no archives of such information. We seek to develop a comprehensive and ongoing archive of material demonstrating cortical and subcortical connectivity in rhesus monkey brains at microscopic resolution. In it, all the material from individual experiments is available in digital form, with a suitable search engine providing open access to all investigators and interrelated with other databases of neuroscientific information. The result will be a living archive to which data essential for the understanding of the pathophysiology of neurological and neuropsychiatric disorders can continue to be added and queried.