Pulvinar cellular and network dynamics in cognitive control of sensory processes.

项目1总结 P1与中心的3个首要目标中的每一个保持一致：1.确定认知功能组织原则 丘脑高级核团与皮质的相互作用；2.从动物模型中翻译丘脑功能 为健康和患病的人脑；3.建立一个生物学上可信的人类高级丘脑模型。 具体地说，P1将研究与早期视觉皮质区域(V1、V2和V2)相关的枕骨区域 V4)检验关于高级丘脑的认知和电路动力学的具体假设。这个项目需要 枕骨和皮质之间的视网膜定位关系的优势使电极的精确放置成为可能 在突触、细胞和网络水平上研究认知控制对电路动力学的影响。在……里面 与中心目标1对齐，将P1的结果与P2、P3和P4的结果进行比较，以建立 全面和机械性地理解高级丘脑在认知控制中的作用。在……里面 与中心目标2保持一致，来自P1的结果将为理解疾病的影响提供一个框架 In(P5)。为了与中心目标3保持一致，P1的结果将随着其模型的发展而通知核心B。核心C将 为P1提供必要的分析工具，并确保数据可以在 项目。 在P1/AIM 1中，我们将记录与V1、V2和V2相关的光遗传学识别的枕骨区域 V4在执行APU任务的猴子中。然后我们将光基因沉默这些PUL区域的V1输入 评估他们在决策和空间注意力方面的作用。在P1/AIM 2中，我们将使用我们的 实验室，“双神经元休克-尖峰实验”，以确定注意效应的空间组织。 枕叶和注意对枕叶-皮质沟通效果的影响。最后，在P1/AIM 3中，我们 将利用光遗传学来灭活枕骨内的V1和V2轴突，以确定认知能力的影响 影响脑枕皮质-皮质通讯功能的因素。

PROJECT 1 SUMMARY P1 is aligned with each of the Center’s 3 overarching aims: 1. Identify cognitive functional organization principles of higher order thalamic nuclei in interacting with cortex; 2. Translate thalamic functionality from animal models to healthy and diseased human brain; 3. Develop a biologically plausible model for human higher order thalamus. Specifically, P1 will investigate regions of the pulvinar associated with early visual cortical areas (V1, V2, and V4) to test specific hypotheses about cognition and circuit dynamics in higher order thalamus. This project takes advantage of the retinotopic relationship between pulvinar and cortex to enable precise electrode placement for studying the influence of cognitive control on circuit dynamics at a synaptic, cellular, and network level. In alignment with Center Aim 1, results from P1 will be compared to those from P2, P3, and P4 to establish a comprehensive and mechanistic understanding of the role of higher order thalamus in cognitive control. In alignment with Center Aim 2, results from P1 will provide a framework for understanding the impact of disease in (P5). In alignment with Center Aim 3, results from P1 will inform Core B as its models evolve. Core C will provide essential analytical tools for P1 as well as ensure that data can be exchanged and compared between projects. In P1/Aim 1, we will record from optogenetically identified regions of pulvinar associated with V1, V2, and V4 in monkeys performing the APU task. We will then optogenetically silence the V1 inputs to these PUL regions to assess their role in decision making and spatial attention. In P1/Aim 2, we will use an assay developed in our laboratory, the “dual-neuron shock-spike assay”, to determine the spatial organization of attention effects in the pulvinar and the influence of attention on the efficacy of pulvino-cortical communication. Lastly, in P1/Aim 3, we will use optogenetics to inactivate V1 and V2 axons within the pulvinar to determine the influence of cognitive factors on pulvinar regulation of cortico-cortical communication.