Effect of antimicrobial nanoemulsions on oral biofilms

抗菌纳米乳对口腔生物膜的影响

• 批准号: 8073461
• 负责人: VALERIE A LEE
• 金额: $ 12.64万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2013-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8073461
• 关键词: Adult; Affect; Animals; Antimicrobial Effect; Award; Bacteria; Biocompatible Materials; Cell Death; Cell membrane; Charge; Communities; Complex; Coupled; Dental Hygiene; Dental Plaque; Dental caries; Diet; Drug Formulations; Ecology; Edetic Acid; Effectiveness; Emulsions; Environment; Experimental Designs; Exposure to; Fluorescent in Situ Hybridization; General Population; Goals; Growth; Health Professional; In Vitro; Incidence; Investigation; Iron; Laboratories; Lactoferrin; Lasers; Mediating; Mentors; Methods; Microbial Biofilms; Microbiology; Molecular Biology; Oils; Oral; Oral cavity; Oral health; Organism; Osmolar Concentration; Oxygen; Phase; Physiological; Population; Predisposition; Prevention; Rattus; Reproduction spores; Research; Research Personnel; Resistance; Salts; Scanning; Scientist; Series; Streptococcus mutans; Structure; Sucrose; Surface; Techniques; Technology; Testing; Texas; Tooth Diseases; Toxic effect; Universities; Virus; Water; Work; antimicrobial; antimicrobial drug; base; career; fungus; innovation; microorganism; nanoDroplet; nanoemulsion; oral biofilm; permanent tooth; prevent; programs; research study; success; surfactant; tooth surface

DESCRIPTION (provided by applicant): The broad objective of the proposed work is to determine optimum nanoemulsion formulations that effectively disrupt or prevent the formation and growth of oral biofilms associated with dental caries, and also to understand the mechanisms involved in their disruption. The incidence of dental caries in the population is still high. The average adult in the U.S. has from 10 to 17 decayed, missing or filled permanent teeth. Nanoemulsions are surfactant-containing oil-in-water emulsions (droplet size 400-800 nm) that are non-toxic to animals but very effective against bacteria, fungi, viruses and spores in their free-floating form. Specific Aim 1: Determine optimum nanoemulsion formulations that prevent the formation and growth of, and disrupt the presence of mixed-organism biofilms in vitro. Specific Aim 2: Investigate the effectiveness of selected nanoemulsions in a study involving rats. Specific Aim 3: Evaluate the in vitro susceptibility of biofilms to nanoemulsions when biofilms are grown under varying physiological conditions likely to be present in the oral cavity (high-low sucrose, high-low iron, high-low osmolarity, high-low oxygen), and also investigate changes in biofilm structure and community profiles. Both S. mutans in pure culture and defined mixed-organism cultures will be investigated. Specific Aim 4: Determine the mechanism of action of the nanoemulsion effect on S. mutans. The proposed work is innovative because the effects of antimicrobial nanoemulsions on biofilms common to the oral cavity have not been investigated. My goal is to be an academic biomaterials scientist with an independent research effort in the treatment and prevention of intra-oral biofilms, and the investigation of mechanisms involved in their disruption. I have successfully found expert collaborators at UTHSCSA and Texas State University for this interdisciplinary project. A K08 award will allow me to acquire the necessary microbiology and molecular biology background and laboratory techniques to successfully complete the proposed work, and will also allow me to utilize the benefits of mentoring during the transition to an independent research career. This research studies a new method of preventing tooth decay. Success may alleviate this problem, which affects a majority of people.

描述（由申请人提供）：拟议工作的广泛目标是确定有效破坏或防止与龋齿相关的口服生物膜的形成和生长的最佳纳米乳液配方，并了解其干扰所涉及的机制。人口中龋齿的发生率仍然很高。美国的平均成年人从10到17个腐烂，缺失或填充的恒牙。纳米乳剂是含表面活性剂的含油含油乳液（液滴尺寸为400-800 nm），它们对动物无毒，但对细菌，真菌，病毒和孢子的自由浮动形式非常有效。具体目标1：确定最佳的纳米乳液制剂，以防止在体外形成和生长，并破坏体外混合生物生物膜的存在。具体目标2：研究选定的纳米乳液在涉及大鼠的研究中的有效性。具体目的3：当生物膜在不同的生理条件下生长时，生物膜对纳米乳液的体外敏感性可能存在于口腔中（高低蔗糖，高低铁，高低渗透压，高低氧气），并且还研究了生物膜结构和社区profiles和社区profiles的变化。将研究纯文化中的链球菌和定义的混合生物培养物。特定目的4：确定纳米乳剂对链球菌的作用机理。拟议的工作具有创新性，因为尚未研究抗菌纳米乳液对口腔共有的生物膜的影响。我的目标是成为一名学术生物材料科学家，并在治疗和预防口腔内生物膜方面进行独立的研究工作，并研究涉及破坏的机制。我成功地在UTHSCSA和德克萨斯州立大学找到了这个跨学科项目的专家合作者。 K08奖将使我能够获得必要的微生物学和分子生物学背景和实验室技术，以成功完成拟议的工作，还可以使我在过渡到独立研究职业的过程中利用指导的好处。该研究研究了一种预防蛀牙的新方法。成功可能会减轻这一问题，这会影响大多数人。