ALOGLIPTIN EFFECTS ON PANCREATIC ISLET FUNCTION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目的目标是评估DPP-4抑制剂对胰岛活性和功能的影响，特别是对基础和GLP-1增强的葡萄糖刺激的胰岛素分泌的影响。这一建议是基于观察到DPP-4抑制增强体内胰岛功能和胰腺阿尔法细胞表达DPP-4，支持DPP-4抑制剂可能具有胰岛自主效应的假说，这将在体外分离的胰岛中可察觉到。到目前为止，我们已经建立了猕猴胰岛纯化和静态葡萄糖刺激的胰岛素分泌程序的优化方案。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project has as its goal the evaluation of the effects of a DPP-4 inhibitor on pancreatic islet viability and function, specifically with respect to basal and GLP-1-enhanced glucose-stimulated insulin secretion. The proposal is based upon the observation that DPP-4 inhibition increases islet function in vivo and that pancreatic alpha cells express DPP-4, supporting the hypothesis that DPP-4 inhibitors may have islet-autonomous effects that will be discernable in isolated islets ex vivo. To date, we have established an optimized protocol for macaque islet purification and procedures for static glucose-stimulated insulin secretion.