ALOGLIPTIN EFFECTS ON PANCREATIC ISLET FUNCTION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本项目的目标是评价DPP-4抑制剂对胰岛活力和功能的影响，特别是在基础和GLP-1增强的葡萄糖刺激胰岛素分泌方面。 该提议基于以下观察结果：DPP-4抑制可增加体内胰岛功能，胰腺α细胞表达DPP-4，支持DPP-4抑制剂可能具有在离体胰岛中可辨别的胰岛自主效应的假设。 到目前为止，我们已经建立了一个优化的协议猕猴胰岛纯化和程序静态葡萄糖刺激的胰岛素分泌。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project has as its goal the evaluation of the effects of a DPP-4 inhibitor on pancreatic islet viability and function, specifically with respect to basal and GLP-1-enhanced glucose-stimulated insulin secretion. The proposal is based upon the observation that DPP-4 inhibition increases islet function in vivo and that pancreatic alpha cells express DPP-4, supporting the hypothesis that DPP-4 inhibitors may have islet-autonomous effects that will be discernable in isolated islets ex vivo. To date, we have established an optimized protocol for macaque islet purification and procedures for static glucose-stimulated insulin secretion.