Core--Centralized Facility/Mouse

鼠标核心的主要目标是提供一个集中的面向服务的设施，以确保 每个参与项目的实验室生产和维持转基因和基因破坏小鼠的能力。的 该设施于1995年在肺科开发。最初，该设施是用资金装备的， 巴恩斯犹太医院基金会，并在历史上主要服务于实验室涉及肺 research.最近，该设施主要得到了两项NIH赠款的支持，其中包括SCOR赠款， 哮喘（气道炎症和重塑的机制，P50 HL 56419，Michael J. Holtzman，PI），和 计划项目资助（肺结构重塑的机制，P01 HL 29594，罗伯特M。前辈， PI）。由于本申请中的大多数项目都是在小鼠中进行遗传实验，因此我们建议这笔拨款 还支持鼠标核心的比例部分。许多项目负责人、合作研究者和核心研究人员 该应用程序的领导者（Holtzman，Mecham，Pierce，Senior，Walter，Urban，Atkinson，Brody，Shipley） 几年来，我一直在使用它来进行与这两项赠款以及其他项目有关的工作。自1995年以来， 该设施已经产生了100多个系的转基因和基因靶向小鼠，多个创始人或 在大多数情况下，每一条线的嵌合体。这通过最大限度地减少开销和成本来实现规模经济， 避免重复需要专门设备和动物设施的高技术技能。鼠标 核心是合作的主要途径。执行功能获得和功能丧失的能力 体内实验已经并将增强我们对控制肿瘤生长的分子机制的理解。 慢性阻塞性肺病的肺重塑此外，该核心还有助于开发一种小鼠模型， 吸烟引起的肺气肿在此应用程序中，核心还将支持此 模型，因为它将被大多数项目负责人在计划中大量使用。核心设备齐全， 提供转基因/敲除以及吸烟服务，并利用我们的专用空间 最先进的动物设施来实现这些功能。此外，鼠标核心将支持 围绕BUXCO系统使用的基础设施，以实现侵入性抵抗和合规性 测量.这些研究将由Michael J. Holtzman博士监督，并由Suzanne女士完成 Swanson说，他是一位研究技术人员，在使用这个系统方面有相当的专业知识。

The major objective of the Mouse Core is to provide a centralized service oriented facility to insure the capacity of each participating project lab to produce and maintain transgenic and gene-disrupted mice. The facility was developed in 1995 within the Pulmonary Division. Initially, the facility was equipped using funds from the Barnes-Jewish Hospital Foundation, and has historically primarily served labs involved in pulmonary research. More recently, the facility has been supported largely by two NIH grants, including a SCOR grant on asthma (Mechanisms of Airway Inflammation and Remodeling, P50 HL56419, Michael J. Holtzman, PI), and a Program Project Grant (Mechanisms in the Remodeling of Lung Structure, P01 HL29594, Robert M. Senior, PI). As most of the projects in this application propose genetic experiments in mice, we propose that this grant also support a proportional part of the Mouse Core. Many of the Project Leaders, Co-Investigators, and Core Leaders on this application (Holtzman, Mecham, Pierce, Senior, Walter, Urban, Atkinson, Brody, Shipley) have been utilizing it for several years for work related to these two grants as well as other projects. Since 1995, the facility has generated more than 100 lines of transgenic and gene-targeted mice, with multiple founders or chimeras of each line in most cases. This has resulted in an economy of scales by minimizing overhead and avoiding duplication of a highly technical skill requiring specialized equipment and animal facilities. The Mouse Core is a major avenue for collaborations. The ability to perform gain of function and loss of function experiments in vivo has and will enhance our understanding of the molecular mechanisms governing remodeling of the lung in COPD. Additionally, the core has served to help develop a murine model of cigarette smoke-induced pulmonary emphysema. In this application, the core will also support the infrastructure of this model, as it will be heavily utilized by most Project Leaders in the program. The core is fully equipped to provide both transgenic/knockout as well as cigarette smoking services, and utilizes dedicated space within our state of the art animal facility to fulfill these functions. In addition, the Mouse Core will support the infrastructure surrounding use of the BUXCO system for making invasive resistance and compliance measurements. These studies will be overseen by Dr. Michael J. Holtzman and will be done by Ms. Suzanne Swanson, a research techinican who has considerable expertise using this system.