Core C

核心C

• 批准号: 6823521
• 负责人: J MICHAEL SHIPLEY
• 金额: $ 11.37万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6823521
• 关键词: biomedical facility; biotechnology; disease /disorder model; gene targeting; genetically modified animals; inflammation; laboratory mouse; lung; lung development; lung injury; microinjections; regeneration; smoke inhalation; tobacco; transfection /expression vector

The purpose of the Mouse Core will be to provide a centralized service oriented facility to insure the capacity of each participating project lab to produce and maintain transgenic and gene-disrupted mice. The Mouse Core will also serve the projects by supporting the infrastructure of a lung injury model that will be extensively utilized by most of the projects, namely exposure to cigarette smoke. As most of the projects propose to make multiple transgenic or gene-targeted lines of mice and to test these lines in the smoking model, the Mouse Core will be an important part of this Program Project Grant. The two primary components of the Mouse Core, the transgenic/knockout production unit and the smoking facility, are both supported by dedicated space within our animal facility and are fully equipped. While not previously part of this Program Project Grant, this core was developed in our Pulmonary Division in 1995 by Dr. Steven D. Shapiro, a project leader on this application, and has been extensively utilized by Project Investigators and Core Directors on this application (Senior, Shapiro, Holtzman, Parks, Shipley). It is staffed by personnel with a collective extensive track record of expertise and productivity in this area. The core has generated over 75 separate transgenic and knockout lines, with multiple founders/chimeras of each. Many of the mice which have been and will be made by this core will facilitate collaborative interactions amongst Project Investigators. As this Program would support 50% of the transgenic/knockout production unit, which will be utilized by Program Investigators as well as other investigators at Washington University, this will allow us to realize an economy of scales and lower production costs per mouse generated.

小鼠核心的目的是提供一个集中服务导向的设施，以确保每个参与项目实验室生产和维持转基因和基因破坏小鼠的能力。 Mouse Core 还将通过支持肺损伤模型的基础设施来服务这些项目，该模型将被大多数项目广泛使用，即暴露于香烟烟雾。由于大多数项目都提议制造多个转基因或基因靶向小鼠品系，并在吸烟中测试这些品系 模型中，Mouse Core 将成为该计划项目资助的重要组成部分。 Mouse Core 的两个主要组成部分，即转基因/基因敲除生产单元和吸烟设施，均由我们动物设施内的专用空间提供支持，并且设备齐全。虽然之前不是该计划项目资助的一部分，但该核心是由该应用程序的项目负责人 Steven D. Shapiro 博士于 1995 年在我们的肺科开发的，并已被该应用程序的项目研究者和核心主管（Senior、Shapiro、Holtzman、Parks、Shipley）广泛使用。它的员工在该领域拥有丰富的专业知识和生产力记录。该核心已产生超过 75 个独立的转基因和敲除品系，每个品系都有多个创始人/嵌合体。许多已经和将要由该核心制造的小鼠将促进项目研究人员之间的协作互动。由于该计划将支持 50% 的转基因/基因敲除生产单位，这些单位将由计划研究人员以及华盛顿大学的其他研究人员使用，这将使我们能够实现规模经济并降低每只小鼠的生产成本。