Core C

小鼠核心的目的是提供一个集中的面向服务的设施，以确保每个参与项目的实验室生产和维护转基因和基因破坏小鼠的能力。Mouse Core还将通过支持肺损伤模型的基础设施来服务于这些项目，该模型将被大多数项目广泛使用，即暴露于香烟烟雾。由于大多数项目都提出了制造多个转基因或基因靶向小鼠品系，并在吸烟环境中测试这些品系， 模型，鼠标核心将是这个计划项目资助的重要组成部分。小鼠核心的两个主要组成部分，转基因/敲除生产单元和吸烟设施，都由我们动物设施内的专用空间提供支持，并且设备齐全。虽然以前不是本计划项目赠款的一部分，但该核心是由Steven D. Shapiro，该应用程序的项目负责人，并已被项目研究人员和核心总监广泛用于该应用程序（高级，Shapiro，Holtzman，Parks，Shipley）。它的工作人员在这一领域具有广泛的专门知识和生产力。核心已经产生了超过75个单独的转基因和敲除品系，每个品系具有多个创始者/嵌合体。许多已经和将要由该核心制造的小鼠将促进项目研究者之间的协作互动。由于该计划将支持50%的转基因/敲除生产单位，这将由计划研究者以及华盛顿大学的其他研究者使用，这将使我们能够实现规模经济和降低每只小鼠的生产成本。

The purpose of the Mouse Core will be to provide a centralized service oriented facility to insure the capacity of each participating project lab to produce and maintain transgenic and gene-disrupted mice. The Mouse Core will also serve the projects by supporting the infrastructure of a lung injury model that will be extensively utilized by most of the projects, namely exposure to cigarette smoke. As most of the projects propose to make multiple transgenic or gene-targeted lines of mice and to test these lines in the smoking model, the Mouse Core will be an important part of this Program Project Grant. The two primary components of the Mouse Core, the transgenic/knockout production unit and the smoking facility, are both supported by dedicated space within our animal facility and are fully equipped. While not previously part of this Program Project Grant, this core was developed in our Pulmonary Division in 1995 by Dr. Steven D. Shapiro, a project leader on this application, and has been extensively utilized by Project Investigators and Core Directors on this application (Senior, Shapiro, Holtzman, Parks, Shipley). It is staffed by personnel with a collective extensive track record of expertise and productivity in this area. The core has generated over 75 separate transgenic and knockout lines, with multiple founders/chimeras of each. Many of the mice which have been and will be made by this core will facilitate collaborative interactions amongst Project Investigators. As this Program would support 50% of the transgenic/knockout production unit, which will be utilized by Program Investigators as well as other investigators at Washington University, this will allow us to realize an economy of scales and lower production costs per mouse generated.