Biochemical, molecular and immunological characterization of Mycoplasma pneumoni
Biochemical, molecular and immunological characterization of Mycoplasma pneumoni
批准号:
8328001
负责人:
JOEL Barry BASEMAN
金额:
$42.24万
依托单位国家:
美国
项目类别:
财政年份:
2011
资助国家:
美国
项目状态:
已结题
起止时间:
2011-08-01 至 2016-07-31
关键词:
ADP ribosylationAcuteAdultAdult Respiratory Distress SyndromeAllergic inflammationAlveolarAmino AcidsAnimal ModelAntibodiesAsthmaBindingBiochemicalBiologicalBiological AssayCellsChemicalsChestChildChronicClinicalCollectionCommunity Acquired Respiratory Distress Syndrome ToxinDendritic CellsDevelopmentDiagnosisDiagnosticDiphtheria ToxinDiseaseEpithelialEpitopesEssential Amino AcidsFibroblastsFunctional disorderFutureGoalsHumanImmuneIncidenceIndividualInfectionInflammationInflammatoryInjuryKnowledgeLeadLengthLinkLungLymphocyteMammalian CellMediatingMediator of activation proteinMolecularMonitorMonoclonal AntibodiesMusMycoplasmaMycoplasma pneumoniaePassive ImmunizationPathologyPathway interactionsPatientsPersonal SatisfactionPertussis ToxinPhenotypePneumoniaPopulationPredispositionProgress ReportsPropertyProteinsPulmonary Surfactant-Associated Protein APyroglyphidaeReagentRelative (related person)ResearchResistanceRoleSamplingSiteSpecimenStructureStructure of respiratory epitheliumTestingTherapeutic AgentsTissuesToxinTranscriptional RegulationTransferaseTranslational RegulationTraumaVentilatorVirulenceclinically relevantdesignimprovedinnovationmacrophagemicrobialneutralizing antibodynovelpathogenpolyclonal antibodypreventreceptorsmall moleculetherapeutic vaccinevaccine candidate
中文摘要
肺炎支原体是一种常见的非典型细菌性病原体,与急性肺炎密切相关
英文摘要
Mycoplasma pneumoniae is a common atypical bacterial pathogen strongly associated with acute and
chronic airway diseases, including new-onset asthma and exacerbations, as well as persistent infections in
children and adults. Knowledge on how M. pneumoniae causes infection with accompanying inflammatory
pathways, pathologies and sequelae has been deficient. However, our identification of the Community
Acquired Respiratory Distress Syndrome ToXin (CARDS TX), a bona fide ADP ribosylating and vacuolating
toxin, has provided a direct causal link between M. pneumoniae and ainway inflammation and injury. This
connection between M. pneumoniae, CARDS TX and lung hyper-responsiveness and inflammation is
reinforced by SA-AADCRC research efforts demonstrating M. pneumoniae and CARDS TX direct clinical
relevance and further supported by animal modeling and innovative diagnostic, pathological, biological,
chemical and immunological assessments. Considering these findings and the structural and functional
similarities and potential pathogenic significance of CARDS TX relative to pertussis and diphtheria toxins, we
hypothesize that CARDS TX is a critical pathogenic contributor to M. pneumoniae-mediated asthma and
related ainway diseases. To test this hypothesis, we will: 1. Characterize CARDS TX-mediated ADP-ribosyl
transferase (ART) activity by detecting the CARDS TX minimal domain essential for ART function and by
identifying the mammalian target proteins that are ADP-ribosylated by CARDS TX. 2. Characterize the
CARDS TX binding region by identifying the minimal region(s) and essential amino acids that mediate
binding to surfactant protein-A and mammalian cell receptor(s). 3. Generate monoclonal and polyclonal
antibodies reactive against CARDS TX and determine epitopes of CARDS TX capable of inducing
blocking/neutralizing antibodies. In the latter case, the availability of antibody reagents that neutralize and
block CARDS TX activities, along with the collaborative studies outlined throughout this SA-AADCRC
application should assist in the innovative design of therapeutic agents and improved diagnostics.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Role of host cell invasion in Mycoplasma genitalium persistent infection
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批准号:9197256
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项目类别:
-
资助金额:$19.06万
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财政年份:2015
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负责人:JOEL Barry BASEMAN
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依托单位:
Administrative Core
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批准号:8328002
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项目类别:
-
资助金额:$19.45万
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财政年份:2011
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负责人:JOEL Barry BASEMAN
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依托单位:
Infrastructure and Opportunity Fund Management
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批准号:8328003
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项目类别:
-
资助金额:$83.49万
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财政年份:2011
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负责人:JOEL Barry BASEMAN
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依托单位:
San Antonio STI TM CRC
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批准号:7921828
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项目类别:
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资助金额:$54.72万
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财政年份:2009
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负责人:JOEL Barry BASEMAN
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依托单位:
Role of unique ADP-ribosylating vacuolating Mycoplasma pneumoniae toxin in asthma
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批准号:7914874
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项目类别:
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资助金额:$40.79万
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财政年份:2009
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负责人:JOEL Barry BASEMAN
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依托单位:
Administrative Core
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批准号:7686483
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项目类别:
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资助金额:$11.1万
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财政年份:2008
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负责人:JOEL Barry BASEMAN
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依托单位:
Biochemical, Molecular &Immunological Characterization of the Mycoplasma pneumon
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批准号:7686482
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项目类别:
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资助金额:$26.31万
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财政年份:2008
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负责人:JOEL Barry BASEMAN
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依托单位:
Discretionary Projects
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批准号:7686466
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项目类别:
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资助金额:$48.01万
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财政年份:2008
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负责人:JOEL Barry BASEMAN
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依托单位:
Role of unique ADP-ribosylating vacuolating Mycoplasma pneumoniae toxin in asthma
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批准号:7274288
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项目类别:
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资助金额:$155.53万
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财政年份:2006
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负责人:JOEL Barry BASEMAN
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依托单位:
Role of unique ADP-ribosylating vacuolating Mycoplasma pneumoniae toxin i
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批准号:8300811
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项目类别:
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资助金额:$203.36万
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财政年份:2006
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负责人:JOEL Barry BASEMAN
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依托单位:
Role of unique ADP-ribosylating vacuolating Mycoplasma pneumoniae toxin in asthma
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批准号:7682873
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项目类别:
-
资助金额:$149.49万
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财政年份:2006
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负责人:JOEL Barry BASEMAN
-
依托单位:
Role of unique ADP-ribosylating vacuolating Mycoplasma pneumoniae toxin i
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批准号:8705990
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项目类别:
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资助金额:$255.36万
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财政年份:2006
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负责人:JOEL Barry BASEMAN
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依托单位:
Role of unique ADP-ribosylating vacuolating Mycoplasma pneumoniae toxin i
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批准号:8897848
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项目类别:
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资助金额:$205.36万
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财政年份:2006
-
负责人:JOEL Barry BASEMAN
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依托单位:
Role of unique ADP-ribosylating vacuolating Mycoplasma pneumoniae toxin i
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批准号:8164349
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项目类别:
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资助金额:$311.22万
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财政年份:2006
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负责人:JOEL Barry BASEMAN
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依托单位:
Role of unique ADP-ribosylating vacuolating Mycoplasma pneumoniae toxin i
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批准号:8513872
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项目类别:
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资助金额:$193.04万
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财政年份:2006
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负责人:JOEL Barry BASEMAN
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依托单位:
Biochemical, Molecular and Immunological Characterization of the Mycoplasma pneum
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批准号:7150764
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项目类别:
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资助金额:$22.08万
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财政年份:2006
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负责人:JOEL Barry BASEMAN
-
依托单位:
Role of unique ADP-ribosylating vacuolating Mycoplasma pneumoniae toxin in asthma
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批准号:7134895
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项目类别:
-
资助金额:$150.79万
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财政年份:2006
-
负责人:JOEL Barry BASEMAN
-
依托单位:
Administrative Core
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批准号:7150765
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项目类别:
-
资助金额:$9.42万
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财政年份:2006
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负责人:JOEL Barry BASEMAN
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依托单位:
Role of unique ADP-ribosylating vacuolating Mycoplasma pneumoniae toxin in asthma
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批准号:7904191
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项目类别:
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资助金额:$150.08万
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财政年份:2006
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负责人:JOEL Barry BASEMAN
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依托单位:
MYCOPLASMA PNEUMONIAE: AIRWAY INTERPLAY
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批准号:7349846
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项目类别:
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资助金额:$1.16万
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财政年份:2006
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负责人:JOEL Barry BASEMAN
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依托单位:
海外基金