NINDS INSTITUTIONAL CENTER CORE GRANTS - Neuroproteomics P30

NINDS 机构中心核心资助 - 神经蛋白质组学 P30

• 批准号: 8145845
• 负责人: RICHARD S MORRISON
• 金额: $ 25.95万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2013-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8145845
• 关键词: NINDS; INSTITUTIONAL; CENTER; CORE; GRANTS

The purpose of this proposal is to establish a University of Washington Research Core facility that provides essential proteomics and bioinformatics infrastructure support for a large contingent of scientists interested in mapping protein-protein interactions in the nervous system. Many of these investigators conduct research involving disease genes and a defined analysis of their binding partners will help investigators to understand how alterations in these genes modulate neuronal and glial structure and function during the course of nervous system disease and injury. This research is clearly essential to the mission of the NINDS. Three research cores and one administrative core will provide support for the research programs and will stimulate interactions and collaborations among investigators. Core A, the molecular virology core, will provide a repository for all of the expression plasmids needed to tag proteins and map protein-protein interactions and validate binding interactions using a variety of biochemical methods. This core will also generate and titer lentivirus for each expression construct providing a valuable reagent for expressing tagged proteins in cells that are traditionally difficult to transfect. Core B, the mass spectrometry core, will provide mass spectrometry-based identification of protein complexes purified using the pull down techniques associated with the various tagged proteins generated in Core A. Core C, the bioinformatics core, will provide comprehensive analysis of protein sequence data, molecular interactions, pathways, biological structures, genetic maps, homology information, and functional annotations of proteins identified in the various pull down assays. The research cores will be supported by an administrative core that will oversee the operation of each research core, organize user meetings, steering committee meetings, organize an annual research symposium, provide clerical, fiscal and personnel support, and prepare reports to the funding agency.

该提案的目的是建立一个华盛顿大学研究核心设施，为有兴趣绘制神经系统中蛋白质-蛋白质相互作用的大量科学家提供必要的蛋白质组学和生物信息学基础设施支持。这些研究人员中的许多人进行涉及疾病基因的研究，并且对其结合伴侣进行定义分析将有助于研究人员了解这些基因的改变如何在神经系统疾病和损伤过程中调节神经元和神经胶质的结构和功能。这项研究显然对NINDS的使命至关重要。三个研究核心和一个行政核心将为研究项目提供支持，并将促进研究人员之间的互动和合作。核心A，分子病毒学核心，将提供一个储存库的所有表达质粒需要标记蛋白质和地图蛋白质-蛋白质相互作用和验证结合相互作用使用各种生化方法。该核心还将为每个表达构建体产生和滴定慢病毒，为在传统上难以转染的细胞中表达标记蛋白提供有价值的试剂。核心B（质谱核心）将提供基于质谱的蛋白质复合物鉴定，该蛋白质复合物使用与核心A中生成的各种标记蛋白质相关的下拉技术纯化。核心C，生物信息学核心，将提供蛋白质序列数据，分子相互作用，途径，生物结构，遗传图谱，同源性信息和各种下拉分析中鉴定的蛋白质的功能注释的综合分析。研究中心将由一个行政中心提供支助，该行政中心将监督每个研究中心的运作，组织用户会议、指导委员会会议，组织年度研究专题讨论会，提供文书、财务和人事支助，并编写提交供资机构的报告。