Core B

我们一直在寻求采用新的方法来增强 多肽的分离与鉴定。我们很幸运有大卫·古德利特博士作为核心董事，因为他 积极参与新的质谱学技术的开发(请参阅他的生物草图和 字母)。我们还聘请了一位国际公认的质谱学和生物信息学专家， 蒂莫西·维恩斯特拉博士担任外部顾问。这两个人将确保核心C提供状态 为我们的最终用户提供最先进的技术。未来的一个方向将是纳入稳定的同位素标记 方法，例如同位素编码亲和标签(ICAT)或iTRAQ方法，以及所谓的无标记方法，如 最近在古德利特博士的实验室中进行了描述(Foss等人，发表在Press，自然遗传学)。这将使我们能够 提供有关蛋白质在复合体和辨别真实性中的相对表达水平的信息 复合体的成员来自非特定的、污染的蛋白质58。此外，取决于我们的 资源，有可能提供我们先前为小鼠描述的全球蛋白质组分析 皮质神经元57和小鼠星形胶质细胞(McBee等人，在出版社，蛋白质组学；见Morrison biosketch)。

We are constantly looking to employ new methods to enhance the separation and identification of peptides. We are fortunate to have Dr. David Goodlett as a core director as he is actively involved in the development of new mass spectrometry techniques (please see his biosketch and letter). We have also recruited an internationally recognized expert on mass spectrometry and bioinformatics, Dr. Timothy Veenstra, as an external consultant. These two individuals will insure that Core C provides state of the art advances to our end users. One future direction will involve incorporating stable isotope labeling methods, e.g. isotope coded affinity tag (ICAT) or iTRAQ methods, as well as so-called label-free methods as recently described in Dr. Goodlett's laboratory (Foss et al., In Press, Nature Genetics). This will allow us to provide information on the relative levels of expression of proteins in a complex and discern bona fide members of the complexes from non-specific, contaminating proteins58. In addition, depending on our resources, it may be possible to provide global proteome analysis as we previously described for mouse cortical neurons57 and mouse astrocytes (McBee et al, In Press, Proteomics; see Morrison Biosketch).