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Visualization of Biological Complexity Resource

生物复杂性资源可视化

基本信息

批准号：
8033294
负责人：
CARMEN A. MANNELLA
金额：
$ 108.13万
依托单位：
WADSWORTH CENTER
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-01-01 至 2011-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8033294
关键词：
Achievement Agreement Algorithms Area Beds Biological Cell physiology Cells Classification Collaborations Communities Complex Computer software Data Collection Data Set Deposition Development Devices Documentation Dose Electron Microscope Electron Microscopy Freezing Heterogeneity Housing Image Imagery Institutes Ions Lead Life Methodology Methods Modality Molecular Profiling Morphologic artifacts Nanotechnology Organelles Phase Population Preparation Procedures Process Reaction Request for Applications Research Resolution Resources Ribosomes Ryanodine Receptor Calcium Release Channel Ryanodine Receptors Services Software Design Specimen Spiders Techniques Technology Test Result Testing Time Tissues Ultramicrotomy base biological systems design flash photolysis imaging modality improved macromolecular assembly method development millisecond nanofabrication new technology novel operation particle pressure prototype reconstruction research and development software development tomography

项目摘要

The Wadsworth Center's Resource for the Visualization of Biological Complexity (RVBC) employs threedimensional (3D) electron microscopy at different levels of resolution to study cell processes in the native context and at close-to-native conditions, and to capture the hierarchical organization of living matter, (i) Low-dose cryo-tomography of frozen-hydrated cell and tissue sections will be further developed by utilizing novel techniques such as ion beam milling, through a collaboration with SUNY Albany, to reduce cutting artifacts. Efforts will be made to optimize data collection, alignment, and reconstruction to achieve a range of resolution where molecular signatures can be recognized, (ii) Macromolecular interactions will be studied as dynamic processes by 3D cryo-imaging of complexes in single-particle form and by development of timeresolved imaging methods, using the rich test bed provided by in-house projects such as the ryanodine receptor and the ribosome. In addition to spray-freezing and use of UV-activated caged compounds, we will explore the use of novel nanofabrication methods in designing EM grids, in a consortium agreement with the Rensselaer Polytechnic Institute, that can act as mixing platforms, (iii) Heterogeneity is recognized as the single biggest remaining obstacle to the achievement of higher resolution in single-particle reconstruction of macromolecular assemblies. General methods of supervised and unsupervised classification will be developed in collaboration with lead experts, using both phantom and experimental data sets that represent typical problems encountered in the field. In addition to advancing these three areas of technological and research development, which are driven by research at the Wadsworth Center and outside collaborations, we continue to offer the unique facilities of the Resource to the Biological Community in service projects.

沃兹沃斯中心的生物复杂性可视化资源（RVBC）采用三维