Characterization of the Desmosome Protein Perp

桥粒蛋白 Perp 的表征

• 批准号: 8098865
• 负责人: LAURA D ATTARDI
• 金额: $ 32.89万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8098865
• 关键词: Address; Adherens Junction; Adhesions; Adult; Affect; Affinity Chromatography; Aging; Antibodies; Biological Assay; Cell membrane; Cell-Cell Adhesion; Co-Immunoprecipitations; Cytoplasmic Tail; Defect; Desmosomes; Development; Disease; Ectoderm; Epidermis; Epithelial; Functional disorder; Future; Genetic; Growth Factor; Hair; Hair follicle structure; Homeostasis; Knock-out; Knockout Mice; Lead; Maintenance; Malignant Neoplasms; Membrane; Membrane Proteins; Molecular; Molecular Mechanisms of Action; Mouse Strains; Mus; Mutation; Nail plate; Nature; Neonatal; Pathway interactions; Pemphigus Vulgaris; Phenotype; Physiological; Play; Post-Translational Protein Processing; Process; Program Development; Proteins; Regulation; Research Personnel; Role; Series; Skin; Stimulus; Structure; Sweat Glands; Tissues; Tooth structure; Transmembrane Domain; Wound Healing; appendage; base; defined contribution; extracellular; human disease; insight; loss of function; mutant; novel; programs; research study; trafficking; tumor progression

DESCRIPTION (provided by applicant): Human diseases associated with defects in cell-cell adhesion junctions known as desmosomes have suggested the importance of desmosomal components for the function and maintenance of the skin and ectodermal derivatives, including hair, teeth, nails, and sweat glands. Using knockout mice, we recently identified the Perp tetraspan membrane protein as a component of the p63 stratified epithelial development program, where it plays an essential role in desmosome function and epithelial adhesion in the skin. As a critical desmosomal constituent, Perp inactivation might lead to dysfunction of ectodermal derivatives. To address this hypothesis, we propose to use conditional mice we generated to ablate Perp specifically in the ectoderm and its derivatives and to determine the phenotypes arising in aging adult mice. Through this analysis, we will define the role of Perp in hair follicles, nails, teeth and sweat glands. As the role of desmosomes in ectoderm derivatives is not well understood, our studies will provide new insight into their role in these contexts. Moreover, revealing the consequences of Perp-deficiency in these tissue compartments provides a basis for identifying human diseases associated with Perp-deficiency in the future. To gain insight into the mechanism of Perp action at the desmosome, we will define important functional motifs within Perp by generating a panel of Perp mutants with alterations in specific domains and we will identify Perp-interacting desmosomal proteins. Finally, we propose to examine the role of Perp in the dynamic assembly and disassembly of desmosomes. To determine how Perp promotes desmosomal adhesion, we will establish whether Perp enhances trafficking, clustering or stability of desmosomal proteins at the plasma membrane. To define Perp's role in desmosome dissolution induced by a variety of physiological and pathological stimuli, including growth factors, wounding, and Pemphigus Vulgaris antibodies, we will examine Perp expression, localization and post-translational modification in these settings. These experiments will provide insight into a larger role that Perp may play in desmosome remodeling during such processes as development, wound healing, and cancer. Together, these approaches will provide an understanding of how Perp, desmosomes, and p63 contribute to both epithelial integrity and ectodermal appendage function, and how their dysfunction leads to disease.

描述（由申请人提供）：与细胞-细胞粘附连接（称为桥粒）缺陷相关的人类疾病表明了桥粒组分对皮肤和外胚层衍生物（包括毛发、牙齿、指甲和汗腺）的功能和维持的重要性。使用基因敲除小鼠，我们最近确定了Perp tetraspan膜蛋白作为p63分层上皮发育程序的一个组成部分，它在皮肤中的桥粒功能和上皮粘附中起着至关重要的作用。Perp作为桥粒的重要成分，其失活可能导致外胚层衍生物功能障碍。为了解决这一假设，我们建议使用条件小鼠，我们产生的消融Perp特异性外胚层及其衍生物，并确定老化的成年小鼠中出现的表型。通过这种分析，我们将定义Perp在毛囊，指甲，牙齿和汗腺中的作用。由于桥粒在外胚层衍生物中的作用尚不清楚，我们的研究将为它们在这些背景下的作用提供新的见解。此外，揭示这些组织区室中Perp缺乏的后果为将来识别与Perp缺乏相关的人类疾病提供了基础。为了深入了解Perp作用于桥粒的机制，我们将通过产生一组在特定结构域中具有改变的Perp突变体来定义Perp内的重要功能基序，并且我们将鉴定Perp相互作用的桥粒蛋白。最后，我们建议研究Perp在桥粒动态组装和拆卸中的作用。为了确定Perp如何促进桥粒粘附，我们将确定Perp是否增强桥粒蛋白在质膜上的运输、聚集或稳定性。为了定义Perp在由各种生理和病理刺激诱导的桥粒溶解中的作用，包括生长因子，创伤和寻常型天疱疮抗体，我们将研究Perp在这些环境中的表达，定位和翻译后修饰。这些实验将提供一个更大的作用，Perp可能发挥桥粒重塑过程中的发展，伤口愈合和癌症的洞察。总之，这些方法将提供一个理解Perp，桥粒，和p63有助于上皮完整性和外胚层附件功能，以及它们的功能障碍如何导致疾病。