TRIAD CMI Scientific Core

整个计划的主题是开发广谱疫苗技术过程 使用免疫信息学工具进行表位预测。许多流程和程序将是 该计划中的项目使用人类白细胞、人类白细胞抗原转基因小鼠和检测 细胞介导的免疫反应的特征，以验证计算机预测和分析免疫 候选疫苗引起的反应。这种细胞介导的免疫科学核心旨在提供 支持这些核查过程和分析所需的设备、人员配置和能力。全 该计划内的研究和技术开发项目规定了部分或全部资源 由该核心提供的数据将被每个项目使用。它提供的核心功能和基础设施 核心将包括以下内容： 1)流式细胞仪和细胞因子珠分析 2)ELISpot分析 3)人白细胞超低温保存和短期保存 4)通过支持人类白细胞抗原转基因小鼠，确保程序获得所需的人类白细胞抗原转基因小鼠 塔肯农场的繁殖群

The theme of the overall program focuses on development of a broad spectrum vaccine technology process employing immunoinformatics tools for epitope prediction. Many of the processes and procedures to be employed by projects within the program employ human leucocytes, HLA transgenic mice, and assays for characterization of cell mediated immune responses to verify the in silico predictions and to analyze immune responses engendered by vaccine candidates. This cell mediated immunity scientific core is designed to provide the equipment, staffing, and capabilities required to support these verification processes and analyses. All research and technology development projects within the program specify that some or all of the resources provided by this core will be employed by each project. The core capabilities and infrastructure provided by this core will include the following: 1) Flow cytometric and cytokine bead analysis 2) ELISpot assay analysis 3) Human leucocyte cryopreservation and short term storage 4) Insuring program access to required HLA transgenic mice through support of HLA transgenic murine breeding colony at Taconic Farms