TRIAD CMI Scientific Core

TRIAD CMI 科学核心

• 批准号: 8501262
• 负责人: Gregory Paquette
• 金额: $ 31.29万
• 依托单位: UNIVERSITY OF RHODE ISLAND
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8501262
• 关键词: Address; Antigens; Binding; Biological Assay; Breeding; Burkholderia; Cellular Immunity; Chronic; Computer Simulation; Cryopreservation; Development; Disease; Engineering; Epitopes; Equipment; Evaluation; Farming environment; Helicobacter pylori; Hepatitis C virus; Human; Immune response; Immunology; In Vitro; Leukocytes; Measures; Methods; Mus; Procedures; Process; Research; Research Infrastructure; Resources; Specific qualifier value; Technology; Testing; Ticks; Transgenic Mice; Transgenic Organisms; Tularemia; Vaccines; Work; cell mediated immune response; cytokine; design; in vivo; instrumentation; pathogen; programs; research and development; technology development; vaccine candidate; vaccine development

The theme of the overall program focuses on development of a broad spectrum vaccine technology process employing immunoinformatics tools for epitope prediction. Many of the processes and procedures to be employed by projects within the program employ human leucocytes, HLA transgenic mice, and assays for characterization of cell mediated immune responses to verify the in silico predictions and to analyze immune responses engendered by vaccine candidates. This cell mediated immunity scientific core is designed to provide the equipment, staffing, and capabilities required to support these verification processes and analyses. All research and technology development projects within the program specify that some or all of the resources provided by this core will be employed by each project. The core capabilities and infrastructure provided by this core will include the following: 1) Flow cytometric and cytokine bead analysis 2) ELISpot assay analysis 3) Human leucocyte cryopreservation and short term storage 4) Insuring program access to required HLA transgenic mice through support of HLA transgenic murine breeding colony at Taconic Farms

整个计划的主题集中在广谱疫苗技术过程的发展 使用免疫信息学工具进行表位预测。许多流程和程序将 项目内的项目采用人类白细胞、HLA转基因小鼠， 表征细胞介导的免疫应答，以验证计算机模拟预测并分析免疫应答。 候选疫苗产生的反应。这种细胞介导的免疫科学核心旨在提供 支持这些核查过程和分析所需的设备、人员配备和能力。所有 该计划中的研究和技术开发项目规定， 每个项目都将使用该核心提供的资源。它所提供的核心能力和基础设施 核心将包括以下内容： 1)流式细胞术和细胞因子珠分析 2)ELISpot分析 3)人白细胞的低温保存和短期保存 4)通过HLA转基因小鼠的支持确保项目获得所需的HLA转基因小鼠 Taconic农场的繁殖群