CIRCUIT ANALYSIS OF PFC AND MTL INTERACTIONS IN MONKEYS

项目总结（见说明）： 这个项目解决了如何使用非人类灵长类动物模型形成记忆的功能电路的作用。尽管有大量的证据表明，前额叶皮层（PFC）和内侧颞叶（MTL）对正常的记忆都是至关重要的，但对于理解这两个系统如何相互作用的实验努力却非常少。 主要侧重于PFC或MTL。因此，我们对它们的大部分了解都是基于不同动物之间的比较，这些动物具有不同的训练历史，通常是不同的任务，和/或不同的经验水平。这混淆了它们的比较，并排除了对它们活动的精确时间的检查，从而深入了解网络特性和信号流。我们的目标是 对PFC和MTL的综合理解。我们将通过在PFC和MTL的多个子区域中的多个电极同时记录来实现这一点，同时猴子形成并回忆新的上下文引导的联想记忆，同时他们根据这些记忆进行推理。通过比较记忆形成和回忆的相对神经延迟，我们将确定记忆在哪里形成，如何回忆，以及与记忆相关的信号如何在PFC和MTL之间流动。

PROJECT SUMMARY (See instructions): This project addresses the role of functional circuitry of how memories are formed using a non-human primate model. Despite the great deal of evidence that both the prefrontal cortex (PFC) and medial temporal lobe (MTL) are critical for normal memory, there has been remarkably little experimental effort toward understanding how these two systems interact This is, in part, because neurophysiological studies have largely focused on either the PFC or MTL individually. As a result, much of what we know about them is based on comparisons between different animals with different training histories, often on different tasks, and/or different levels of experience. This confounds their comparison and precludes examination of the precise timing of their activity that gives insight into network properties and signal flow. Our goal is a more integrated understanding of the PFC and MTL. We will accomplish this by recording simultaneously from multiple electrodes in multiple subregions of the PFC and MTL while monkeys form and recall new context guided associative memories and while they make inferences based on those memories. By comparing the relative neural latencies for memories to be formed and recalled, we will determine where memories are formed, how they are recalled, and how memory-related signals flow between the PFC and MTL.