Multiuser facility for flow cytometry and sorting

描述(申请人提供)：流式细胞术是一种自动化方法，用于根据大小和荧光强度对活细胞或固定细胞进行分析和分选，使用多个荧光探针，根据细胞的固有属性(例如DNA)或独特蛋白质抗原的表达来标记细胞。荧光激活细胞分选器可以根据活细胞的抗原或蛋白质表达谱分离单个活细胞(分选)。独特和稀有的细胞类型，如干细胞或处于特定发育阶段的细胞，可以从混合细胞中无菌分离出来，并进行鉴定或培养。流式细胞术已经成为健康科学的许多领域的关键--包括癌症、免疫学和再生医学。这项建议的目的是为NIH资助的一大批研究人员提供最先进的流式细胞术和细胞分类。此外，该设备将使更多偶尔进行流式细胞仪实验或细胞分离的用户受益。我们将更换已有15年历史的BD Vantage细胞分选仪，该仪器的维护不再受制造商的支持。这种新型iCyt流式细胞仪的速度和性能比它正在取代的Vantage分选机有了很大的提高，并且它还具有在生物密封罩中进行无菌分选的额外能力，这一功能极大地增加了它对密歇根州立大学和研究社区的实用性。描述了12个单独的研究项目，范围从对艾滋病毒、流感、登革热和腺病毒感染的细胞进行分类；到从人类肠道微生物群中分离细菌。由于建议的仪器完全包含在层流环境中，它将能够进行生物危害隔离和执行严格的无菌。

DESCRIPTION (provided by applicant): Flow cytometry is an automated method for the analysis and sorting of live or fixed cells based on size and fluorescent intensity using multiple fluorescent probes that label cells based on intrinsic properties (e.g. DNA) or expression of unique protein antigens. Fluorescent activated cell sorters allow for the isolation of individual live cells (sorting) based on their antigen or protein expression profiles. Unique and rare cell types, such as stem cell or cells in specific developmental stages, can be isolated sterilely from mixtures of cells and characterized or cultured. Flow cytometry has become critical to many areas of health sciences - including cancer, immunology and regenerative medicine. The objective of this proposal is to provide state-of-the-art flow cytometry and cell sorting to a large group of NIH funded investigators. In addition, the equipment will benefit a larger group of users who perform occasional flow cytometry experiments or cell isolations. We will replace a fifteen year-old BD Vantage cell sorter instrument whose maintenance is no longer supported by the manufacturer. This speed and performance of this new iCyt flow cytometer is much improved over the Vantage sorter it is replacing, and it has additional capability to carry out sterile sorting in a biocontainment hood, a feature that greatly increases its utility to the MSU the research community. Twelve individual research projects are described that range from sorting HIV, influenza, dengue and adenovirus infected cells; to the isolation of bacteria from the human gut microbiome. Since the proposed instrument is completely contained in a laminar flow environment, it will be capable of biohazard isolation and enforcement of strict sterility.