IVIS Spectrum Imaging System

描述（由申请人提供）：2009年6月，海伦迪勒家庭综合癌症中心（HDFCCC）开设了新的国家的最先进的海伦迪勒家庭癌症研究大楼（HDFCRB）位于发展中的使命湾校园。该大楼目前容纳了30多名PI，从事基础，临床前和转化癌症研究。通过完成实验室和办公空间的额外楼层，有可能进一步扩大50%。该建筑有一个现代化的动物园，在屏障设施内饲养小动物。该设施有可能容纳13000个动物笼子，每个笼子最多可容纳5只成年小鼠。到2014年，当新的289张床位的UCSF儿童妇女和癌症医院在使命湾开业时，这一额外的能力肯定是需要的。锡安临床研究团队。HDFCRB的开放将大量癌症研究科学家聚集在一个屋檐下。然而，开放代表了癌症研究在加州大学旧金山分校的扩展，而不是完全巩固。HDFCCC的规模，有300多个PI分布在旧金山市的三个主要地点，在每个地点提供必要的基础设施，特别是高端仪器，以支持基础，临床前和转化研究方面提出了挑战。在这里，我们提出了一个强大的光学和荧光在体内成像系统，IVIS光谱卡尺生命科学制造的收购。这将安装在HDFCRB屏障设施内，由HDFCCC临床前治疗核心（PTC）管理和运营，用于跟踪活体动物内标记肿瘤和细胞的生长和扩散。这项技术是任何癌症研究企业的重要组成部分，它使用高度复杂的硬件和软件，允许在几天、几周或几个月内对单个动物体内的多个荧光团进行成像。本文提出的各种应用包括在发育和患病组织中的细胞谱系和细胞命运分析的基础研究、细胞和转移途径的研究、体内特定致癌信号途径的研究、靶向特定分子途径用于癌症治疗的新药的临床前研究、优化成像方法和产生新的癌症生物标志物，可以迅速转化为临床肿瘤学实践。 公众相关性：该提案要求资金购买一个强大的体内成像系统，即由Caliper Life Sciences制造的IVIS Spectrum，该系统允许癌症研究人员在几天、几周或几个月的时间内跟踪活体动物体内肿瘤细胞的生长和扩散。因此，癌症研究科学家可以研究肿瘤生物学的基本机制，并在小鼠癌症模型中测试新的治疗方法，并开发疾病进展和治疗反应的新生物标志物。

DESCRIPTION (provided by applicant): In June 2009, the Helen Diller Family Comprehensive Cancer Center (HDFCCC) opened its new state-of-the-art Helen Diller Family Cancer Research Building (HDFCRB) located at the developing Mission Bay campus. The building currently houses over 30 PIs, undertaking basic, pre-clinical and translational cancer research. There is potential for a further 50% expansion by completion of an additional floor of laboratory and office space. The building has a modern vivarium, housing small animals within a barrier facility. The facility has the potential to house 13, 000 animal cages, each holding up to 5 adult mice per cage. This extra capacity will certainly be required by 2014, when the new 289 bed UCSF children's women's, and cancer hospital opens at the Mission Bay site, bringing with it the Mt. Zion clinical research teams. The opening of the HDFCRB has bought together a large number of cancer research scientists under one roof. However, the opening represents an expansion of cancer research at UCSF rather than complete consolidation. The size of the HDFCCC, with over 300 PIs distributed between three main sites within the city of San Francsico, presents a challenge in supplying the necessary infrastructure at each site, specifically of high end instruments to support basic, preclinical and translational research. Here we propose the acquisition of a powerful optical and fluorescence in vivo imaging system, the IVIS Spectrum manufactured by Caliper Life Sciences. This will be installed within the HDFCRB barrier facility, managed and operated by the HDFCCC Preclinical Therapeutics Core (PTC), for use in tracking the growth and spread of labeled tumors and cells within live animals. This technology is an essential component of any cancer research enterprise, using highly sophisticated hardware and software to allow the imaging of multiple fluorophores within a single animal over days, weeks or months. The various applications proposed herein include basic studies on cell lineage and cell fate analysis in developing and diseased tissues, investigation of cells and pathways of metastasis, investigation of specific oncogenic signal pathways in vivo, preclinical investigation of novel drugs targeted to specific molecular pathways for cancer therapy, optimization of imaging approaches and generation of novel cancer biomarkers that could be rapidly translated into clinical oncology practice. PUBLIC RELEVANCE: This proposal requests funds to purchase a powerful in vivo imaging system, the IVIS Spectrum manufactured by Caliper Life Sciences, which allows cancer researchers to track the growth and spread of tumors cells within live animals over periods of days, weeks or months. Cancer research scientists can therefore study basic mechanisms of tumor biology, as well as test novel therapeutics in mouse models of cancer, and develop new biomarkers of disease progression and responses to therapy.