IVIS Spectrum Imaging System

描述（由申请人提供）：2009年6月，海伦·迪勒家庭综合癌症中心（HDFCCC）在发展中的Mission Bay校区开设了新的最先进的海伦·迪勒家庭癌症研究大楼（HDFCRB）。该建筑目前容纳了30多名pi，从事基础，临床前和转化癌症研究。在实验室和办公空间的另一层完成后，有可能进一步扩大50%。该建筑有一个现代化的动物饲养场，在屏障设施内饲养小动物。该设施可容纳1.3万个动物笼子，每个笼子最多可容纳5只成年老鼠。到2014年，这个额外的容量肯定是需要的，届时，新的289张床位的加州大学旧金山分校儿童妇女和癌症医院将在使命湾开业，锡安山临床研究团队也将随之开业。HDFCRB的成立将大量的癌症研究科学家聚集在一起。然而，这一开放代表了加州大学旧金山分校癌症研究的扩展，而不是完全的整合。HDFCCC的规模有300多个pi分布在旧金山市的三个主要站点，这对每个站点提供必要的基础设施，特别是高端仪器来支持基础、临床前和转化研究提出了挑战。在这里，我们建议收购一个强大的光学和荧光体内成像系统，由卡柏生命科学公司生产的IVIS光谱。它将安装在HDFCRB屏障设施内，由HDFCCC临床前治疗核心（PTC）管理和操作，用于跟踪活体动物中标记肿瘤和细胞的生长和扩散。这项技术是任何癌症研究企业的重要组成部分，使用高度复杂的硬件和软件，可以在几天、几周或几个月内对单个动物体内的多个荧光团进行成像。本文提出的各种应用包括细胞谱系和细胞命运分析的基础研究，细胞和转移途径的研究，体内特定致癌信号通路的研究，针对癌症治疗特定分子途径的新药的临床前研究，成像方法的优化以及可快速转化为临床肿瘤学实践的新型癌症生物标志物的生成。

DESCRIPTION (provided by applicant): In June 2009, the Helen Diller Family Comprehensive Cancer Center (HDFCCC) opened its new state-of-the-art Helen Diller Family Cancer Research Building (HDFCRB) located at the developing Mission Bay campus. The building currently houses over 30 PIs, undertaking basic, pre-clinical and translational cancer research. There is potential for a further 50% expansion by completion of an additional floor of laboratory and office space. The building has a modern vivarium, housing small animals within a barrier facility. The facility has the potential to house 13, 000 animal cages, each holding up to 5 adult mice per cage. This extra capacity will certainly be required by 2014, when the new 289 bed UCSF children's women's, and cancer hospital opens at the Mission Bay site, bringing with it the Mt. Zion clinical research teams. The opening of the HDFCRB has bought together a large number of cancer research scientists under one roof. However, the opening represents an expansion of cancer research at UCSF rather than complete consolidation. The size of the HDFCCC, with over 300 PIs distributed between three main sites within the city of San Francsico, presents a challenge in supplying the necessary infrastructure at each site, specifically of high end instruments to support basic, preclinical and translational research. Here we propose the acquisition of a powerful optical and fluorescence in vivo imaging system, the IVIS Spectrum manufactured by Caliper Life Sciences. This will be installed within the HDFCRB barrier facility, managed and operated by the HDFCCC Preclinical Therapeutics Core (PTC), for use in tracking the growth and spread of labeled tumors and cells within live animals. This technology is an essential component of any cancer research enterprise, using highly sophisticated hardware and software to allow the imaging of multiple fluorophores within a single animal over days, weeks or months. The various applications proposed herein include basic studies on cell lineage and cell fate analysis in developing and diseased tissues, investigation of cells and pathways of metastasis, investigation of specific oncogenic signal pathways in vivo, preclinical investigation of novel drugs targeted to specific molecular pathways for cancer therapy, optimization of imaging approaches and generation of novel cancer biomarkers that could be rapidly translated into clinical oncology practice. PUBLIC RELEVANCE: This proposal requests funds to purchase a powerful in vivo imaging system, the IVIS Spectrum manufactured by Caliper Life Sciences, which allows cancer researchers to track the growth and spread of tumors cells within live animals over periods of days, weeks or months. Cancer research scientists can therefore study basic mechanisms of tumor biology, as well as test novel therapeutics in mouse models of cancer, and develop new biomarkers of disease progression and responses to therapy.