Becton Dickinson LSR II Analytical Flow Cytometer

Becton Dickinson LSR II 分析流式细胞仪

• 批准号: 8052222
• 负责人: Raphael A. Clynes
• 金额: $ 41.32万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-04 至 2012-04-03
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8052222
• 关键词: Accounting; Arterial Fatty Streak; Atherosclerosis; Biological Assay; Blood Cells; Cell Cycle; Cell Lineage; Cells; Communities; Complex; Core Facility; Cytometry; Detection; Development; Enzyme-Linked Immunosorbent Assay; Equipment; Flow Cytometry; Funding; Gene Expression; Graft Rejection; Head; Image Cytometry; Immunologist; Immunology; Inflammation; Inflammatory; Insulin-Dependent Diabetes Mellitus; Lasers; Maintenance; Malignant neoplasm of lung; Medicine; Myeloid Progenitor Cells; Population; Primary Cell Cultures; Proteins; Research; Research Personnel; Resolution; Resource Sharing; Sampling; Side; Signal Transduction; Sum; Suppressor-Effector T-Lymphocytes; Techniques; Therapeutic; Time; Tissues; Trafficking Protein Gene; Training; Translational Research; Transplantation Tolerance; United States National Institutes of Health; base; cancer transplantation; cytokine; high throughput analysis; immunoglobulin receptor; medical schools; multidisciplinary; release of sequestered calcium ion into cytoplasm; tool; translational study; user-friendly

DESCRIPTION (provided by applicant): Flow cytometry has become the essential tool for cell-based assays. Yet these versatile machines are technologically complex and the equipment expensive requiring shared use and the development of core facilities. Currently, the Department of Medicine (25 different NIH-funded users) accounts for the majority of the campus-wide use of flow cytometry. The increased demand from the Department of Medicine and elsewhere at CUMC has justified the development of an internally funded Imaging and Cytometry Core, headed by Dr. Clynes. This is part of a larger institutional effort to build translational research in medicine, including the newly created Center of Translational Immunology. This proposal, in particular, joins 8 multidisciplinary biomedical researchers (whose NIH funded research totals >$15,000,000/year), and seeks to integrate immunologists with specific expertise in flow cytometric techniques with investigators studying inflammation in cancer, the lung, and the atheromatous plaque. This will create a shared resource for use, training, maintenance and of the versatile LSR II flow cytometer throughout the School of Medicine at CUMC. Examples of NIH funded research that this Core will support include: stem cells/myeloid suppressor cells in cancer, transplantation rejection and tolerance, resolution of atherosclerosis and therapeutic blockade of immunoglobulin receptors in type I diabetes. The requested LSR II requires three optional and costly accessories to permit and expedite these translational studies. 1. The UV laser permits immunoreceptor signaling (calcium flux), side population detection, and cell cycle analysis. 2. The yellow-green laser broadens the cytometers capacity to detect a wide variety of red flourochromes, including Red Flourescence Protein, important for cell lineage analysis, gene expression and protein trafficking, dramatically increasing versatility. 3. The 96-well attachment will allow high throughput analysis of CFSE proliferation assays, multiple analytes, expediting assessment of inflammatory cytokines in large sample format of tissue cells/blood and cultured primary cells eliminating the need for time-consuming ELISA based assays. In sum the LSRII will catalyze the development of a versatile and user-friendly Cytometry Core for over 25 NIH-funded investigators in the Department of Medicine and will make manifest the integration of the Center of Translational Immunology within the general community of the School of Medicine.

描述(申请人提供)：流式细胞术已成为细胞分析的基本工具。然而，这些多功能机器技术复杂，设备昂贵，需要共享使用和开发核心设施。目前，在全校范围内使用的流式细胞仪中，医学系(25个不同的NIH资助用户)占了大部分。来自医学部和CUMC其他地方的需求增加，证明了开发由克莱恩斯博士领导的内部资助的成像和细胞学核心是合理的。这是建立医学转化研究的更大机构努力的一部分，其中包括新成立的转化免疫学中心。尤其是，这项提案加入了8名多学科生物医学研究人员(他们资助的研究总额为15,000,000美元/年)，并寻求将在流式细胞仪技术方面具有特定专业知识的免疫学家与研究癌症、肺部和动脉粥样硬化斑块的炎症的研究人员整合在一起。这将创建一个共享资源，供CUMC医学院使用、培训、维护和使用多功能LSR II流式细胞仪。该核心将支持的NIH资助的研究包括：癌症中的干细胞/髓系抑制细胞、移植排斥和耐受性、动脉粥样硬化的解决以及I型糖尿病免疫球蛋白受体的治疗阻断。所要求的LSR II需要三个可选且昂贵的附件来允许和加快这些翻译研究。1.紫外光激光允许免疫受体信号(钙通量)、侧群检测和细胞周期分析。2.黄绿激光扩大了细胞仪检测各种红色荧光蛋白的能力，包括对细胞谱系分析、基因表达和蛋白质运输至关重要的红色荧光蛋白，极大地增加了通用性。3.96孔附件将允许对CFSE增殖分析、多个分析物进行高通量分析，加快组织细胞/血液和培养的原代细胞的大样本格式中炎性细胞因子的评估，从而消除基于耗时的ELISA法分析的需要。总而言之，LSRII将促进为NIH资助的医学部25名研究人员开发一个多功能和用户友好的细胞学核心，并将体现转化免疫学中心在医学院普通社区中的整合。

项目成果

Macrochimerism in Intestinal Transplantation: Association With Lower Rejection Rates and Multivisceral Transplants, Without GVHD.

• DOI: 10.1111/ajt.13325
• 发表时间: 2015-10
• 期刊: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
• 作者: Zuber J;Rosen S;Shonts B;Sprangers B;Savage TM;Richman S;Yang S;Lau SP;DeWolf S;Farber D;Vlad G;Zorn E;Wong W;Emond J;Levin B;Martinez M;Kato T;Sykes M
• 通讯作者: Sykes M

Staphylococcus aureus small colony variants impair host immunity by activating host cell glycolysis and inducing necroptosis.

• DOI: 10.1038/s41564-019-0597-0
• 发表时间: 2020-01
• 期刊: Nature microbiology
• 影响因子: 28.3

Effect of Ex Vivo-Expanded Recipient Regulatory T Cells on Hematopoietic Chimerism and Kidney Allograft Tolerance Across MHC Barriers in Cynomolgus Macaques.

过体扩展的受体调节T细胞对cynomolgus猕猴中MHC障碍的造血嵌合体和肾脏同种异体耐受性的影响。

• DOI: 10.1097/tp.0000000000001559
• 发表时间: 2017-02
• 期刊: Transplantation
• 影响因子: 6.2
• 作者: Duran-Struuck R;Sondermeijer HP;Bühler L;Alonso-Guallart P;Zitsman J;Kato Y;Wu A;McMurchy AN;Woodland D;Griesemer A;Martinez M;Boskovic S;Kawai T;Cosimi AB;Yang YG;Hu Z;Wuu CS;Slate A;Mapara M;Baker S;Tokarz R;D'Agati V;Hammer S;Pereira M;Lipkin WI;Wekerle T;Levings M;Sykes M
• 通讯作者: Sykes M

T cell repertoire analysis suggests a prominent bystander response in human cardiac allograft vasculopathy.

• DOI: 10.1111/ajt.16333
• 发表时间: 2021-04
• 期刊: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
• 作者: Habal MV;Miller AMI;Rao S;Lin S;Obradovic A;Khosravi-Maharlooei M;See SB;Roy P;Shihab R;Ho SH;Marboe CC;Naka Y;Takeda K;Restaino S;Han A;Mancini D;Givertz M;Madsen JC;Sykes M;Addonizio LJ;Farr MA;Zorn E
• 通讯作者: Zorn E

Biased Generation and In Situ Activation of Lung Tissue-Resident Memory CD4 T Cells in the Pathogenesis of Allergic Asthma.

• DOI: 10.4049/jimmunol.1700257
• 发表时间: 2018-03-01
• 期刊: Journal of immunology (Baltimore, Md. : 1950)
• 作者: Turner DL;Goldklang M;Cvetkovski F;Paik D;Trischler J;Barahona J;Cao M;Dave R;Tanna N;D'Armiento JM;Farber DL
• 通讯作者: Farber DL