Acquisition of a DeltaVision OMX SR 3D SIM system for super-resolution imaging

采购用于超分辨率成像的 DeltaVision OMX SR 3D SIM 系统

• 批准号: 8051454
• 负责人: Alison Jane North
• 金额: $ 60万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8051454
• 关键词: Address; Alzheimer' s Disease; Area; Bacteria; Biological; Biomedical Research; Cell Nucleus; Cell division; Chemicals; Chromosome Segregation; Chromosomes; Color; Core Facility; DNA Damage; DNA biosynthesis; Dendritic Spines; Disease; Ensure; Funding; Genetic Materials; Genome Stability; Health; Hereditary Disease; Housing; Image; Learning; Length; Lighting; Location; Malignant Neoplasms; Mammalian Cell; Methods; Microscope; Molecular; Molecular Structure; Names; Neurons; New York; Nuclear Pore Complex Proteins; Organism; Parkinsonian Disorders; Pharmaceutical Preparations; Positioning Attribute; Proteins; Relative (related person); Request for Applications; Research; Research Personnel; Resolution; Resources; Role; Sampling; Schizophrenia; Site; Structure; System; Thick; Tumor Suppressor Proteins; Universities; Work; Yeasts; age related; base; cancer therapy; cell fixing; flexibility; improved; inhibitor/antagonist; pathogen; research study; segregation; telomere; three dimensional structure

DESCRIPTION (provided by applicant): This application requests funds to purchase an Applied Precision DeltaVision OMX SR 3D Structured Illumination system (3D-SIM) to be installed in the Bio-Imaging Resource Center (BIRC) at The Rockefeller University in New York. The DeltaVision OMX SR (DV OMX SR) is one of the first commercially available "super-resolution" microscopes that permit's researchers to resolve biological components or structures at double the resolution, in all three axes, of that which can be achieved using any conventional microscope. Moreover, this improved resolution can be achieved with multiple colors simultaneously and in any type of sample up to 10-20 micrometers thick, rendering this a very flexible approach that can now be applied to address a number of important questions in biomedical research that were hitherto impossible to answer. The researchers who will initially benefit from this system work on a broad array of organisms and health-related issues, including: the role of telomere length in repressing DNA damage in mammalian cells (relevant to cancer and age-related diseases); the molecular basis for ensuring accurate segregation of replicated chromosomes during mammalian cell division (evaluating chemical inhibitors of components as drugs for cancer therapy); structural changes in the dendritic spines of neurons that occur during learning as well as in disease (Alzheimer's, schizophrenia, Parkinsonism); understanding the structure of Streptococcal bacteria and other pathogens; understanding the organization and dynamics of both yeast and mammalian nuclear pore complex proteins (also implicated in proper chromosome segregation during cell division, and known to be potent tumor suppressors); and elucidating the ordering of DNA replication sites in the eukaryotic nucleus (accurate copying of the genetic material being crucial for genomic stability and to avoid somatic genetic diseases such as cancer). All of these questions will be approached by the method of using fluorescent tags to locate the relative positions of proteins, molecules or macromolecular structures within fixed cells. Although this approach has already been applied, in all cases, the inability of even the high-end microscopes in the BIRC to distinguish between neighboring components that are closer together than 200 nm in the xy axis, or 500 nm in the z-axis, has severely limited the questions we can ask. We show here that preliminary experiments using the DV OMX SR system provided new biological information in all of our research areas, opening up new lines of scientific enquiry that are presented below. The requested system will be housed in a large and well-supported core facility in a central location in New York, which offers access to numerous outside researchers as well as the named users and other RU investigators.

描述（由申请人提供）：本申请资金用于购买将安装在纽约洛克菲勒大学生物成像资源中心（BIRC）的Applied Precision DeltaVision OMX SR 3D结构化照明系统（3D-SIM）。DeltaVision OMX SR（DV OMX SR）是第一个商用的“超分辨率”显微镜之一，它允许研究人员在所有三个轴上以两倍于传统显微镜的分辨率分辨生物成分或结构。此外，这种改进的分辨率可以同时用多种颜色来实现，并且可以在任何类型的样品中达到10-20微米厚，使其成为一种非常灵活的方法，现在可以应用于解决生物医学研究中迄今为止无法回答的许多重要问题。最初将从该系统中受益的研究人员致力于广泛的生物体和健康相关问题，包括：端粒长度在抑制哺乳动物细胞DNA损伤中的作用（与癌症和年龄相关疾病有关）;确保哺乳动物细胞分裂期间复制染色体精确分离的分子基础（评估作为癌症治疗药物的成分的化学抑制剂）;在学习过程中以及在疾病中发生的神经元树突棘的结构变化（阿尔茨海默氏症，精神分裂症，帕金森氏症）;了解链球菌和其他病原体的结构;了解酵母和哺乳动物核孔复合物蛋白的组织和动力学（还涉及细胞分裂期间的适当染色体分离，并且已知是有效的肿瘤抑制剂）;并阐明了真核细胞核中DNA复制位点的顺序（遗传物质的精确复制对于基因组稳定性和避免体细胞遗传疾病如癌症是至关重要的）。所有这些问题都将通过使用荧光标记来定位固定细胞内蛋白质、分子或大分子结构的相对位置的方法来解决。虽然这种方法已经被应用，但在所有情况下，即使是BIRC中的高端显微镜也无法区分xy轴上距离小于200 nm或z轴上距离小于500 nm的相邻组件，这严重限制了我们可以提出的问题。我们在这里表明，使用DV OMX SR系统的初步实验在我们所有的研究领域提供了新的生物学信息，开辟了下面介绍的科学探究的新领域。所要求的系统将被安置在纽约中心位置的一个大型和支持良好的核心设施中，该设施为众多外部研究人员以及指定用户和其他RU调查人员提供访问权限。

项目成果

Distinct Spatiotemporal Dynamics of Peptidoglycan Synthesis between Mycobacterium smegmatis and Mycobacterium tuberculosis.

• DOI: 10.1128/mbio.01183-17
• 发表时间: 2017-09-12
• 期刊: mBio
• 影响因子: 6.4
• 作者: Botella H;Yang G;Ouerfelli O;Ehrt S;Nathan CF;Vaubourgeix J
• 通讯作者: Vaubourgeix J

Nup133 Is Required for Proper Nuclear Pore Basket Assembly and Dynamics in Embryonic Stem Cells.

Nup133 是胚胎干细胞中适当的核孔篮组装和动力学所必需的。

• DOI: 10.1016/j.celrep.2018.04.070
• 发表时间: 2018
• 期刊: Cell reports
• 影响因子: 8.8
• 作者: Souquet,Benoit;Freed,Ellen;Berto,Alessandro;Andric,Vedrana;Audugé,Nicolas;Reina-San-Martin,Bernardo;Lacy,Elizabeth;Doye,Valérie
• 通讯作者: Doye,Valérie