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Acquisition of a Leica SCN400 Slide Scanning System For Use In A Multi-User Facil

采购 Leica SCN400 载玻片扫描系统用于多用户设施

基本信息

批准号：
8052382
负责人：
PATRICIA A CONRAD
金额：
$ 37.72万
依托单位：
CASE WESTERN RESERVE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-04-01 至 2013-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8052382
关键词：
Applications Grants Award Basic Science Biological Biomedical Research Blood Vessels Cartilage Cells Clinical Colon Carcinoma Computer software Core Facility Coronary DNA Repair Development Developmental Process Devices Disease Equipment Fluorescent Probes Funding Genetic Germ Cells Grant Host Defense Mechanism Housing Image Imaging technology Immune Immune response In Situ Infection Institution Link Liver diseases Lung Malignant Neoplasms Mammography Measurement Mission Needle biopsy procedure Obesity Pattern Photons Process Quantitative Microscopy Radiation Research Research Project Grants Resolution Scanning Scientist Slide Soldier Structure System Technology Therapeutic Time Tissue Engineering Tissues Training United States National Institutes of Health Universities Whole Organism Wound Healing base cell behavior chemotherapy combat deafness design diet and cancer instrument instrumentation intravital microscopy research study tool

项目摘要

DESCRIPTION (provided by applicant): This application is for funds to purchase a Leica SCN400 Slide Scanner integrated with quantitation and stereology software from Visiopharm, and two remote workstations for offline analysis. The ability to automatically detect and capture in-focus, high resolution images of histochemical, immunohistochemical, or fluorescent probes on cells and tissues is paramount in contemporary basic and translational biomedical research. Quantitative microscopy becomes a powerful tool in both the research and clinical setting when a detailed analysis of tissue microstructure is essential to understand natural or pathological changes. Design- based stereological approaches are critical to avoid bias in biological measurement. The proposed system is very powerful as it integrates state-of-the-art scanning and image capture with accurate, unbiased quantitation. The acquired images will be analyzed offline, freeing the instrument for users needing access to its acquisition applications. The instrument will be housed in an imaging core facility in the Department of Genetics at Case Western Reserve University (CASE). The mission of the imaging core is to provide expertise, state-of-the-art instrumentation, and outstanding training and technical support to scientists at CASE and surrounding institutions. The proposed system will not only enhance the research capabilities of a university-supported core but directly impact the research of multiple NIH-funded grants including 14 R01s, 3 R21s, 2 P41s, and 1 P01, K99, R42, R02, P20 plus various clinical and training awards. The experiments utilizing the slide scanner technology outlined in this grant application cover all aspects of research from basic to clinical to translational, including fundamental processes in development (germ cell, blood vessel, lung) as well as developmental processes associated with disease (deafness, liver disease, cancers), host defense mechanisms against infections, coronary vasculature patterning, assessing the links between obesity, diet and colon cancer, the therapeutic impact of DNA-repair mechanisms after radiation and chemotherapy, infection and the immune response, tissue engineering of cartilage and vasculature, and the development of a portable device for the electrostimulation-induced healing of wounds suffered by soldiers in combat. In addition, several projects link the requested instrumentation to other imaging technologies, such as 2-photon intravital microscopy for in situ immune cell behavior, mammography for real-time guidance of needle biopsies, and cryo-imaging at multiple spatial scales from the whole organism to tissue structures to molecules. None of the proposed research projects can be accomplished with any currently available equipment on our campus or neighboring institutions. PUBLIC HEALTH RELEVANCE: NIH funded investigators at Case Western Reserve University request support for a new, integrated slide scanning system that will allow automated, quantitative, and stereological analysis of cell and tissue samples. The system will enable researchers to speed discoveries related specifically to Rift Valley fever virus, wound therapy, deafness, blindness, colon, lung, and liver cancers, and fundamental processes of development.

描述（由申请人提供）：此申请用于购买Leica SCN 400载玻片扫描仪（集成了Visiopharm的定量和体视学软件）和两个用于离线分析的远程工作站。自动检测和捕获细胞和组织上的组织化学、免疫组织化学或荧光探针的聚焦、高分辨率图像的能力在当代基础和转化生物医学研究中至关重要。定量显微镜成为一个强大的工具，在研究和临床设置时，组织微观结构的详细分析是必不可少的，以了解自然或病理变化。基于设计的体视学方法对于避免生物测量中的偏差至关重要。所提出的系统是非常强大的，因为它集成了最先进的扫描和图像捕获与准确，公正的定量。采集的图像将进行离线分析，从而使需要访问其采集应用程序的用户可以自由使用仪器。该仪器将被安置在凯斯西储大学（Case Western Reserve University）遗传学系的成像核心设施中。成像核心的使命是为CASE和周边机构的科学家提供专业知识、最先进的仪器以及出色的培训和技术支持。拟议的系统不仅将增强大学支持的核心的研究能力，而且直接影响多个NIH资助的赠款的研究，包括14个R01，3个R21，2个P41和1个P01，K99，R42，R02，P20以及各种临床和培训奖项。本申请中所述的利用载玻片扫描仪技术的实验涵盖了从基础到临床再到转化研究的各个方面，包括开发中的基本过程（生殖细胞、血管、肺）以及与疾病相关的发育过程（耳聋、肝病、癌症）、宿主对感染的防御机制、冠状血管系统模式、评估肥胖之间的联系，这些研究包括：饮食与结肠癌、放疗和化疗后DNA修复机制的治疗作用、感染与免疫反应、软骨和脉管系统的组织工程，以及开发一种便携式设备，用于电刺激诱导士兵在战斗中受伤的伤口愈合。此外，有几个项目将所要求的仪器与其他成像技术联系起来，例如用于原位免疫细胞行为的双光子活体显微镜，用于针活检实时指导的乳房X线摄影，以及从整个生物体到组织结构再到分子的多个空间尺度的冷冻成像。没有一个拟议的研究项目可以在我们的校园或邻近机构的任何现有设备完成。公共卫生相关性：美国国家卫生研究院资助的凯斯西储大学的研究人员要求支持一种新的集成载玻片扫描系统，该系统将允许对细胞和组织样本进行自动化，定量和体视学分析。该系统将使研究人员能够加快与裂谷热病毒，伤口治疗，耳聋，失明，结肠癌，肺癌和肝癌以及基本发育过程相关的发现。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Early pulmonary disease manifestations in cystic fibrosis mice.

DOI：
10.1016/j.jcf.2016.05.002
发表时间：
2016-11
期刊：
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
影响因子：
0
作者：
Darrah RJ;Mitchell AL;Campanaro CK;Barbato ES;Litman P;Sattar A;Hodges CA;Drumm ML;Jacono FJ
通讯作者：
Jacono FJ

Penile alterations at early stage of type 1 diabetes in rats.

DOI：
10.1590/s1677-5538.ibju.2016.0454
发表时间：
2017-07
期刊：
International braz j urol : official journal of the Brazilian Society of Urology
影响因子：
0
作者：
Tao M;Tasdemir C;Tasdemir S;Shahabi A;Liu G
通讯作者：
Liu G

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay.

使用 RNA 原位杂交测定对小鼠脑切片中的选择性前 mRNA 剪接进行定量分析。