Acquisition of a Leica SCN400 Slide Scanning System For Use In A Multi-User Facil

描述（由申请人提供）：本申请用于购买Visiopharm公司集成定量和立体软件的徕卡SCN400切片扫描仪，以及两个用于离线分析的远程工作站。在细胞和组织上自动检测和捕获组织化学、免疫组织化学或荧光探针的聚焦、高分辨率图像的能力在当代基础和转化生物医学研究中至关重要。当组织微观结构的详细分析对了解自然或病理变化至关重要时，定量显微镜在研究和临床环境中都成为一种强大的工具。在生物测量中，基于设计的立体学方法是避免偏倚的关键。所提出的系统是非常强大的，因为它集成了最先进的扫描和图像捕获与准确，无偏量化。采集的图像将离线分析，为需要访问其采集应用程序的用户释放仪器。该仪器将被安置在凯斯西储大学（Case）遗传学系的成像核心设施中。成像核心的使命是为CASE和周围机构的科学家提供专业知识、最先进的仪器、出色的培训和技术支持。拟议的系统不仅可以提高大学支持核心的研究能力，还可以直接影响nih资助的多个研究项目，包括14个r01、3个r21、2个p41、1个P01、K99、R42、R02、P20以及各种临床和培训奖励。本资助申请中概述的利用滑动扫描仪技术的实验涵盖了从基础到临床再到转化研究的各个方面，包括发育的基本过程（生殖细胞、血管、肺）以及与疾病相关的发育过程（耳聋、肝病、癌症）、宿主防御感染机制、冠状血管模式、评估肥胖、饮食和结肠癌之间的联系、放射和化疗后dna修复机制的治疗作用，感染和免疫反应，软骨和血管的组织工程，以及便携式电刺激装置的开发，用于士兵在战斗中遭受的伤口愈合。此外，一些项目将所要求的仪器与其他成像技术联系起来，例如用于原位免疫细胞行为的双光子活体显微镜，用于针活检实时指导的乳房x线照相术，以及从整个生物体到组织结构再到分子的多个空间尺度的冷冻成像。没有一个拟议的研究项目可以用我们校园或邻近机构现有的设备来完成。

DESCRIPTION (provided by applicant): This application is for funds to purchase a Leica SCN400 Slide Scanner integrated with quantitation and stereology software from Visiopharm, and two remote workstations for offline analysis. The ability to automatically detect and capture in-focus, high resolution images of histochemical, immunohistochemical, or fluorescent probes on cells and tissues is paramount in contemporary basic and translational biomedical research. Quantitative microscopy becomes a powerful tool in both the research and clinical setting when a detailed analysis of tissue microstructure is essential to understand natural or pathological changes. Design- based stereological approaches are critical to avoid bias in biological measurement. The proposed system is very powerful as it integrates state-of-the-art scanning and image capture with accurate, unbiased quantitation. The acquired images will be analyzed offline, freeing the instrument for users needing access to its acquisition applications. The instrument will be housed in an imaging core facility in the Department of Genetics at Case Western Reserve University (CASE). The mission of the imaging core is to provide expertise, state-of-the-art instrumentation, and outstanding training and technical support to scientists at CASE and surrounding institutions. The proposed system will not only enhance the research capabilities of a university-supported core but directly impact the research of multiple NIH-funded grants including 14 R01s, 3 R21s, 2 P41s, and 1 P01, K99, R42, R02, P20 plus various clinical and training awards. The experiments utilizing the slide scanner technology outlined in this grant application cover all aspects of research from basic to clinical to translational, including fundamental processes in development (germ cell, blood vessel, lung) as well as developmental processes associated with disease (deafness, liver disease, cancers), host defense mechanisms against infections, coronary vasculature patterning, assessing the links between obesity, diet and colon cancer, the therapeutic impact of DNA-repair mechanisms after radiation and chemotherapy, infection and the immune response, tissue engineering of cartilage and vasculature, and the development of a portable device for the electrostimulation-induced healing of wounds suffered by soldiers in combat. In addition, several projects link the requested instrumentation to other imaging technologies, such as 2-photon intravital microscopy for in situ immune cell behavior, mammography for real-time guidance of needle biopsies, and cryo-imaging at multiple spatial scales from the whole organism to tissue structures to molecules. None of the proposed research projects can be accomplished with any currently available equipment on our campus or neighboring institutions. PUBLIC HEALTH RELEVANCE: NIH funded investigators at Case Western Reserve University request support for a new, integrated slide scanning system that will allow automated, quantitative, and stereological analysis of cell and tissue samples. The system will enable researchers to speed discoveries related specifically to Rift Valley fever virus, wound therapy, deafness, blindness, colon, lung, and liver cancers, and fundamental processes of development.