Novel Optrode Devices for Neuroscientists: Packaging and Waveguide Solutions to M

描述（由申请人提供）：本申请的目的是为神经科学家开发先进的光极解决方案，以实现光遗传技术的潜力-实现高度特异性的神经回路控制。一旦开发出来，神经科学家的光学刺激实验选项将增长到两个维度，对记录部位的位置没有限制。我们的方法开发（i）实用，但目前困扰用户的包装问题的新的解决方案，和（ii）定制波导能够区域特定的照明，没有电气伪影，并模块化集成到任何现有的NeuroNexus记录阵列。作为晶圆级集成的替代方案，我们设计了一种方法，通过提高产量同时增加设计选择来降低成本。该项目将进一步发展光遗传学技术，该技术已显示出作为工具的良好前景，该工具允许在明确定义的神经元群体中对活性进行时间上精确的、非侵入性的控制。这种对神经放电的控制程度允许在电路动态背景下具体监测时间活动模式，了解可塑性引起的变化以及对行为和外部线索的反应，这对于研究疾病模型也至关重要。 公共卫生相关性：本申请的目的是为神经科学家开发先进的光极解决方案，以实现光遗传技术的潜力，实现高度特异性的神经回路控制。一旦开发出来，神经科学家的光学刺激实验选项将增长到两个维度，对记录部位的位置没有限制。我们的方法开发（i）实用，但目前困扰用户的包装问题的新的解决方案，和（ii）定制波导能够区域特定的照明，没有电气伪影，并模块化集成到任何现有的NeuroNexus记录阵列。作为晶圆级集成的替代方案，我们设计了一种方法，通过提高产量同时增加设计选择来降低成本。该项目将进一步发展光遗传学技术，该技术已显示出作为工具的良好前景，该工具允许在明确定义的神经元群体中对活性进行时间上精确的、非侵入性的控制。这种对神经放电的控制程度允许在电路动态的背景下对时间活动模式进行特定监测，理解由于可塑性引起的变化以及对行为和外部线索的反应，这对于研究疾病模型也至关重要。

DESCRIPTION (provided by applicant): The objective of this application is to develop advanced optrode solutions for neuroscientists that fulfill the potential of optogenetic technology-achieving highly specific neural circuit control. Once developed, the neuroscientist's experimental options for optical stimulation will grow to two dimensions with no limits on the recording site placement. Our approach develops (i) practical, yet novel solutions for the packaging issues currently plaguing users, and (ii) custom waveguides capable of region-specific illumination with no electrical artifact, and is modularly integrated onto any existing NeuroNexus recording array. As an alternative to wafer- level integration, we have devised an approach that lowers cost by improving yield while increasing design options. This project will further optogenic techniques, which have shown excellent promise as tools that allow temporally precise, non-invasive control of activity in well- defined neuronal populations. This degree of control over neural firing allows specific monitoring of temporal activity patterns in the context of circuit dynamics, understanding changes due to plasticity, and responses to behavior and external cues, which is critically important for studying disease models as well. PUBLIC HEALTH RELEVANCE: The objective of this application is to develop advanced optrode solutions for neuroscientists that fulfill the potential of optogenetic technology-achieving highly specific neural circuit control. Once developed, the neuroscientist's experimental options for optical stimulation will grow to two dimensions with no limits on the recording site placement. Our approach develops (i) practical, yet novel solutions for the packaging issues currently plaguing users, and (ii) custom waveguides capable of region-specific illumination with no electrical artifact, and is modularly integrated onto any existing NeuroNexus recording array. As an alternative to wafer- level integration, we have devised an approach that lowers cost by improving yield while increasing design options. This project will further optogenic techniques, which have shown excellent promise as tools that allow temporally precise, non-invasive control of activity in well- defined neuronal populations. This degree of control over neural firing allows specific monitoring of temporal activity patterns in the context of circuit dynamics, understanding changes due to plasticity, and responses to behavior and external cues, which is critically important for studying disease models as well.