Mosquito larval immunity and its impact on gut microbiota and malaria competence

描述（由申请人提供）：疟疾媒介能力主要由肠道微环境中蚊子肠道微生物群、疟原虫和宿主免疫力之间的生态相互作用决定。蚊子 免疫研究主要集中在成年人身上。幼虫-病原体相互作用是 是蚊子免疫系统进化过程中不可替代的驱动力。然而，在这方面， 对幼虫的免疫结构及其与肠道微生物植物群的相互作用知之甚少。在这 本课题拟利用球形芽孢杆菌进行蚊幼虫免疫研究 响应，并通过使用基于微阵列的全局 表达谱分析幼虫免疫激活对中华绒螯蟹肠道菌群动态的影响 将通过高通量分析肠道细菌群落来评估幼虫和成虫 焦磷酸测序细菌16S rDNA标记物。这些参数对疟疾的影响 还将评估敏感性。该项目将产生数据， 了解幼虫免疫及其对蚊子肠道生态系统的影响。

DESCRIPTION (provided by applicant): Malaria vector competence is largely determined by ecological interactions among mosquito gut microbiota, Plasmodium, and host immunity in the context of gut microenvironment. Mosquito immunity studies have been mainly focusing on the adults. Larva-pathogen interaction is an unignorable driving force in shaping mosquito immune system during the evolution. However, little is known about larva immune structure and its interaction with gut microbial flora. In this project we propose to use larvicidal Bacillus sphaericus to study mosquito larval immune response, and characterize larval immune architecture by using microarray based global expression profiling. The impact of larval immune activation on the dynamics of gut microbiota in both larvae and adults will be assessed by profiling gut bacterial community via high throughput pyrosequencing bacterial 16S rDNA marker. The influence of these parameters on the malaria susceptibility will also be evaluated. The project will generate data towards a better understanding of larval immunity and its impact on mosquito gut ecosystem.