TRANSGENIC MONKEY MODEL OF HUNTINGTON?S DISEASE - SUPPLEMENT

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在这项补充研究中，我们将在两个预算年度内每隔六个月监测这些HD猴子的外周血液的基因表达和miRNA表达谱。虽然部分HD和WT猴的外周血液样本已经在6个月和12个月大时采集，但我们建议在18、24、30和36个月龄时继续采集血液，如建议的研究所述。一旦基因组和miRNA图谱研究的平台建立起来，外周血液的持续监测将与父母拨款支持的其他原始HD猴子的纵向研究平行进行，包括认知、行为评估和非侵入性成像。我们推测，对外周血的分析可以揭示独特的差异基因表达模式和独特的miRNA表达谱，这可以作为HD生物学异常状况的指标，从而可以识别HD进展的生物标志物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In this supplemental study, we will monitor the gene expression and miRNA expression profile of the peripheral blood of these HD monkeys at six months intervals throughout the two budgeted years. While peripheral blood samples have already been collected from some of the HD and WT-monkeys at six and twelve month of age, we propose that continuing blood collection occur at 18, 24, 30 and 36 month of age as described in the proposed study. Once the platform of the genome and miRNA profiling studies is established, continued monitoring of peripheral blood will be performed parallel to the other original HD monkeys' longitudinal studies supported by the parent grant, which include cognitive, behavior evaluation, and noninvasive imaging. We hypothesize that analysis of peripheral blood could reveal unique differential gene expression patterns and unique expression profiles of miRNA, which could be used as an indicator of abnormal biological conditions in HD, thus biomarkers of HD progression could be identified.