Functions of Branched-Chain Fatty Acids in the Nematode Worm C. elegans

支链脂肪酸在线虫蠕虫中的功能 秀丽隐杆线虫

• 批准号: 8143302
• 负责人: Max Louis Cohen
• 金额: $ 3.14万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8143302
• 关键词: ATP phosphohydrolase; Animal Model; Animals; Binding; Biological Assay; Caenorhabditis; Caenorhabditis elegans; Carbon; Cells; Cellular Membrane; Co-Immunoprecipitations; Defect; Dependence; Development; Diabetes Mellitus; Embryo; Embryonic Development; Experimental Genetics; Fatty Acids; Genes; Genetic; Genetic Screening; Growth; Laboratories; Leucine; Life; Lipids; Maps; Membrane; Metabolic; Metabolic Diseases; Metabolism; Mutation; Nematoda; Nutritional; Obesity; Organism; Palmitates; Palmitic Acids; Pathway interactions; Play; Role; Second Messenger Systems; Signal Pathway; Signal Transduction; Structure; Supplementation; Time; Tissues; Vertebral column; Work; branched chain fatty acid; gene cloning; hatching; insight; loss of function; mutant; novel; research study; second messenger

DESCRIPTION (provided by applicant): Fatty acids are an important class of lipid molecules, one of the four main building-blocks for all living tissues. Our laboratory has recently identified the branched-chain fatty acid 15-methyl hexadecanoic acid, also known as C17ISO, as required for post-embryonic growth in the nematode worm C. elegans. In the absence of this molecule, which is a methlyated palmitate and probably a catabolite of leucine, worms are unable to grow after hatching, but this arrest can be suppressed by supplementation with C17ISO. C17ISO has been implicated in the coordinated sensing of nutritional and metabolic status to allow for larval growth, but the mechanism of its action is unknown. Through genetic screens we have identified several complete-suppressor strains and a partial-suppressor strain, which suggests that C17ISO may be part of a lipid-signaling or -metabolism pathway that is essential for worm development. I propose to (1) identify the genes involved with this putative signaling pathway and (2) characterize the mechanism of the already identified partial-suppressor mutant strain.

描述（由申请人提供）：脂肪酸是一类重要的脂质分子，是所有活组织的四种主要结构单元之一。我们的实验室最近已经确定了支链脂肪酸15-甲基十六烷酸，也被称为C17 ISO，作为所需的后胚胎生长的线虫蠕虫C。优美的在没有这种分子的情况下，它是一种甲基化棕榈酸酯，可能是亮氨酸的分解代谢产物，蠕虫在孵化后无法生长，但这种停滞可以通过补充C17 ISO来抑制。C17 ISO与营养和代谢状态的协调感测有关，以允许幼虫生长，但其作用机制尚不清楚。通过遗传筛选，我们已经确定了几个完全抑制菌株和部分抑制菌株，这表明C17 ISO可能是一个脂质信号或代谢途径的一部分，这是必不可少的蠕虫发展。我建议（1）确定参与这一假定的信号通路的基因和（2）表征已经确定的部分抑制突变株的机制。