"Glycosciences Skills Development "

“糖科学技能发展”

• 批准号: 8183699
• 负责人: GERALD Warren HART
• 金额: $ 5.71万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8183699
• 关键词: Acute; Address; Biology; Biomedical Research; Blood; Blood Cells; Carbohydrates; Cardiovascular Diseases; Chronic; Communities; Complex; Development; Disease; Educational process of instructing; Exercise; Glycobiology; Glycoconjugates; Goals; Heart; Hour; Individual; Institutes; Instruction; Knowledge; Laboratories; Language; Lipids; Lung; Methods; Mission; Molecular; National Heart, Lung, and Blood Institute; Phase; Play; Polysaccharides; Post-Translational Protein Processing; Postdoctoral Fellow; Proteins; Qualifying; Reading; Research; Role; Structure; Students; Time; Training; United States National Institutes of Health; Work; carbohydrate binding protein; design; enzyme biosynthesis; extracellular; graduate student; human disease; lectures; meetings; next generation; programs; skills; skills training

Nearly all of the diseases targeted by NHLBI directly involve glycoconjugates. However, there is both an acute lack of individuals with the knowledge and skills necessary to study glycoconjugates at the structure/function level. In fact, the complex extracellular and the simple, but dynamic, intracellular saccharides that are attached to proteins and lipids, do indeed play fundamental roles in many aspects of cardiovascular disease, lung biology and in the functions of all types of blood cells. We have assembled a unique team of experts in most aspects of glycosciences to not only carry-out the research goals of this PEG, but also to train the next generation of glycoscientists in the unique language and skills required to study glycoconjugates. Training the next generation of glycobiologists in the varied and complex methods and approaches that are required to study lycoconjugates is indeed critical to the next phase of biomedical research, which will be mostly focused on post-translational modifications. We have designed a thorough and comprehensive Glycosciences Skills Development Course, which in the first half will first teach our Fellows and students the basics of glycosciences in about twenty-eight 2h lectures (56 contact hours), discussions and reading (-10 weeks). The second half of the Skills Development Course will consist of nineteen hands-on laboratory exercises which will directly instruct our Fellows in the major methods of Glycosciences. These laboratory exercises will meet 3 times per week (M,W,F) for approximately 5h each (-95 contact hours). We strongly feel that only by such a comprehensive and hands-on approach can we begin to adequately prepare our Fellows to become leaders In glycoscience research. Part I of the Course starts with an overview of mammalian glycobiology, presents detailed information on each class of glycoconjugate, next discusses their biosynthesis, the enzymes controlling their structures, and ends with a discussion of carbohydrate binding proteins. Part II starts with an overview of glycan functions and then discusses their roles in human disease, culminating with a special lecture and round table discussion on their roles in cardiovascular disease. Part III starts with a lecture on the principles of carbohydrate structural determination and discusses the approaches for detailed analyses of various glycan types. Part IV is the hands-on laboratory course in which each Project or Core leader (with the help of their laboratory staff) will instruct the PEG Fellows in many of the methods used to analyze glycans. The Course will be offered every other year during the Program and will be limited to -10 students with PEG Fellows having priority. While a lot of work, this Core is the most efficient way to generate qualified glycobiologists!

NHLBI针对的几乎所有疾病都直接涉及糖共轭化合物。然而，缺乏在结构/功能水平上研究糖共轭所需的知识和技能的个人。事实上，复杂的胞外和简单但动态的胞内糖附在蛋白质和脂类上，确实在心血管疾病、肺部生物学和所有类型的血细胞的功能的许多方面发挥着基础作用。我们已经组建了一个独特的专家团队，在糖科学的大多数方面，不仅是为了实现这一研究目标 这不仅是为了培养下一代的糖科学专家，也是为了培养他们学习糖共轭化合物所需的独特语言和技能。对下一代糖生物学家进行研究糖结合物所需的各种复杂方法和途径的培训确实对下一阶段的生物医学研究至关重要，下一阶段的生物医学研究将主要集中于翻译后修饰。 我们已经设计了一门全面而全面的糖科学技能发展课程，在前半部分，我们将首先用大约28小时的讲座(56个接触小时)、讨论和阅读(-10周)向我们的研究员和学生传授糖科学的基础知识。技能发展课程的后半部分将包括19个动手实验室练习，这些练习将直接指导我们的研究员 糖科学的主要方法。这些实验室练习每周进行3次(男、女、女)，每次约5小时(-95个接触时间)。我们强烈认为，只有通过这种全面和亲力亲为的方法，我们才能开始为我们的研究员做好充分的准备，使他们成为糖科学研究的领导者。 本课程的第一部分首先概述了哺乳动物的糖生物学，介绍了每一类糖的详细信息，然后讨论了它们的生物合成，控制其结构的酶，最后讨论了碳水化合物结合蛋白。第二部分首先概述了多糖的功能，然后讨论了它们在人类疾病中的作用，最后以一场关于它们在心血管疾病中的作用的特别讲座和圆桌讨论告终。第三部分从碳水化合物结构测定原理的讲座开始，讨论了各种糖类的详细分析方法。第四部分是动手实验室课程，在该课程中，每个项目或核心负责人(在其实验室工作人员的帮助下)将指导聚乙二醇组研究员许多用于分析多糖的方法。本课程每隔一年开设一次，限于10名学生，优先考虑聚乙二醇制奖学金获得者。虽然有大量的工作，但这个核心是产生合格的糖生物学家的最有效的方式！