"Glycosciences Skills Development "

“糖科学技能发展”

• 批准号: 8183699
• 负责人: GERALD Warren HART
• 金额: $ 5.71万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8183699
• 关键词: Acute; Address; Biology; Biomedical Research; Blood; Blood Cells; Carbohydrates; Cardiovascular Diseases; Chronic; Communities; Complex; Development; Disease; Educational process of instructing; Exercise; Glycobiology; Glycoconjugates; Goals; Heart; Hour; Individual; Institutes; Instruction; Knowledge; Laboratories; Language; Lipids; Lung; Methods; Mission; Molecular; National Heart, Lung, and Blood Institute; Phase; Play; Polysaccharides; Post-Translational Protein Processing; Postdoctoral Fellow; Proteins; Qualifying; Reading; Research; Role; Structure; Students; Time; Training; United States National Institutes of Health; Work; carbohydrate binding protein; design; enzyme biosynthesis; extracellular; graduate student; human disease; lectures; meetings; next generation; programs; skills; skills training

Nearly all of the diseases targeted by NHLBI directly involve glycoconjugates. However, there is both an acute lack of individuals with the knowledge and skills necessary to study glycoconjugates at the structure/function level. In fact, the complex extracellular and the simple, but dynamic, intracellular saccharides that are attached to proteins and lipids, do indeed play fundamental roles in many aspects of cardiovascular disease, lung biology and in the functions of all types of blood cells. We have assembled a unique team of experts in most aspects of glycosciences to not only carry-out the research goals of this PEG, but also to train the next generation of glycoscientists in the unique language and skills required to study glycoconjugates. Training the next generation of glycobiologists in the varied and complex methods and approaches that are required to study lycoconjugates is indeed critical to the next phase of biomedical research, which will be mostly focused on post-translational modifications. We have designed a thorough and comprehensive Glycosciences Skills Development Course, which in the first half will first teach our Fellows and students the basics of glycosciences in about twenty-eight 2h lectures (56 contact hours), discussions and reading (-10 weeks). The second half of the Skills Development Course will consist of nineteen hands-on laboratory exercises which will directly instruct our Fellows in the major methods of Glycosciences. These laboratory exercises will meet 3 times per week (M,W,F) for approximately 5h each (-95 contact hours). We strongly feel that only by such a comprehensive and hands-on approach can we begin to adequately prepare our Fellows to become leaders In glycoscience research. Part I of the Course starts with an overview of mammalian glycobiology, presents detailed information on each class of glycoconjugate, next discusses their biosynthesis, the enzymes controlling their structures, and ends with a discussion of carbohydrate binding proteins. Part II starts with an overview of glycan functions and then discusses their roles in human disease, culminating with a special lecture and round table discussion on their roles in cardiovascular disease. Part III starts with a lecture on the principles of carbohydrate structural determination and discusses the approaches for detailed analyses of various glycan types. Part IV is the hands-on laboratory course in which each Project or Core leader (with the help of their laboratory staff) will instruct the PEG Fellows in many of the methods used to analyze glycans. The Course will be offered every other year during the Program and will be limited to -10 students with PEG Fellows having priority. While a lot of work, this Core is the most efficient way to generate qualified glycobiologists!

几乎所有NHLBI靶向的疾病都直接涉及糖缀合物。然而，有一个严重缺乏个人的知识和技能，必要的研究糖缀合物的结构/功能水平。事实上，与蛋白质和脂质连接的复杂的细胞外和简单但动态的细胞内酶确实在心血管疾病、肺生物学和所有类型血细胞的功能的许多方面发挥着重要作用。我们已经组建了一个独特的专家团队，在糖科学的大多数方面，不仅开展了这项研究的目标， PEG，但也培养下一代的糖科学家在独特的语言和技能所需的研究糖缀合物。在研究糖缀合物所需的各种复杂的方法和途径中培训下一代糖生物学家对于下一阶段的生物医学研究确实至关重要，这将主要集中在翻译后修饰上。 我们设计了一个全面的糖科学技能发展课程，在上半年，我们的研究员和学生将首先在大约28个2小时的讲座（56个接触小时），讨论和阅读（-10周）中教授糖科学的基础知识。技能发展课程的后半部分将包括十九个动手实验室练习，这些练习将直接指导我们的研究员 糖科学的主要方法。这些实验室练习将每周进行3次（M、W、F），每次约5小时（约95个接触小时）。我们强烈认为，只有通过这种全面和动手的方法，我们才能开始充分准备我们的研究员成为领导者在糖科学研究。 课程的第一部分首先概述哺乳动物糖生物学，介绍每类糖缀合物的详细信息，然后讨论它们的生物合成，控制它们结构的酶，最后讨论碳水化合物结合蛋白。第二部分从聚糖功能的概述开始，然后讨论它们在人类疾病中的作用，最后是关于它们在心血管疾病中的作用的特别讲座和圆桌讨论。第三部分首先介绍了碳水化合物结构测定的原理，并讨论了各种聚糖类型的详细分析方法。第四部分是实验室实践课程，每个项目或核心领导人（在实验室工作人员的帮助下）将指导PEG研究员使用许多用于分析聚糖的方法。该课程将在计划期间每隔一年提供一次，并将限于10名学生，PEG研究员优先。虽然有很多工作，但这个核心是产生合格糖生物学家的最有效方法！