The application of modern Synthetic Biology techniques to address the production of nutritional and industrial speciality chemicals

应用现代合成生物学技术来解决营养和工业特种化学品的生产

• 批准号: 2289306
• 金额: --
• 依托单位: Royal Holloway University of London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2289306
• 关键词: application; modern; Synthetic; Biology; techniques

Isoprenoids, also known as terpenoids represent the largest and oldest class of natural products documented. In crop plants intense efforts have been made to increase nutritional isoprenoids such as carotenoids (b-carotene provitamin A) and tocopherols (vitamin E) as a means of nutritional enhancement. Commercially isoprenoids are used in cosmetics and fragrances, and as colorants and nutritional supplements in foods and feeds. The present production platforms are either plants and/or microorganisms. Biosynthetically all isoprenoids are related via a common five carbon building block (isopentenyl pyrophosphate; IPP). Different isoprenoid classes can utilise the same isoprenoid precursors, which are preferentially used or channeled at different developmental stages. Through the creation of enzyme fusions a means of altering/optimising metabolite challenging can been achieved. These fusions which utilise flexible peptide linkers have the potential to optimise the flow of precursors from one class of isoprenoid into the desired products. In effect create an artificial metabolon. Proof of concept work has proven the potential of the system both in terms of enzymatic function and high throughput screening. In the proposed project fusions between isoprenoid enzymes metabolising geranylgeranyl (C20) or farnesyl (C15) diphosphate will be created and tested in plants and yeasts.

类异戊二烯，也称为萜类化合物，是有记载的最大和最古老的一类天然产物。在作物植物中，已经进行了大量的努力来增加营养类异戊二烯，例如类胡萝卜素（b-胡萝卜素原维生素A）和生育酚（维生素E），作为营养增强的手段。商业上，类异戊二烯用于化妆品和香料中，并作为食品和饲料中的着色剂和营养补充剂。目前的生产平台是植物和/或微生物。生物合成所有类异戊二烯通过共同的五碳结构单元（异戊烯焦磷酸; IPP）相关。不同的类异戊二烯类可以利用相同的类异戊二烯前体，其在不同的发育阶段优先使用或引导。通过创建酶融合物，可以实现改变/优化代谢物挑战的手段。这些利用柔性肽接头的融合物具有优化前体从一类类异戊二烯到所需产物的流动的潜力。实际上创造了一个人造代谢子。概念验证工作已经证明了该系统在酶功能和高通量筛选方面的潜力。在拟议的项目中，将在植物和酵母中创建和测试代谢香叶基香叶基（C20）或法呢基（C15）二磷酸的类异戊二烯酶之间的融合。