CORE D: DYNAMIC IMAGING FACILITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 核心动态成像：为Cobre计划提供动态成像和分析服务。Core D提供设备和专业知识来进行钙离子/视频成像实验，并协助分析任何类型的成像数据。核心D有两个专门的钙成像室，每个都有一个完整的设置，以获取结合电生理和/或张力记录的钙电影。可以在上面进行实验 在室温或生理温度下，单个细胞或整个组织中。此外，其他装置还允许对完整或解剖组织的运动进行视频记录。为了以高效的方式分析数据，核心D具有中央文件服务器 以及用于长期存储和快速访问数据文件的RAID存储池。核心D的一个重要组成部分是能够对数据进行量身定做的分析，以回答PIS提出的具体问题。可使用内部分析程序(Volumeter G6a)进行所有分析，包括：运动稳定和 分析、时空地图分析、活动的协调/关联和三维重建。Core D为每个用户提供广泛的图像分析培训和优化实验的建议。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Core D- Dynamic imaging: provides dynamic imaging and analysis services tothe COBRE program. CORE D provides equipment and expertise to carry outCa2+/video imaging experiments and assists in the analysis of any type ofimaging data. CORE D has 2 dedicated Ca2+ imaging rooms, each with acomplete setup for acquiring Ca2+ movies combined with either electrophysiology and/or tension recordings. Experiments can be performed on single cells or in whole tissues at room or physiological temperatures. In addition, other setups allow video recordings of movements in intact or dissected tissues. In order to analyze data in an efficient manner, CORE D has a central file server and RAID storage pool for long term storage and quick access to data files. An important component of CORE D is the ability to tailor analysis of data to answer specific questions posed by the PIS. An in-house analysis program (Volumetry G6a) is available that is used for all analysis including: motion stabilization and analysis, spatio-temporal map analysis, coordination/correlation of activities and 3D reconstruction. CORE D provides each user with extensive image analysis training and recommendations for optimizing experiments.