CORE D: DYNAMIC IMAGING FACILITY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 动态成像：为COBRE计划提供动态成像和分析服务。CORE D提供设备和专业知识来进行Ca 2 +/视频成像实验，并协助分析任何类型的成像数据。CORE D有2个专用的Ca 2+成像室，每个室都有完整的设置，用于采集Ca 2+电影，并结合电生理和/或张力记录。实验可以在 在室温或生理温度下在单个细胞或整个组织中。此外，其他设置允许视频记录完整或解剖组织中的运动。为了以有效的方式分析数据，CORE D具有中央文件服务器 和RAID存储池，用于长期存储和快速访问数据文件。CORE D的一个重要组成部分是能够定制数据分析，以回答PIS提出的具体问题。可使用内部分析程序（容积G6 a）进行所有分析，包括：运动稳定性和 分析、时空地图分析、活动协调/关联和3D重建。CORE D为每个用户提供广泛的图像分析培训和优化实验的建议。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Core D- Dynamic imaging: provides dynamic imaging and analysis services tothe COBRE program. CORE D provides equipment and expertise to carry outCa2+/video imaging experiments and assists in the analysis of any type ofimaging data. CORE D has 2 dedicated Ca2+ imaging rooms, each with acomplete setup for acquiring Ca2+ movies combined with either electrophysiology and/or tension recordings. Experiments can be performed on single cells or in whole tissues at room or physiological temperatures. In addition, other setups allow video recordings of movements in intact or dissected tissues. In order to analyze data in an efficient manner, CORE D has a central file server and RAID storage pool for long term storage and quick access to data files. An important component of CORE D is the ability to tailor analysis of data to answer specific questions posed by the PIS. An in-house analysis program (Volumetry G6a) is available that is used for all analysis including: motion stabilization and analysis, spatio-temporal map analysis, coordination/correlation of activities and 3D reconstruction. CORE D provides each user with extensive image analysis training and recommendations for optimizing experiments.