CORE D: DYNAMIC IMAGING FACILITY

核心 D：动态成像设施

• 批准号: 8360524
• 负责人: Terence Keith Smith
• 金额: $ 16.69万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360524
• 关键词: Cells; Centers of Research Excellence; Data; Data Analyses; Data Files; Electrophysiology (science); Equipment; Funding; Grant; Housing; Image; Image Analysis; Maps; Motion; Movement; National Center for Research Resources; Physiological; Principal Investigator; Recommendation; Research; Research Infrastructure; Resources; Services; Smooth Muscle; Source; Temperature; Tissues; Training; United States National Institutes of Health; Video Recording; cost; movie; programs; reconstruction; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Core D- Dynamic imaging: provides dynamic imaging and analysis services tothe COBRE program. CORE D provides equipment and expertise to carry outCa2+/video imaging experiments and assists in the analysis of any type ofimaging data. CORE D has 2 dedicated Ca2+ imaging rooms, each with acomplete setup for acquiring Ca2+ movies combined with either electrophysiology and/or tension recordings. Experiments can be performed on single cells or in whole tissues at room or physiological temperatures. In addition, other setups allow video recordings of movements in intact or dissected tissues. In order to analyze data in an efficient manner, CORE D has a central file server and RAID storage pool for long term storage and quick access to data files. An important component of CORE D is the ability to tailor analysis of data to answer specific questions posed by the PIS. An in-house analysis program (Volumetry G6a) is available that is used for all analysis including: motion stabilization and analysis, spatio-temporal map analysis, coordination/correlation of activities and 3D reconstruction. CORE D provides each user with extensive image analysis training and recommendations for optimizing experiments.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 动态成像：为COBRE计划提供动态成像和分析服务。CORE D提供设备和专业知识来进行Ca 2 +/视频成像实验，并协助分析任何类型的成像数据。CORE D有2个专用的Ca 2+成像室，每个室都有完整的设置，用于采集Ca 2+电影，并结合电生理和/或张力记录。实验可以在 在室温或生理温度下在单个细胞或整个组织中。此外，其他设置允许视频记录完整或解剖组织中的运动。为了以有效的方式分析数据，CORE D具有中央文件服务器 和RAID存储池，用于长期存储和快速访问数据文件。CORE D的一个重要组成部分是能够定制数据分析，以回答PIS提出的具体问题。可使用内部分析程序（容积G6 a）进行所有分析，包括：运动稳定性和 分析、时空地图分析、活动协调/关联和3D重建。CORE D为每个用户提供广泛的图像分析培训和优化实验的建议。