CORE D: DYNAMIC IMAGING FACILITY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 核心动态成像：为Cobre计划提供动态成像和分析服务。Core D提供设备和专业知识来进行钙离子/视频成像实验，并协助分析任何类型的成像数据。核心D有两个专门的钙成像室，每个都有一个完整的设置，以获取结合电生理和/或张力记录的钙电影。可以在上面进行实验 在室温或生理温度下，单个细胞或整个组织中。此外，其他装置还允许对完整或解剖组织的运动进行视频记录。为了以高效的方式分析数据，核心D具有中央文件服务器 以及用于长期存储和快速访问数据文件的RAID存储池。核心D的一个重要组成部分是能够对数据进行量身定做的分析，以回答PIS提出的具体问题。可使用内部分析程序(Volumeter G6a)进行所有分析，包括：运动稳定和 分析、时空地图分析、活动的协调/关联和三维重建。Core D为每个用户提供广泛的图像分析培训和优化实验的建议。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Core D- Dynamic imaging: provides dynamic imaging and analysis services tothe COBRE program. CORE D provides equipment and expertise to carry outCa2+/video imaging experiments and assists in the analysis of any type ofimaging data. CORE D has 2 dedicated Ca2+ imaging rooms, each with acomplete setup for acquiring Ca2+ movies combined with either electrophysiology and/or tension recordings. Experiments can be performed on single cells or in whole tissues at room or physiological temperatures. In addition, other setups allow video recordings of movements in intact or dissected tissues. In order to analyze data in an efficient manner, CORE D has a central file server and RAID storage pool for long term storage and quick access to data files. An important component of CORE D is the ability to tailor analysis of data to answer specific questions posed by the PIS. An in-house analysis program (Volumetry G6a) is available that is used for all analysis including: motion stabilization and analysis, spatio-temporal map analysis, coordination/correlation of activities and 3D reconstruction. CORE D provides each user with extensive image analysis training and recommendations for optimizing experiments.