MECHANISMS OF SEX DIFFERENTIATION IN MOUSE FETAL GERM CELLS

小鼠胎儿生殖细胞性别分化的机制

• 批准号: 8167753
• 负责人: YUKIKO YAMAZAKI
• 金额: $ 22.36万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167753
• 关键词: Adult; Beginning of Life; Cell Culture Techniques; Cell division; Cells; Chemicals; Computer Retrieval of Information on Scientific Projects Database; DNA; Data; Embryo; Embryonic Development; Event; Female; Fertilization; Funding; Germ Cells; Goals; Grant; Institution; Meiosis; Mus; Pathway interactions; Research; Research Personnel; Resources; Sex Differentiation; Somatic Cell; Source; Structure of primordial sex cell; Testing; United States National Institutes of Health; base; blastomere structure; egg; fetal; male; sex; sperm cell

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The primary goal of project is to understand the mechanisms of sex differentiation in primordial germ cells (PGCs), the embryonic cells that give rise to either sperm or egg cells in the adult. Current evidence suggests that PGCs in both female (XX) and male (XY) embryos begin life being bipotent, capable of differentiating along either the male or female pathway. The decision to differentiate to male or female germs cells is controlled by the gonadal somatic cells, the cells that surround the PGCs. But this has not been fully tested. After sex differentiation, male and female germ cells alter their DNA and begin cell division based on whether they have entered the male or female specific pathway. These sex-specific events are essential to produce functional sperm and eggs. Otherwise, normal fertilization and embryonic development do not occur correctly. However, we do not know the exact relationship between sex differentiation and the following sex-specific events in fetal germ cells. Our previous data demonstrate that in mice by 13.5 days after fertilization, PGCs control their own fate, and are no longer regulated by the surrounding somatic cells. In this project, we will first examine the relationship between sex differentiation and the following sex-specific events in germ cells cultured with external chemicals/factors. Next, we will test which conditions, if any, are required to direct PGCs into male or female pathway. Finally, we will determine if germ cells maintain intrinsic clock to enter meiosis by themselves in the absence of somatic support.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目的主要目标是了解原始生殖细胞（PGCs）的性别分化机制，原始生殖细胞是胚胎细胞，在成人中产生精子或卵细胞。 目前的证据表明，女性（XX）和男性（XY）胚胎中的PGCs开始生命是双能的，能够沿着男性或女性途径分化。 决定分化为男性或女性生殖细胞是由性腺体细胞，细胞周围的PGCs。 但这还没有得到充分的测试。 性别分化后，雄性和雌性生殖细胞会改变其DNA，并根据是否进入雄性或雌性特定途径而开始细胞分裂。这些性别特异性事件对于产生功能性精子和卵子至关重要。 否则，正常的受精和胚胎发育不会正确发生。 然而，我们不知道性别分化和以下性别特异性事件之间的确切关系在胎儿生殖细胞。 我们以前的数据表明，在受精后13.5天的小鼠中，PGCs控制自己的命运，不再受周围体细胞的调节。 在这个项目中，我们将首先研究与外部化学物质/因素培养的生殖细胞中性别分化和以下性别特异性事件之间的关系。 接下来，我们将测试哪些条件（如果有的话）需要将PGCs引导到男性或女性途径。 最后，我们将确定生殖细胞是否保持内在的时钟，在没有体细胞支持的情况下自行进入减数分裂。