IMAGING AND HISTOLOGY CORE

成像和组织学核心

• 批准号: 8167594
• 负责人: SCOTT Allen WETZEL
• 金额: $ 10.07万
• 依托单位: UNIVERSITY OF MONTANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167594
• 关键词: Biological; Computer Retrieval of Information on Scientific Projects Database; Computer software; Computers; Consult; Core Facility; Cultured Cells; Equipment; Equipment and supply inventories; Experimental Designs; Freezing; Funding; Grant; Histology; Image; Image Analysis; Imagery; Immunohistochemistry; Institution; Light; Light Microscope; Maintenance; Microscope; Microtome - medical device; Molecular; Montana; Optics; Paraffin Embedding; Proteins; Research; Research Personnel; Resources; Scientist; Services; Slide; Source; Staining method; Stains; Students; System; Techniques; Tissues; Training; United States National Institutes of Health; Universities; cellular imaging; charge coupled device camera; experience; finesse; fluorescence imaging; human tissue; immunocytochemistry; meetings; professor; programs; tissue culture; tissue fixing; tissue preparation; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ability to visualize and localize cellular proteins in tissues or cultured cells is an essential component of any biological program. The Molecular Histology and Fluorescence Imaging Core is dedicated to assisting researchers within the department, the University of Montana, and across the state with projects involving the identification, visualization, and localization of proteins in tissue culture as well as in tissue sections. The purpose of The MHFI core is to provide researchers with the resources to prepare tissue sections for histological and immunohistochemical staining. Toward this end the facility maintains the following equipment: + 2 microtomes for cutting paraffin embedded tissue- RMC MT 920 and Shandon Finesse 325 + 1 Shandon Citadel 2000 tissue processor + 1 Shandon Histocentre 2 embedding center + 1 Shandon Veristain 24-4 automated stainer for standard histology stains + 2 Cryotome cryostatic microtomes for frozen tissues  one for human tissue, one for non  human tissue + 1 vibratome for sectioning fixed tissues The core also maintains an available inventory of slides, coverslips, mounting media, waterbaths, and a variety of other histology tools. The core employs a staff scientist versed in histology and immunohistochemistry (IHC) techniques. The scientist consults with researchers on experimental design, maintains the core equipment, provides histology services and training to researchers and students, and other related services as needed. The MHFI provides researchers with analyzable images of cells/tissues. The core contains both the equipment and the technical assistance for acquiring and analyzing images taken from both the light and confocal microscope. The facility maintains the following equipment: + Olympus SZX16 Research Stereo Microscope, with a DP71- 12.5MP CCD Camera + 1 Nikon E800 research epifluorescent/transmitted light microscope with DIC optics + 1 Biorad Radiance 2000 confocal microscope on a Nikon TE300 inverted epifluor/ transmitted light microscope + 1 Nuance multi-spectral Image analysis system + NIH Imaging, Image J, CRi/Nuance Multispectral imaging, Image Pro Version 6.2 and Photoshop software + 3 Macintosh computers + 2 PC workstations The MHFI Core is under the direction of Dr. Diana Lurie (Professor, BMED). Dr. Lurie has 20 years of experience in both imaging and tissue preparation techniques including immunocytochemistry. She is the direct supervisor of Lou Herritt, the Staff Scientist for the MHFI core. Ms. Herritt is an experienced histologist with extensive training in imaging, including advanced coursework in confocal microscope techniques. Diane Brooks, B.S., provides additional technical support in the core. Dr. Lurie, Ms. Brooks and Ms. Herritt meet weekly to discuss the maintenance of the core facility as well as scientific projects currently ongoing in the core.

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 可视化和定位组织或培养细胞中的细胞蛋白的能力是任何生物程序的重要组成部分。 分子组织学和荧光成像核心致力于协助部门内的研究人员，蒙大拿大学和全州的项目涉及组织培养和组织切片中蛋白质的识别，可视化和定位。 MHFI核心的目的是为研究人员提供资源，以准备组织切片进行组织学和免疫组织化学染色。为此目的，该设施维持下列设备： + 2个切片机，用于切割石蜡包埋组织- RMC MT 920和Shandon Finesse 325 + 1台Shandon Citadel 2000组织处理机 + 1山东历史中心2植入中心 + 1台Shandon Veristain 24-4自动染色机，用于标准组织学染色 + 2个用于冷冻组织的冷冻切片机  一个用于人体组织，一个用于非  人体组织 + 1个振动刀，用于对固定组织进行切片 核心还保持了载玻片、盖玻片、封固剂、水浴和各种其他组织学工具的可用库存。该中心雇用了一名精通组织学和免疫组织化学（IHC）技术的科学家。 科学家与研究人员就实验设计进行协商，维护核心设备，为研究人员和学生提供组织学服务和培训，并根据需要提供其他相关服务。 MHFI为研究人员提供了可分析的细胞/组织图像。核心包括设备和技术援助，用于获取和分析从光学和共聚焦显微镜拍摄的图像。该机构维护以下设备： + Olympus SZX 16 Research立体显微镜，配备DP 71 - 12.5MP CCD摄像头 + 1台Nikon E800研究用落射/透射光显微镜， DIC光学 + 1台Biorad Radiance 2000共聚焦显微镜，尼康TE 300倒置 荧光/透射显微镜 + 1 Nuance多光谱图像分析系统 + NIH Imaging，Image J，CRi/Nuance多光谱成像，Image Pro版本 6.2和Photoshop软件 + 3台Macintosh电脑 + 2个PC工作站 MHFI核心是在Diana Lurie博士（教授， BMED）。 Lurie博士在成像和组织制备技术（包括免疫细胞化学）方面拥有20年的经验。 她是MHFI核心科学家Lou Herritt的直接主管。 Herritt女士是一位经验丰富的组织学家，在成像方面接受过广泛的培训，包括共聚焦显微镜技术的高级课程。 戴安布鲁克斯，学士，在核心中提供额外的技术支持。 Lurie博士、布鲁克斯女士和Herritt女士每周举行会议，讨论核心设施的维护以及目前正在核心进行的科学项目。