IMAGING AND HISTOLOGY CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在组织或培养细胞中对细胞蛋白质进行可视化和定位的能力是任何生物程序的基本组成部分。分子组织学和荧光成像核心致力于协助该系、蒙大拿大学和全州的研究人员开展涉及组织培养和组织切片中蛋白质的识别、可视化和定位的项目。MHFI核心的目的是为研究人员提供准备组织切片用于组织学和免疫组织化学染色的资源。为此，该设施维护以下设备： +2台切割石蜡包埋组织的显微镜--RMC MT 920和Shandon Finesse 325 +1个Shandon Citadel 2000组织处理器 +1个山东历史中心2个嵌入中心 +1台Shandon Veristain 24-4全自动组织学染色 +2台冷冻组织冷冻切割机，1台用于人体组织，1台用于非人体组织 +1个用于切开固定组织的振动器 核心还保留了一份可用的幻灯片、封面幻灯片、安装介质、水浴和各种其他组织学工具的库存。该中心聘请了一名精通组织学和免疫组织化学(IHC)技术的科学家。该科学家就实验设计向研究人员提供咨询，维护核心设备，为研究人员和学生提供组织学服务和培训，并根据需要提供其他相关服务。 MHFI为研究人员提供了细胞/组织的可分析图像。核心包括获取和分析从光学显微镜和共焦显微镜获取的图像的设备和技术援助。该设施维护以下设备： +奥林巴斯SZX16研究型立体显微镜，配备DP71-1250万像素CCD摄像头 +1尼康E800研究荧光/透射光显微镜，带 DIC光学 尼康TE300倒置+1 Biorad Radiance 2000共焦显微镜 荧光/透光显微镜 +1 Nuance多光谱图像分析系统 +NIH成像，图像J，CRI/Nuance多光谱成像，图像专业版 6.2和Photoshop软件 +3台麦金塔电脑 +2台PC工作站 MHFI核心由Diana Lurie博士(教授， BMed)。Lurie博士在成像和组织准备技术方面都有20年的经验，包括免疫细胞化学。她是Lou Herritt的直接主管，Lou Herritt是MHFI核心的员工科学家。赫里特女士是一位经验丰富的组织学家，在成像方面接受过广泛的培训，包括共焦显微镜技术方面的高级课程。戴安·布鲁克斯，B.S.，在核心部分提供额外的技术支持。Lurie博士、Brooks女士和Herritt女士每周会面，讨论核心设施的维护以及目前正在核心进行的科学项目。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ability to visualize and localize cellular proteins in tissues or cultured cells is an essential component of any biological program. The Molecular Histology and Fluorescence Imaging Core is dedicated to assisting researchers within the department, the University of Montana, and across the state with projects involving the identification, visualization, and localization of proteins in tissue culture as well as in tissue sections. The purpose of The MHFI core is to provide researchers with the resources to prepare tissue sections for histological and immunohistochemical staining. Toward this end the facility maintains the following equipment: + 2 microtomes for cutting paraffin embedded tissue- RMC MT 920 and Shandon Finesse 325 + 1 Shandon Citadel 2000 tissue processor + 1 Shandon Histocentre 2 embedding center + 1 Shandon Veristain 24-4 automated stainer for standard histology stains + 2 Cryotome cryostatic microtomes for frozen tissues  one for human tissue, one for non  human tissue + 1 vibratome for sectioning fixed tissues The core also maintains an available inventory of slides, coverslips, mounting media, waterbaths, and a variety of other histology tools. The core employs a staff scientist versed in histology and immunohistochemistry (IHC) techniques. The scientist consults with researchers on experimental design, maintains the core equipment, provides histology services and training to researchers and students, and other related services as needed. The MHFI provides researchers with analyzable images of cells/tissues. The core contains both the equipment and the technical assistance for acquiring and analyzing images taken from both the light and confocal microscope. The facility maintains the following equipment: + Olympus SZX16 Research Stereo Microscope, with a DP71- 12.5MP CCD Camera + 1 Nikon E800 research epifluorescent/transmitted light microscope with DIC optics + 1 Biorad Radiance 2000 confocal microscope on a Nikon TE300 inverted epifluor/ transmitted light microscope + 1 Nuance multi-spectral Image analysis system + NIH Imaging, Image J, CRi/Nuance Multispectral imaging, Image Pro Version 6.2 and Photoshop software + 3 Macintosh computers + 2 PC workstations The MHFI Core is under the direction of Dr. Diana Lurie (Professor, BMED). Dr. Lurie has 20 years of experience in both imaging and tissue preparation techniques including immunocytochemistry. She is the direct supervisor of Lou Herritt, the Staff Scientist for the MHFI core. Ms. Herritt is an experienced histologist with extensive training in imaging, including advanced coursework in confocal microscope techniques. Diane Brooks, B.S., provides additional technical support in the core. Dr. Lurie, Ms. Brooks and Ms. Herritt meet weekly to discuss the maintenance of the core facility as well as scientific projects currently ongoing in the core.