IMAGING AND HISTOLOGY CORE

成像和组织学核心

• 批准号: 8360464
• 负责人: SCOTT Allen WETZEL
• 金额: $ 9.78万
• 依托单位: UNIVERSITY OF MONTANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360464
• 关键词: Biological; Biological Sciences; British Columbia; Canada; Cells; Collection; Computer Workstations; Computer software; Computers; Consult; Core Facility; Cultured Cells; Environmental Health; Equipment; Equipment and supply inventories; Experimental Designs; Faculty; Fluorescence; Freezing; Funding; Grant; Histology; Image; Image Analysis; Imagery; Imaging Techniques; Immunohistochemistry; International; Lasers; Life; Light; Light Microscope; Maintenance; Microscope; Microscopy; Microtome - medical device; Molecular; Montana; National Center for Research Resources; Paraffin Embedding; Performance; Principal Investigator; Proteins; Research; Research Infrastructure; Research Personnel; Resources; Scanning; Scientist; Services; Slide; Source; Specimen; Staining method; Stains; Students; System; Techniques; Tissue Stains; Tissues; Training; United States National Institutes of Health; Universities; cellular imaging; cost; cryostat; digital imaging; experience; finesse; fluorescence imaging; instrument; meetings; member; professor; programs; research study; sample fixation; tissue culture

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The ability to visualize and localize cellular proteins in tissues or cultured cells is an essential component of any biological program. The Molecular Histology and Fluorescence Imaging Core is dedicated to assisting researchers within the department, the University of Montana, and across the state with projects involving the identification, visualization, and localization of proteins in tissue culture as well as in tissue sections. The Histology core provides the resources and expertise for preparing tissue for histological and immunohistochemical staining. We offer routine fixation, embedding, sectioning and staining of tissue specimens- either paraffin embedded or frozen tissue. The instruments present in Histology include: + RMT MT 920 & Shandon Finesse 325 rotary microtomes + Shandon Citadel 2000 tissue processor + Shandon Histocentre embedding station + Shandon Veristain 24-4 auto-stainer + Leica CM1950 cryostat The core also maintains an available inventory of slides, coverslips, mounting media, and waterbaths. The core employs a staff scientist versed in histology and immunohistochemistry (IHC) techniques. The scientist consults with researchers on experimental design, maintains the core equipment, provides histology services and training to researchers and students, and other related services as needed. Equipment use/ sign-up sheets are used in Histology. The Imaging component provides researchers with analyzable images of cells/ tissues taken from both the light and confocal microscopes. All of the microscopes in MHFI are interfaced with computers with proprietary software for collecting digital images. We also have copies of the software installed on workstation computers for post-collection analysis. Core Imaging instruments include: + Nikon E 800 epi-fluorescent/transmitted light microscope, with a Nuance multi-spectral imaging system + Nikon TE 300 inverted microscope with DIC and epi-fluorescence + Olympus FV 1000 IX inverted laser scanning confocal microscope with 405, 458,488,515, 559 & 635 nm laser lines + Olympus SZX16 fluorescence dissecting microscope, 1.25x obj + Software available includes Image J, Photoshop, Triple I Slidebook and Image Pro Plus V7.0 The facility is operated under the direction of Dr. Scott Wetzel (Assistant Professor, Division of Biological Sciences and Center for Environmental Health Sciences). Dr. Wetzel has 23 years of experience in advanced imaging techniques and for the last 11 years has served as a faculty member of the International Course on 3D Microscopy of Living Cells held at the University of British Columbia in Vancouver, Canada. He is the direct supervisor of Lou Herritt, the Staff Scientist for the MHFI core. Ms.Herritt is an experienced histologist with extensive training in imaging, including advanced coursework in confocal microscope techniques. Both Dr. Wetzel and Ms. Herritt meet with core users to assist in planning, performance and analysis of imaging experiments. Dr. Wetzel and Ms. Herritt meet weekly to discuss the maintenance of the core facility as well as scientific projects currently ongoing in the core. The MHFI Core is located in the Skaggs Building - Rooms 060C, 070 and 052A.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 在组织或培养细胞中可视化和定位细胞蛋白的能力是任何生物学程序的重要组成部分。分子组织学和荧光成像核心致力于协助该系，蒙大拿州大学以及整个州内的研究人员，涉及蛋白质在组织培养以及组织切片中的鉴定，可视化和定位的项目。 组织学核心提供了为组织学和免疫组织化学染色的组织准备的资源和专业知识。我们提供组织标本的常规固定，嵌入，切片和染色 - 嵌入或冷冻的组织。 组织学中存在的仪器包括： + RMT MT 920＆Shandon Finesse 325旋转微失位 + Shandon Citadel 2000组织处理器 + Shandon Histocentre嵌入站 + Shandon Veristain 24-4自动污渍 + Leica CM1950低温恒温器 该核心还保留了可用的幻灯片，盖玻片，安装媒体和水浴的清单。核心雇用专门在组织学和 免疫组织化学（IHC）技术。 科学家与研究人员进行实验设计协商，维护核心设备，为研究人员和学生以及其他相关服务提供组织学服务和培训。设备使用/注册表用于组织学。 成像组件为研究人员提供了从光和共聚焦显微镜中拍摄的细胞/组织的可分析图像。 MHFI中的所有显微镜都与用于收集数字图像的专有软件连接。我们还在工作站计算机上安装了该软件的副本以进行集合后分析。核心成像仪器包括： + Nikon E 800 Epi-fluorsent/transmented Light显微镜，带有细微的多光谱成像系统 + Nikon TE 300倒置显微镜，具有DIC和Epi-Fluorecence + Olympus FV 1000 IX倒激光扫描共聚焦显微镜，具有405、458,488,515、559和635 nm激光线 + Olympus SZX16荧光解剖显微镜，1.25x OBJ +可用的软件包括图像J，Photoshop，Triple I Slidebook和 图片Pro Plus V7.0 该设施是在Scott Wetzel博士（生物科学系助理教授和环境健康科学中心）的指导下运营的。 Wetzel博士在高级成像技术方面拥有23年的经验，在过去的11年中，一直是在加拿大温哥华不列颠哥伦比亚大学举行的3D活细胞中国际课程的教职员工。他是MHFI Core的参谋科学家Lou Herritt的直接主管。 Herritt女士是一位经验丰富的组织学家，对成像进行了广泛的培训，包括共聚焦显微镜技术的高级课程。 Wetzel博士和Herritt女士都与核心用户会面，以协助计划，性能和分析成像实验。 Wetzel博士和Herritt女士每周开会，讨论核心设施的维护以及目前正在核心的科学项目。 MHFI核心位于Skaggs大楼 - 房间060C，070和052A。