IMAGING AND HISTOLOGY CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在组织或培养细胞中对细胞蛋白质进行可视化和定位的能力是任何生物程序的基本组成部分。分子组织学和荧光成像核心致力于协助该系、蒙大拿大学和全州的研究人员开展涉及组织培养和组织切片中蛋白质的识别、可视化和定位的项目。 组织学核心提供了为组织学和免疫组织化学染色准备组织的资源和专业知识。我们提供组织标本的常规固定、包埋、切片和染色--无论是石蜡包埋组织还是冰冻组织。 组织学中存在的工具包括： +RMT 920和Shandon Finesse 325旋转缩微计 +Shandon Citadel 2000组织处理器 +山东历史中心嵌入站 +Shandon Veristain 24-4自动染染机 +徕卡CM1950低温恒温器 核心还保留了一份可用的幻灯片、封面、安装介质和水浴的库存。核心聘请了一名精通组织学和 免疫组织化学(IHC)技术。该科学家就实验设计向研究人员提供咨询，维护核心设备，为研究人员和学生提供组织学服务和培训，并根据需要提供其他相关服务。在组织学中使用设备使用/签名表。 成像组件为研究人员提供了从光学显微镜和共焦显微镜获取的细胞/组织的可分析图像。MHFI的所有显微镜都与装有收集数字图像的专有软件的计算机连接。我们还在工作站计算机上安装了该软件的副本，用于收集后分析。核心成像仪器包括： +尼康E 800表观荧光/透射式光学显微镜，配备Nuance多光谱成像系统 +NICON TE 300倒置显微镜，带DIC和表观荧光 +奥林巴斯FV 1000 IX倒置激光扫描共聚焦显微镜，具有405、458、488、515、559和635 nm激光谱线 +奥林巴斯SZX16荧光解剖显微镜，1.25x Obj +可用的软件包括Image J、Photoshop、Triple I Slidebook和 Image Pro Plus V7.0 该设施在Scott Wetzel博士(生物科学部和环境健康科学中心助理教授)的指导下运营。韦策尔博士在先进成像技术方面拥有23年的经验，在过去的11年里，他一直担任加拿大温哥华不列颠哥伦比亚大学活体细胞3D显微镜国际课程的教员。他是Lou Herritt的直接主管，Lou Herritt是MHFI核心的员工科学家。赫里特女士是一位经验丰富的组织学家，在成像方面接受过广泛的培训，包括共焦显微镜技术方面的高级课程。Wetzel博士和Herritt女士都与核心用户会面，协助计划、执行和分析成像实验。Wetzel博士和Herritt女士每周会面，讨论核心设施的维护以及目前正在核心进行的科学项目。 MHFI核心位于斯卡格斯大楼--060C、070和052A室。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The ability to visualize and localize cellular proteins in tissues or cultured cells is an essential component of any biological program. The Molecular Histology and Fluorescence Imaging Core is dedicated to assisting researchers within the department, the University of Montana, and across the state with projects involving the identification, visualization, and localization of proteins in tissue culture as well as in tissue sections. The Histology core provides the resources and expertise for preparing tissue for histological and immunohistochemical staining. We offer routine fixation, embedding, sectioning and staining of tissue specimens- either paraffin embedded or frozen tissue. The instruments present in Histology include: + RMT MT 920 & Shandon Finesse 325 rotary microtomes + Shandon Citadel 2000 tissue processor + Shandon Histocentre embedding station + Shandon Veristain 24-4 auto-stainer + Leica CM1950 cryostat The core also maintains an available inventory of slides, coverslips, mounting media, and waterbaths. The core employs a staff scientist versed in histology and immunohistochemistry (IHC) techniques. The scientist consults with researchers on experimental design, maintains the core equipment, provides histology services and training to researchers and students, and other related services as needed. Equipment use/ sign-up sheets are used in Histology. The Imaging component provides researchers with analyzable images of cells/ tissues taken from both the light and confocal microscopes. All of the microscopes in MHFI are interfaced with computers with proprietary software for collecting digital images. We also have copies of the software installed on workstation computers for post-collection analysis. Core Imaging instruments include: + Nikon E 800 epi-fluorescent/transmitted light microscope, with a Nuance multi-spectral imaging system + Nikon TE 300 inverted microscope with DIC and epi-fluorescence + Olympus FV 1000 IX inverted laser scanning confocal microscope with 405, 458,488,515, 559 & 635 nm laser lines + Olympus SZX16 fluorescence dissecting microscope, 1.25x obj + Software available includes Image J, Photoshop, Triple I Slidebook and Image Pro Plus V7.0 The facility is operated under the direction of Dr. Scott Wetzel (Assistant Professor, Division of Biological Sciences and Center for Environmental Health Sciences). Dr. Wetzel has 23 years of experience in advanced imaging techniques and for the last 11 years has served as a faculty member of the International Course on 3D Microscopy of Living Cells held at the University of British Columbia in Vancouver, Canada. He is the direct supervisor of Lou Herritt, the Staff Scientist for the MHFI core. Ms.Herritt is an experienced histologist with extensive training in imaging, including advanced coursework in confocal microscope techniques. Both Dr. Wetzel and Ms. Herritt meet with core users to assist in planning, performance and analysis of imaging experiments. Dr. Wetzel and Ms. Herritt meet weekly to discuss the maintenance of the core facility as well as scientific projects currently ongoing in the core. The MHFI Core is located in the Skaggs Building - Rooms 060C, 070 and 052A.