IMAGING AND HISTOLOGY CORE

成像和组织学核心

• 批准号: 8360464
• 负责人: SCOTT Allen WETZEL
• 金额: $ 9.78万
• 依托单位: UNIVERSITY OF MONTANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360464
• 关键词: Biological; Biological Sciences; British Columbia; Canada; Cells; Collection; Computer Workstations; Computer software; Computers; Consult; Core Facility; Cultured Cells; Environmental Health; Equipment; Equipment and supply inventories; Experimental Designs; Faculty; Fluorescence; Freezing; Funding; Grant; Histology; Image; Image Analysis; Imagery; Imaging Techniques; Immunohistochemistry; International; Lasers; Life; Light; Light Microscope; Maintenance; Microscope; Microscopy; Microtome - medical device; Molecular; Montana; National Center for Research Resources; Paraffin Embedding; Performance; Principal Investigator; Proteins; Research; Research Infrastructure; Research Personnel; Resources; Scanning; Scientist; Services; Slide; Source; Specimen; Staining method; Stains; Students; System; Techniques; Tissue Stains; Tissues; Training; United States National Institutes of Health; Universities; cellular imaging; cost; cryostat; digital imaging; experience; finesse; fluorescence imaging; instrument; meetings; member; professor; programs; research study; sample fixation; tissue culture

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The ability to visualize and localize cellular proteins in tissues or cultured cells is an essential component of any biological program. The Molecular Histology and Fluorescence Imaging Core is dedicated to assisting researchers within the department, the University of Montana, and across the state with projects involving the identification, visualization, and localization of proteins in tissue culture as well as in tissue sections. The Histology core provides the resources and expertise for preparing tissue for histological and immunohistochemical staining. We offer routine fixation, embedding, sectioning and staining of tissue specimens- either paraffin embedded or frozen tissue. The instruments present in Histology include: + RMT MT 920 & Shandon Finesse 325 rotary microtomes + Shandon Citadel 2000 tissue processor + Shandon Histocentre embedding station + Shandon Veristain 24-4 auto-stainer + Leica CM1950 cryostat The core also maintains an available inventory of slides, coverslips, mounting media, and waterbaths. The core employs a staff scientist versed in histology and immunohistochemistry (IHC) techniques. The scientist consults with researchers on experimental design, maintains the core equipment, provides histology services and training to researchers and students, and other related services as needed. Equipment use/ sign-up sheets are used in Histology. The Imaging component provides researchers with analyzable images of cells/ tissues taken from both the light and confocal microscopes. All of the microscopes in MHFI are interfaced with computers with proprietary software for collecting digital images. We also have copies of the software installed on workstation computers for post-collection analysis. Core Imaging instruments include: + Nikon E 800 epi-fluorescent/transmitted light microscope, with a Nuance multi-spectral imaging system + Nikon TE 300 inverted microscope with DIC and epi-fluorescence + Olympus FV 1000 IX inverted laser scanning confocal microscope with 405, 458,488,515, 559 & 635 nm laser lines + Olympus SZX16 fluorescence dissecting microscope, 1.25x obj + Software available includes Image J, Photoshop, Triple I Slidebook and Image Pro Plus V7.0 The facility is operated under the direction of Dr. Scott Wetzel (Assistant Professor, Division of Biological Sciences and Center for Environmental Health Sciences). Dr. Wetzel has 23 years of experience in advanced imaging techniques and for the last 11 years has served as a faculty member of the International Course on 3D Microscopy of Living Cells held at the University of British Columbia in Vancouver, Canada. He is the direct supervisor of Lou Herritt, the Staff Scientist for the MHFI core. Ms.Herritt is an experienced histologist with extensive training in imaging, including advanced coursework in confocal microscope techniques. Both Dr. Wetzel and Ms. Herritt meet with core users to assist in planning, performance and analysis of imaging experiments. Dr. Wetzel and Ms. Herritt meet weekly to discuss the maintenance of the core facility as well as scientific projects currently ongoing in the core. The MHFI Core is located in the Skaggs Building - Rooms 060C, 070 and 052A.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 可视化和定位组织或培养细胞中的细胞蛋白的能力是任何生物程序的重要组成部分。分子组织学和荧光成像核心致力于协助该系、蒙大拿大学和全州的研究人员开展涉及组织培养物和组织切片中蛋白质的识别、可视化和定位的项目。 组织学核心提供了用于组织学和免疫组织化学染色的组织准备的资源和专业知识。我们提供组织标本（石蜡包埋或冷冻组织）的常规固定、包埋、切片和染色。 组织学中的仪器包括： + RMT MT 920 和 Shandon Finesse 325 旋转切片机 + Shandon Citadel 2000 组织处理机 + Shandon Histocentre 包埋站 + Shandon Veristin 24-4 自动染色机 + Leica CM1950 低温恒温器 该核心还维护载玻片、盖玻片、封固剂和水浴的可用库存。核心聘用了一位精通组织学和 免疫组织化学 (IHC) 技术。 科学家就实验设计向研究人员进行咨询，维护核心设备，为研究人员和学生提供组织学服务和培训，以及根据需要提供其他相关服务。设备使用/登记表用于组织学。 成像组件为研究人员提供从光学显微镜和共焦显微镜拍摄的可分析的细胞/组织图像。 MHFI 中的所有显微镜都与带有专有软件的计算机连接，用于收集数字图像。我们还在工作站计算机上安装了该软件的副本，用于收集后分析。核心成像仪器包括： + Nikon E 800 落射荧光/透射光显微镜，配有 Nuance 多光谱成像系统 + Nikon TE 300 倒置显微镜，带 DIC 和落射荧光 + Olympus FV 1000 IX 倒置激光扫描共焦显微镜，具有 405、458,488,515、559 和 635 nm 激光线 + Olympus SZX16 荧光解剖显微镜，1.25 倍物镜 + 可用软件包括 Image J、Photoshop、Triple I Slidebook 和 图像专业增强版V7.0 该设施在 Scott Wetzel 博士（生物科学部和环境健康科学中心助理教授）的指导下运营。 Wetzel 博士在先进成像技术方面拥有 23 年经验，过去 11 年一直担任加拿大温哥华不列颠哥伦比亚大学活细胞 3D 显微镜国际课程的教员。他是 MHFI 核心研究员 Lou Herritt 的直接主管。 Herritt 女士是一位经验丰富的组织学家，接受过广泛的成像培训，包括共焦显微镜技术的高级课程。 Wetzel 博士和 Herritt 女士都会与核心用户会面，协助成像实验的规划、执行和分析。 韦策尔博士和赫里特女士每周会面讨论核心设施的维护以及目前在核心正在进行的科学项目。 MHFI 核心位于 Skaggs 大楼 - 060C、070 和 052A 室。