FUNCTIONAL GENOMICS OF ENDOCRINE DISRUPTION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，但不一定是研究者所在的机构。 研究蝾螈内分泌干扰的生态学与功能基因组学的互补，将允许开发一种可用于人类环境健康政策的两栖动物艾德生物测定系统。 使用微阵列分析和随后的原位杂交可以识别与阿特拉津暴露相关的差异基因表达。 当这种基因组学方法辅之以对各种条件的生态操作时，它允许确定一组可能的假设，即什么条件导致ED介导的基因上调和下调。 阿特拉津和雌激素对性成熟有明显的影响，但也与调节两栖动物变态的激素相互作用。 本提案中所述的工作旨在检验一种假设，即环境中常见的两种内分泌干扰物（阿特拉津和雌激素）的作用将与蝾螈幼虫所经历的正常生态线索相互作用。 ED与生态学相互作用的结果是，亚致死浓度的ED对蝾螈幼体的死亡率、生长、变态和性发育的影响将大于预期。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Examination of the ecology complemented with functional genomics of endocrine disruption in a salamander will allow development of an amphibian ED bioassy system that can be used in human environmental health policy. Use of microarray analysis and subsequent in situ hybridization can identify differential gene expression associated with atrazine exposure. When this genomics approach is complemented by ecological manipulations of a broad array of conditions, it allows for identification of a likely set of hypotheses for what conditions are resulting in ED-mediated up- and down-regulation of genes. Atrazine and estrogen have obvious effects on sexual maturation, but also interact with the hormones that regulate amphibian metamorphosis. The work described in this proposal is designed to test the hypothesis that the action of two endocrine disruptors (atrazine and estrogen) found commonly in the environment will interact with the normal ecological cues experienced by larval salamanders. The result of such interactions of EDs and ecology will be larger-than-expected effects of sub-lethal concentration of EDs on mortality, growth and metamorphic and sexual development of the larval salamanders.