METABOLIC CORE

代谢核心

• 批准号: 8016442
• 负责人: JACOB E FRIEDMAN
• 金额: $ 22.38万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8016442
• 关键词: 1-Phosphatidylinositol 3-Kinase; Animals; Applications Grants; Biological Assay; Budgets; Cancer Center; Cell Nucleus; Cells; Cellular Assay; Charge; Clinical; Collaborations; Colorado; Computer software; Consult; Consultations; Core Facility; Cost Savings; Diabetes Mellitus; Disease; Electrophoresis; Equipment; Exposure to; Fatty acid glycerol esters; Floor; Funding; Gene Expression; Genus Hippocampus; Grant; Histocompatibility Testing; Hormones; Human; Inflammation; Inflammatory Response; Insulin; Insulin Resistance; Investigation; Laboratories; Link; Liver; Malignant Neoplasms; Measures; Metabolic; Metabolism; Mitochondria; Molecular; Mus; Muscle; Nutritional Study; Obesity; Periderm; Phosphorylation; Rattus; Reading; Reagent; Reporting; Research; Research Personnel; Rodent; Scientist; Services; Sheep; Signaling Protein; Techniques; Time; Translations; U-Series Cooperative Agreements; United States National Institutes of Health; Universities; Weight Gain; Western Blotting; adipokines; animal data; base; clinical practice; cost; cytokine; design; energy balance; extracellular; fetal; instrument; insulin sensitivity; insulin signaling; interest; lipid metabolism; member; molecular array; nutrition; receptor; research study; time use

The overall theme of the Metabolic Core lab is to support investigation of insulin resistance, obesity, and inflammation from a cellular/molecular perspective. The Metabolic Core provides consultation and analysis of insulin signaling, hormone assays, qPCR, insulin clamping (mice, rats), and cell based assays of cytokines and adipokines tailored to the investigators needs. Over the last grant period the Metabolic Core has developed expertise in multiplex based cytokine analysis. Most recently the Metabolic Core received NIH funding to purchase a Seahorse XF24-3 extracellular flux analyzer to allow us to begin to interrogate mitochondrial function.

代谢核心实验室的总体主题是支持胰岛素抵抗，肥胖，