Flow Cytometry

CoRec 拟议的杜克大学CFAR流式细胞术核心设施将a)为杜克大学CFAR成员进行的基础研究或翻译研究提供最先进的、多颜色的、BSL-3荧光激活的细胞分类支持，包括对HIV-1感染细胞进行分类；b)为杜克大学CFAR成员进行的基础、翻译和临床研究提供最先进的、多颜色的BSL-2*流式细胞仪分析支持，以及c)通过教育核心用户更有效地利用杜克CFAR流式细胞术核心设施提供的最先进工具，以及为CFAR流式细胞术核心设施的管理人员和运营者提供方案改进，加强杜克CFAR调查人员使用的科学方法。BSL-3分选组件实验室将容纳杜克大学医学中心唯一能够分选感染HIV-1或同等病原体的活的、未固定的人或动物来源细胞的流式细胞仪，并将被证明是拟议的杜克CFAR流式细胞仪核心设施的无价资产。杜克大学的CFAR流式细胞术核心将是杜克大学唯一能够为杜克大学的艾滋病毒和艾滋病毒相关翻译研究提供多色和流动信息支持的流式细胞仪设施。拟议的杜克大学CFAR流式细胞仪核心设施将由两个组件实验室组成，两个实验室分别位于SORF和全球卫生大楼内的生物遏制实验室。组件实验室将在物理、管理和科学上紧密联系在一起，以形成一个高度互动和统一的杜克CFAR流式细胞仪核心设施。BSL-2*分析成分实验室将为CFAR调查人员提供广泛的多色流式细胞仪应用，并与BSL-3分类成分实验室一起，构成校园内唯一例行处理、分类和分析HIV-1感染样本的流式细胞仪研究设施。

CoreC The proposed Duke CFAR Flow Cytometry Core Facility will a) provide state-of-the-art, multi-color, BSL-3 fluorescence activated cell sorting support for basic research or translational investigations conducted by Duke CFAR members, including sorting HIV-1 infected cells, b) provide state-of-the-art, multi-color, BSL-2* flow cytometric analytical support for basic, translational, and clinical research investigations conducted by Duke CFAR members, and c) enhance the scientific approach used by Duke CFAR Investigators by educating the Core User base in more effective ways of utilizing the state-of-the-art tools offered by the Duke CFAR Flow Cytometry Core Facility, as well as to provide programmatic advancement for Managers and Operators of the CFAR Flow Cytometry Core Facility. The BSL-3 Sorting Component Laboratory will house the only flow cytometer at Duke University Medical Center capable of sorting live, unfixed human or animal derived cells, infected with HIV-1 or equivalent pathogens, and will prove an invaluable asset to the overall scope of the proposed Duke CFAR Flow Cytometry Core Facility. The Duke CFAR Flow Cytometry Core will be the only flow cytometry facility at Duke capable of providing polychromatic and flow informatic support for HIV and HIV-related translational research at Duke. The proposed Duke CFAR Flow Cytometry Core Facility will be comprised of two Component Laboratories, both housed in biocontainment laboratories within the SORF & Global Health Buildings, respectively. The Component Laboratories will be closely linked physically, administratively, and scientifically, in order to form a highly interactive and unified Duke CFAR Flow Cytometry Core Facility. The BSL-2* Analytical Component Laboratory will provide a broad range of polychromatic flow cytometry applications to CFAR investigators and, together with the BSL-3 Sorting Component Laboratory, comprise the only flow cytometry research facility on campus that routinely processes, sorts, and analyzes HIV-1 infected specimens.