Flow Cytometry

Corec 拟议的杜克CFAR流式细胞术核心设施将a）为杜克CFAR成员进行的基础研究或转化研究提供最先进的多色BSL-3荧光激活细胞分选支持，包括分选HIV-1感染细胞，B）为基础、转化、和杜克CFAR成员进行的临床研究调查，以及c）通过教育核心用户群更有效地利用杜克CFAR流式细胞术核心机构提供的最先进工具，增强杜克CFAR研究者使用的科学方法，以及为CFAR流式细胞术核心设施的管理者和操作者提供程序化的进步。BSL-3分选组件实验室将容纳杜克大学医学中心唯一的流式细胞仪，该流式细胞仪能够分选感染HIV-1或同等病原体的活的、未固定的人或动物源细胞，并将证明是拟议的杜克CFAR流式细胞仪核心设施整体范围的宝贵资产。杜克CFAR流式细胞术核心将是杜克唯一能够为杜克的HIV和HIV相关转化研究提供多色和流式信息支持的流式细胞术设施。拟议的杜克CFAR流式细胞术核心设施将由两个组件实验室组成，分别位于SORF和全球卫生大楼内的生物防护实验室内。组件实验室将在物理上、行政上和科学上紧密联系，以形成高度互动和统一的杜克CFAR流式细胞术核心设施。BSL-2* 分析成分实验室将为CFAR研究人员提供广泛的多色流式细胞术应用，并与BSL-3分选成分实验室一起，组成校园内唯一的流式细胞术研究设施，定期处理，分选和分析HIV-1感染标本。

CoreC The proposed Duke CFAR Flow Cytometry Core Facility will a) provide state-of-the-art, multi-color, BSL-3 fluorescence activated cell sorting support for basic research or translational investigations conducted by Duke CFAR members, including sorting HIV-1 infected cells, b) provide state-of-the-art, multi-color, BSL-2* flow cytometric analytical support for basic, translational, and clinical research investigations conducted by Duke CFAR members, and c) enhance the scientific approach used by Duke CFAR Investigators by educating the Core User base in more effective ways of utilizing the state-of-the-art tools offered by the Duke CFAR Flow Cytometry Core Facility, as well as to provide programmatic advancement for Managers and Operators of the CFAR Flow Cytometry Core Facility. The BSL-3 Sorting Component Laboratory will house the only flow cytometer at Duke University Medical Center capable of sorting live, unfixed human or animal derived cells, infected with HIV-1 or equivalent pathogens, and will prove an invaluable asset to the overall scope of the proposed Duke CFAR Flow Cytometry Core Facility. The Duke CFAR Flow Cytometry Core will be the only flow cytometry facility at Duke capable of providing polychromatic and flow informatic support for HIV and HIV-related translational research at Duke. The proposed Duke CFAR Flow Cytometry Core Facility will be comprised of two Component Laboratories, both housed in biocontainment laboratories within the SORF & Global Health Buildings, respectively. The Component Laboratories will be closely linked physically, administratively, and scientifically, in order to form a highly interactive and unified Duke CFAR Flow Cytometry Core Facility. The BSL-2* Analytical Component Laboratory will provide a broad range of polychromatic flow cytometry applications to CFAR investigators and, together with the BSL-3 Sorting Component Laboratory, comprise the only flow cytometry research facility on campus that routinely processes, sorts, and analyzes HIV-1 infected specimens.