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FUNCTIONAL GENOMICS CORE

功能基因组核心

基本信息

批准号：
7777675
负责人：
BRIAN Keith DIECKGRAEFE
金额：
$ 11.49万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-12-01 至 2014-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7777675
关键词：
Address Apoptosis Area Binding Bioinformatics Biological Process Budgets Cells Chemotaxis Clinical Collection Commit Communities Complex Core Facility DNA Microarray Chip DNA-Binding Proteins Databases Development Digestive System Disorders Disease Equipment Event Expressed Sequence Tags Funding Fungal Genome Gene Expression Genes Genetic Transcription Genome Hepatic Human Human Genome Project Human Resources Inflammation Institution Investigation Investments Laboratories Libraries Ligands Logic Malignant Neoplasms Matrix Metalloproteinases Measures Methodology Methods MicroRNAs Molecular Profiling Monitor Morphology Mus National Institute of General Medical Sciences Oligonucleotides Pathology Physiological Processes Polysaccharides Process Protein Array Protein-Carbohydrate Interaction Rattus Research Research Personnel Resources Role Sampling Services Streptavidin Structure Technology Time Tissue Banking Tissue Banks Tissue Procurements Tissues Training Universities Virus Diseases Washington Work angiogenesis arm base cDNA Arrays chromatin immunoprecipitation cost cost effective density design experience functional genomics functional group gastrointestinal genome sequencing instrumentation lipid metabolism programs promoter

项目摘要

The Human Genome Project and the development of the expressed sequence tag (EST) clone collection and database have revolutionized gene expression analysis. Instead of measuring one or a few genes, parallel DNA microarrays are capable of simultaneously measuring expression of thousands of genes, providing a glimpse into the logic and functional grouping of gene programs encoded by our genome. The term functional genomics encompasses varied approaches that provide a more global investigation into the expression and role of groups of genes in a physiologic process than has been previously available. As such, this approach represents a fundamental shift from the traditional 'one gene at a time' approach to the study of basic biological processes. cDNA microarrays and oligonucleotide-based microarray methodologies have the potential to provide a new level of information about cell or tissue function not previously possible. However, these technologies require an investment in expensive equipment and highly trained, experienced technicians. Most laboratories cannot afford to commit the substantial technical resources and personnel to the development and ongoing refinement of these particularly complex experimental methods. It is more cost effective to concentrate this instrumentation and highly skilled technical support in a centrally managed facility, where they will be efficiently utilized and cost effective. The Functional Genomics Core Facility was designed to provide this critical core expertise to the digestive disease research community. The unique feature of the Functional Genomics Core Facility is the expertise it provides to participating investigators on the application of high density comprehensive arrays to study gastrointestinal and hepatic qene expression. In maintaining an independent microarray core, DDRCC research investigators are provided with access to the Agilent platforms at extremely low costs ($275/2 sample comparisons using the 44K gene arrays) and with much more rapid turn around time (<5 days) than other microarray facilities in the institution. The close proximity of the Functional Genomics Core to the DDRCC Morphology Core and the DDRCC Tissue Procurement Facility greatly facilitates the processing and analysis of clinical samples related to digestive disease disorders. The DDRCC Functional Genomics Core works closely with other microarray facilities at this institution that offer alternative commercial platforms, such as the Affymetrix platforms supported by the CTSA sponsored Translational Pathology and Tissue Banking Core, and the Illumina platforms supported by the Genome Sequencing Center of Washington University. Important for tight budgets, the cost of our services is less than half the cost of a single sample Affy array, and is about 20% less than the Illumina platform. Just as important, the Functional Genomics Core provides integrative and bioinformatic services.

人类基因组计划和表达序列标签 (EST) 克隆集合的开发和数据库彻底改变了基因表达分析。不是测量一个或几个基因，而是平行测量 DNA 微阵列能够同时测量数千个基因的表达，提供一睹我们基因组编码的基因程序的逻辑和功能分组。术语功能性基因组学涵盖了多种方法，可以对表达和表达进行更全面的研究基因组在生理过程中的作用比以前可用的更深入。因此，这种方法代表了从传统的“一次一个基因”方法到基础生物学研究的根本转变流程。 cDNA 微阵列和基于寡核苷酸的微阵列方法有潜力提供有关细胞或组织功能的新水平的信息，这在以前是不可能的。然而，这些技术需要投资昂贵的设备和训练有素、经验丰富的技术人员。最多实验室无法投入大量的技术资源和人员来开发和不断完善这些特别复杂的实验方法。集中精力更划算这些仪器和高技能的技术支持在一个集中管理的设施中，他们将在那里有效利用且具有成本效益。功能基因组学核心设施旨在提供这一服务消化疾病研究界的关键核心专业知识。功能基因组学核心设施的独特之处在于它为参与项目提供的专业知识研究人员应用高密度综合阵列研究胃肠道和肝脏 qene 表达式。在维持独立的微阵列核心方面，DDRCC 研究人员提供了可以以极低的成本访问安捷伦平台（使用 44K 基因进行 275 美元/2 个样品比较阵列），并且比该机构中的其他微阵列设施具有更快的周转时间（<5 天）。功能基因组学核心与 DDRCC 形态学核心和 DDRCC 组织非常接近采购设施极大地方便了消化相关临床样本的处理和分析疾病失调。 DDRCC 功能基因组核心与其他微阵列设施密切合作提供替代商业平台的机构，例如 CTSA 支持的 Affymetrix 平台赞助的转化病理学和组织库核心，以及由华盛顿大学基因组测序中心。对于预算紧张来说很重要，我们的服务成本是单个样品 Affy 阵列的成本不到一半，比 Illumina 平台低约 20%。正如重要的是，功能基因组学核心提供综合和生物信息服务。