FUNCTIONAL GENOMICS CORE

人类基因组计划与表达序列标签（EST）克隆库的发展 和数据库已经彻底改变了基因表达分析。而不是测量一个或几个基因，平行 DNA微阵列能够同时测量数千个基因的表达， 一窥我们基因组编码的基因程序的逻辑和功能分组。术语功能 基因组学包括各种方法，这些方法提供了对表达的更全面的研究， 基因组在生理过程中的作用比以前可用。因此，这种方法 代表了从传统的“一次一个基因”的方法到基础生物学研究的根本转变。 流程. cDNA微阵列和基于寡核苷酸的微阵列方法具有潜力， 提供关于细胞或组织功能的新水平的信息，这在以前是不可能的。但这些 技术需要投资于昂贵的设备和训练有素、经验丰富的技术人员。最 实验室无法承担大量的技术资源和人员的开发， 不断完善这些特别复杂的实验方法。集中精力更划算 这种仪器和高度熟练的技术支持，在一个集中管理的设施，他们将 高效利用和成本效益。功能基因组学核心设施旨在提供这一点， 消化系统疾病研究界的关键核心专业知识。 功能基因组学核心设施的独特之处在于它为参与者提供的专业知识。 应用高密度综合阵列研究胃肠道和肝脏的研究者 qene表达式。在维持独立的微阵列核心方面，DDRCC研究调查员被提供 以极低的成本访问Agilent平台（使用44K基因进行2个样品比较， 阵列）并且具有比该机构中的其他微阵列设施快得多的周转时间（<5天）。 功能基因组学核心与DDRCC形态学核心和DDRCC组织的紧密接近 采购设施极大地促进了消化相关临床样本的处理和分析 疾病紊乱。DDRCC功能基因组学核心与其他微阵列设施密切合作， 提供替代商业平台的机构，如CTSA支持的Affymbox平台 赞助的转化病理学和组织库核心，以及由 华盛顿大学基因组测序中心。对于预算紧张的情况，我们的服务成本非常重要， 不到单个样品Affy阵列成本的一半，并且比Illumina平台低约20%。正如 重要的是，功能基因组学核心提供综合和生物信息学服务。

The Human Genome Project and the development of the expressed sequence tag (EST) clone collection and database have revolutionized gene expression analysis. Instead of measuring one or a few genes, parallel DNA microarrays are capable of simultaneously measuring expression of thousands of genes, providing a glimpse into the logic and functional grouping of gene programs encoded by our genome. The term functional genomics encompasses varied approaches that provide a more global investigation into the expression and role of groups of genes in a physiologic process than has been previously available. As such, this approach represents a fundamental shift from the traditional 'one gene at a time' approach to the study of basic biological processes. cDNA microarrays and oligonucleotide-based microarray methodologies have the potential to provide a new level of information about cell or tissue function not previously possible. However, these technologies require an investment in expensive equipment and highly trained, experienced technicians. Most laboratories cannot afford to commit the substantial technical resources and personnel to the development and ongoing refinement of these particularly complex experimental methods. It is more cost effective to concentrate this instrumentation and highly skilled technical support in a centrally managed facility, where they will be efficiently utilized and cost effective. The Functional Genomics Core Facility was designed to provide this critical core expertise to the digestive disease research community. The unique feature of the Functional Genomics Core Facility is the expertise it provides to participating investigators on the application of high density comprehensive arrays to study gastrointestinal and hepatic qene expression. In maintaining an independent microarray core, DDRCC research investigators are provided with access to the Agilent platforms at extremely low costs ($275/2 sample comparisons using the 44K gene arrays) and with much more rapid turn around time (<5 days) than other microarray facilities in the institution. The close proximity of the Functional Genomics Core to the DDRCC Morphology Core and the DDRCC Tissue Procurement Facility greatly facilitates the processing and analysis of clinical samples related to digestive disease disorders. The DDRCC Functional Genomics Core works closely with other microarray facilities at this institution that offer alternative commercial platforms, such as the Affymetrix platforms supported by the CTSA sponsored Translational Pathology and Tissue Banking Core, and the Illumina platforms supported by the Genome Sequencing Center of Washington University. Important for tight budgets, the cost of our services is less than half the cost of a single sample Affy array, and is about 20% less than the Illumina platform. Just as important, the Functional Genomics Core provides integrative and bioinformatic services.