PROTEOMIC CHARACTERIZATION OF CEREBROSPINAL FLUID BY HIGH RESOLUTION LC-MS/MS

通过高分辨率 LC-MS/MS 进行脑脊液蛋白质组学表征

• 批准号: 8170699
• 负责人: H Shaw Warren
• 金额: $ 3.21万
• 依托单位: BATTELLE PACIFIC NORTHWEST LABORATORIES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170699
• 关键词: Brain; Brain Diseases; Cerebrospinal Fluid; Clinical Medicine; Computer Retrieval of Information on Scientific Projects Database; Databases; Disease; Funding; Future; Gel; Goals; Grant; Human; Institution; Knowledge; Measures; Monitor; National Center for Research Resources; Proteins; Proteome; Proteomics; Research; Research Personnel; Resolution; Resources; Sampling; Source; Spinal Cord; Techniques; Time; United States National Institutes of Health; base; protein distribution

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This NCRR collaborative project has focused on the proteome characterization of human cerebrospinal fluid (CSF). CSF is routinely sampled in clinical medicine to help determine and monitor the presence of illness in the brain or spinal cord. Clinicians usually measure the total protein concentration for this purpose since very little is known about the protein composition, or proteome, of CSF in normal or disease states. Much of the current knowledge about CSF is based upon 2D gel analysis techniques which are severely limited in both sensitivity and throughput. The overall goals of the research proposed in this study are twofold: (1) determine, as possible, the content and distribution of proteins in "normal" human CSF for the creation of an accurate mass and time (AMT) tag database for future quantitative, high sensitivity LC-MS studies; and (2) evaluate how the content and abundance of proteins in CSF are altered in specific diseases of the brain and spinal cord.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这个NCRR合作项目的重点是人类脑脊液(CSF)的蛋白质组学特征。临床医学中经常对脑脊液进行采样，以帮助确定和监测大脑或脊髓中是否存在疾病。临床医生通常为此目的测量总蛋白浓度，因为人们对正常或疾病状态下脑脊液的蛋白质组成或蛋白质组知之甚少。目前关于脑脊液的大部分知识都是基于2D凝胶分析技术，这些技术在灵敏度和吞吐量方面都受到严重限制。这项研究提出的总体目标有两个：(1)尽可能地确定“正常”人脑脊液中蛋白质的含量和分布，以便为未来的定量、高灵敏度的LC-MS研究创建准确的质量和时间(AMT)标签数据库；(2)评估脑脊液中蛋白质的含量和丰度在特定的脑和脊髓疾病中是如何改变的。